| Project/Area Number |
25550096
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Design and evaluation of sustainable and environmental conscious system
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| Research Institution | Prefectural University of Kumamoto |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TANAKA Kenji 近畿大学, 産業理工学部, 教授 (20236582)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 生分解性プラスチック / 二酸化炭素 / ポリヒドロキシアルカン酸 / 環境技術 / 環境材料 |
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| Outline of Final Research Achievements |
The aim of this study is the biosynthesis of biodegradable copolyesters by Ralstonia eutropha from carbon dioxide. PHA synthase (PhaC1) and 3-hydroxyacyl-ACP:CoA transferase (thioesterase) (PhaG) genes from Pseudomonas sp. 61-3, (R)-3-hydroxyacyl-CoA ligase gene (PA3924) from Pseudomonas aeruginosa, and beta-ketotiolase (PhbA) and acetoacetyl-CoA reductase (PhbB) genes from R. eutropha were introduced into <i>Escherichia coli and PHA-negative R. eutropha strains. The copolymer containing 94.6 mol% 3-hydroxybutyrate (3HB) and 5.4 mol% medium-chain-length-3-hydroxyalkanoates (3HA) consisting of C8, C10, C12 and C14 was synthesized by the recombinant strains of E. coli from glucose as the sole carbon source.
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