Improvement of nutritional properties in soybean by branched amino acid signaling
Project/Area Number |
25560038
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Eating habits
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Research Institution | University of Miyazaki |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
INOUE Mari (IWAYA Mari) 九州大学, 農学研究科点, 教授 (60001394)
井上 眞理 九州大学, (連合)農学研究科(研究院), 教授 (60091394)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | アミノ酸 / 栄養シグナル / オートファジー / ダイズ / 転写因子 / ATG8 / BCAT / bZIP型転写因子 / 分岐鎖アミノ酸 / 糖シグナル / ゲノム |
Outline of Final Research Achievements |
Sucrose starvation treatment signi,cantly enhanced the expressions of GmbZIP53A, but not GmbZIP53B asparagine synthase (GmASN), proline dehydrogenase (GmProDH), and branched chain amino acid transaminase (GmBCAT). GmbZIP53-related immunoreactive signals were upregulated under severe starvation with sucrose starvation and protease inhibitors, while sucrose and sucrose starvation had no or marginal eects on the signal. Pro,les of induction of GmASN, GmProDH and GmBCAT3 under various nutrient conditions were consistent with the profles of GmbZIP53 protein levels but not with those of GmbZIP mRNA levels. These results indicate that GmbZIP53 proteins levels are regulated by posttranslational mechanism in response to severe starvation stress and that the increased protein of GmbZIP53 under severe starvation accelerates transcriptional induction of GmASN, GmProDH, and GmBCAT.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Molecular Mechanisms of flowering suppression of a drought tolerant legume, cowpea, in response to water deficient stress2014
Author(s)
Takashi YUASA, Masakazu IMAMURA, Ayami KANEKO, Eri NOGUCHI, Takahiro SAKAMOTO, Ai UCHIBAE, Yuya HASHIGUCHI, Eriko KURAUCHI, Megumi ARIMURA, Yushi ISHIBASHI, Mari IWAYA-INOUE
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Journal Title
Proceedings of the 50th Conference on the Biology of Halophilic Microorganisms
Volume: 50
Pages: 11-14
Related Report
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[Journal Article] Induction of a bZIP type transcription factor and amino acid catabolism-related genes in soybean seedling in response to starvation stress.2013
Author(s)
Yuasa, T., Nagasawa, K., Osanai, A., Kaneko, A., Tajima, D., Nang, MPSH., Ishibashi, Y. and Iwaya-Inoue, M.
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Journal Title
J. Bot.
Volume: 2013
Pages: 1-8
DOI
Related Report
Peer Reviewed
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