Circulating-miRNA in peripheral blood in metabolic state of between distant organs

• Project/Area Number: 25560055
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Nagasaki University
• Principal Investigator: MORI Ryoichi 長崎大学, 医歯薬学総合研究科(医学系), 講師 (30509310)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: miRNA / 代謝 / 高脂肪 / 寿命 / 老化 / 生細胞ライブイメージング

Outline of Final Research Achievements

Circulating miRNAs (c-miRNAs) is involved in metabolic state of between distant organs, however its functions have not been elucidated. In this study, to clarify functions of intercellular and extracellular inflammation-related miRNA in vivo, I have recently generated miRNA knockout (KO) mice and while they are grossly normal through embryogenesis, they are very vulnerable to live at early postnatal stages. Those individuals that make it through the neonatal period thrive and are fertile as adults and display no gross abnormalities. these results indicated that miRNA is contributed to homeostatic mechanism and other biological phenomenon such as inflammation and tissue repair.

Research Products

• [Journal Article] The life-extending effect of dietary restriction requires Foxo3 in mice (2015)
  • Author(s): Shimokawa I, Komatsu T, Hayashi N, Kim SE, Kawata T, Park S, Hayashi H, Yamaza H, Chiba T, Mori R
  • Journal Title: Aging Cell Volume: - Issue: 4 Pages: 707-707
  • DOI: 10.1111/acel.12340
  • Peer Reviewed / Open Access
• [Journal Article] Overexpression of the adiponectin gene mimics the metabolic and stress resistance effects of calorie restriction, but not the anti-tumor effect (2015)
  • Author(s): Kamohara R, Yamaza H, Tsuchiya T, Komatsu T, Park S, Hayashi H, Chiba T, Mori R, Otabe S, Yamada K, Nagayasu T, Shimokawa I
  • Journal Title: Experimental Gerontology Volume: 64 Pages: 46-54
  • DOI: 10.1016/j.exger.2015.02.011
  • NAID: 120006987178
  • Peer Reviewed / Open Access
• [Journal Article] NPY antagonism reduces adiposity and attenuates age-related imbalance of adipose tissue metabolism (2014)
  • Author(s): Park S, Fujishita C, Komatsu T, Kim SE, Chiba T, Mori R, Shimokawa I.
  • Journal Title: FASEB J Volume: 28 Issue: 12 Pages: 5337-48
  • DOI: 10.1096/fj.14-258384
  • Peer Reviewed
• [Journal Article] Reduced FOXO1 accelerates skin wound healing and attenuates scarring. (2014)
  • Author(s): Mori R, Tanaka K, de Kerckhove M, Okamoto M, Kashiyama K, Tanaka K, Kim SE, Kawata T, Komatsu T, Park S, Ikematsu K, Hirano A, Martin P, Shimokawa I
  • Journal Title: American Journal of Pathology Volume: 184 Issue: 9 Pages: 2465-2479
  • DOI: 10.1016/j.ajpath.2014.05.012
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A key role for neuropeptide Y in lifespan extension and cancer suppression via dietary restriction (2014)
  • Author(s): Chiba T, Tamashiro Y, Park D, Kusudo T, Fujie R, Komatsu T, Kim SE, Park S, Hayashi H, Mori R, Yamashita H, Chung HY, Shimokawa I.
  • Journal Title: Sci Rep Volume: 4 Issue: 1 Pages: 4517-4517
  • DOI: 10.1038/srep04517
  • NAID: 120006986640
  • Peer Reviewed / Open Access
• [Presentation] Identification of inflammation-related miRNAs in skin wound healing (2014)
  • Author(s): 森 亮一
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Reduced FOXO1 accelerates skin wound healing and attenuates scarring (2014)
  • Author(s): Mori R
  • Organizer: EMBO Conferences, The molecular and cellular basis of regeneration and tissue repair
  • Place of Presentation: Sant Feliu de Guixols (Spain)
  • Year and Date: 2014-09-06 – 2014-09-10
• [Presentation] Pathophysiological role of inflammation-related microRNA in murine skin wound healing (2014)
  • Author(s): Mori R
  • Organizer: FEBS-EMBO Meeting
  • Place of Presentation: Paris (France)
  • Year and Date: 2014-08-30 – 2014-09-04
• [Presentation] 転写因子FoxO1の発現抑制は皮膚創傷治癒を促進し瘢痕形成を減弱する (2014)
  • Author(s): 森 亮一
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島国際会議場（広島県広島市）
  • Year and Date: 2014-04-24 – 2014-04-26
• [Book] 老化の生物学（カロリー制限） (2014)
  • Author(s): 下川 功、森 亮一
  • Total Pages: 20
  • Publisher: 東京化学同人