| Project/Area Number |
25560064
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits
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| Research Institution | Japan Women's University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MISAWA Norihiko 石川県立大学, 生物資源工学研究所, 教授 (30393466)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 女性ホルモン様作用 / 男性ホルモン拮抗作用 / イソフラボンアグリコン / イソフラボン配糖体 / エストロジェンパーシャルアゴニスト / 加熱調理変化 / α―グルコシダーゼ阻害 / エストロジェンレセプター結合能 / Ｐ４５０ |
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| Outline of Final Research Achievements |
We found 8 isoflavone glucosides (compound 1 - 8) in Apios americana Medik, and examined their biological activities. We found compound 2 and 5 possess moderate androgen antagonic activities.
Isoflavone glucosides were reported to be hydrolysed to the corresponding aglycones by b-glucosidase in intestine. Thus, we prepared the aglycones of compounds 1 - 8 (compound 9 - 12) by enzymatic (b-glucosidase) digestion, and examined androgenic and estrogenic activities of compound 9 - 12. As the result, we found compound 10 possess potent estrogen agonistic activity (the potency was about half of genisten).
Next, we checked loss of compound 5 by heat cooking processes (boiled, fryed, steamed, and microwave cooked). Since more than 2/3 of compound 5 was lossed in any cooking processes, isoflavone compounds were proved to be easily broken by heat treatment.
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