Electric device which interfaces heart to regenerated cardiac tissue derived from iPS cells

• Project/Area Number: 25560197
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Osaka University
• Principal Investigator: YAGI Tetsuya 大阪大学, 工学(系)研究科(研究院), 教授 (50183976)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 再生医療 / 組織培養 / iPS細胞 / 電子デバイス / インターフェイス / 神経 / 筋 / 培養心筋細胞 / 神経組織 / 電気刺激 / 集積電子回路 / 神経インターフェイス / 再生医学 / 培養細胞 / 電流刺激 / 集積回路 / フィードバック刺激 / 培養組織 / 心筋細胞 / ペースメーカー / 医用電子デバイス

Outline of Final Research Achievements

To reconstruct the function of disordered excitable organs with implantation of cultured tissues, an electrical device is needed to interface the activities of the host organ to the implanted tissue. We have developed a multi-channel programmable integrated system that can electrically stimulate the tissue during the culture. The system can deliver currents at a high precision and with various spatio-temporal patterns to effectively control the activity of the cultured tissue. The system is compact and can be installed to a microscope, which allows a long term experiment duirng the culture.

Research Products

• [Journal Article] Population response propagation to extrastriate areas evoked by intracortical electrical stimulation in V1 (2016)
  • Author(s): Ｔ.D. Fehervari, T. Yagi
  • Journal Title: Frontiers in Neural Circuits Volume: VOL. 10 Pages: 1-14
  • DOI: 10.3389/fncir.2016.00006
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Voltage-sensitive dye imaging of the visual cortices responding to electrical pulses at different intervals in mice in vivo. (2014)
  • Author(s): Hayashida Y, Takeuchi K, Ishikawa N, Okazaki Y, Tamas F, Tanaka H, Yagi T.
  • Journal Title: Conference Proceedings of IEEE Engineering in Medicine and Biology Society Volume: n.a. Pages: 402-405
  • DOI: 10.1109/embc.2014.6943613
  • Peer Reviewed
• [Journal Article] A multichannel current stimulator chip for spatiotemporal pattern stimulation of neural tissues. (2014)
  • Author(s): Kameda S, Hayashida Y, Tanaka Y, Akita D, Yagi T.
  • Journal Title: Conference Proceedings of IEEE Engineering in Medicine and Biology Society Volume: n.a. Pages: 5011-5015
  • DOI: 10.1109/embc.2014.6944750
  • Peer Reviewed
• [Presentation] Inter-hemispheric neural excitations of visual cortices in response to electrical microstimuli in mice in vivo (2015)
  • Author(s): S. Futami, T.D. Fehervari, T. Yagi, Y. Hayashida
  • Organizer: IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Milano Conference Center (Milan, Italy)
  • Year and Date: 2015-08-25
  • Int'l Joint Research
• [Presentation] Cortical neural excitations in rats in vivo with using a prototype of awireless multi-channel microstimulation system (2015)
  • Author(s): Y. Hayashida, Y. Umehira, K. Takatani, S. Futami, S. Kameda, T. Kamata, A. Ullah. Khan, Y. Takeuchi, M. Imai,and T. Yagi
  • Organizer: IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Milano Conference Center (Milan, Italy)
  • Year and Date: 2015-08-25
  • Int'l Joint Research
• [Presentation] A large scale simulation of excitation propagation in layer 2/3 ofprimary and secondary visual cortices of mice (2015)
  • Author(s): S. Ohtsu, T. Nomura, S. Uno, K. Maeda, Y. Hayashida,and T. Yagi
  • Organizer: IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Milano Conference Center (Milan, Italy)
  • Year and Date: 2015-08-25
  • Int'l Joint Research
• [Presentation] 皮質内微小電気刺激に対する神経興奮範囲の膜電位イメージング (2015)
  • Author(s): 田中雄太, 林田祐樹, 坂田侑弥, 八木哲也
  • Organizer: MEとバイオサイバネティックス研究会
  • Place of Presentation: 玉川大学（東京都・町田市）
  • Year and Date: 2015-03-16 – 2015-03-17
• [Presentation] 膜電位感受性色素を用いたマウス左右大脳皮質間における信号伝達のin-vivoイメージング (2015)
  • Author(s): 二見成俊, 林田祐樹, 八木哲也
  • Organizer: MEとバイオサイバネティックス研究会
  • Place of Presentation: 熊本大学（熊本県・熊本市）
  • Year and Date: 2015-01-22 – 2015-01-23
• [Presentation] マウス大脳視覚野における多点電極刺激に対する時空間神経応答のin-vivoイメージング解析 (2015)
  • Author(s): 高谷光基, 林田祐樹, 竹内浩造, 山口俊平, 岡崎祐香, 八木哲也
  • Organizer: MEとバイオサイバネティックス研究会
  • Place of Presentation: 熊本大学（熊本県・熊本市）
  • Year and Date: 2015-01-22 – 2015-01-23
• [Presentation] Prototyping and Testing of a VLSI Chip for Spatiotemporal Pattern Stimulationin Visual Cortical Prostheses (2014)
  • Author(s): S. Kameda, Y. Hayashida, T. Yagi.
  • Organizer: 計測自動制御学会 Life Engineering Symposium 2014 (LE2014)
  • Place of Presentation: 金沢大学（石川県・金沢市）
  • Year and Date: 2014-09-17 – 2014-09-19
• [Presentation] A Multi-channel Stimulation System with Wireless Communication and Power Transmission for Visual Cortical Prostheses (2014)
  • Author(s): S. Kameda, T. Kamata, Y. Hayashida, M. Imai, Y. Takeushi, T. Yagi.
  • Organizer: Neuroscience 2014 (第37回日本神経科学大会)
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Voltage-sensitive dye imaging of the visual cortices responding to electrical pulses at different intervals in mice in vivo (2014)
  • Author(s): Y. Hayashida, K. Takeuchi, N. Ishikawa, Y. Okazaki, F. Tamas, H. Tanaka, T. Yagi.
  • Organizer: Engineering in Medicine and Biology Society (EMBC), 2014 36th Annual International Conference of the IEEE
  • Place of Presentation: Chicago (The United States of America)
  • Year and Date: 2014-08-26 – 2014-08-30
• [Presentation] A multichannel current stimulator chip for spatiotemporal pattern stimulationof neural tissues (2014)
  • Author(s): S. Kameda, Y. Hayashida, Y. Tanaka, D. Akita, T. Yagi.
  • Organizer: Engineering in Medicine and Biology Society (EMBC), 2014 36th Annual International Conference of the IEEE
  • Place of Presentation: Chicago (The United States of America)
  • Year and Date: 2014-08-26 – 2014-08-30
• [Presentation] 皮質１次視覚野における水平信号伝播の生理学シミュレーション (2014)
  • Author(s): 烏野翔太,津崎圭亮,林田祐樹,八木哲也
  • Organizer: ニューロコンピューティング研究会
  • Place of Presentation: 町田
• [Presentation] 多チャンネル電流パルス刺激チップの試作と評価 (2013)
  • Author(s): 亀田成司, 林田祐樹, 田中宏喜, 秋田大, 八木哲也
  • Organizer: 平成25年電気学会電子・情報・システム部門大会
  • Place of Presentation: 北見
• [Presentation] A Multi-channel Current-Pulse Generator Chip used for High Impedance Stimulating Electrodes (2013)
  • Author(s): Seiji Kameda, Yuki Hayashida, Hiroki Tanaka, Dai Akita and Tetsuya Yagi
  • Organizer: Proceedings of Life Engineering Symposium 201
  • Place of Presentation: Yokohama
• [Presentation] A Multi-Channel Current Stimulator Chip Intended for a Visual Cortical Implant (2013)
  • Author(s): Seiji Kameda, Yuki Hayashida, Hiroki Tanaka, Dai Akita, Atsushi Iwata and Tetsuya Yagi
  • Organizer: The 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Osaka