| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We developed a novel microdevice for transport protein analysis using a cell-sized liposome (extracellular vesicle) which was derived from a human lymphocytic cell with chemical stimulation. A liposome was immobilized on a microhole which was fabricated in a SiN (silicon nitride) diaphragm. Then, the liposome membrane was partially dissolved by surfactant treatment, which permitted the microhole to be covered with a portion of the membrane. The use of FRET (fluorescence resonance energy transfer) enabled detection of fluorescently-labeled glucose (2-NBDG) molecules which were transported by glucose transporter 1 (GLUT1) proteins present in a liposome membrane.
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