Technique for function analysis of membrane transport proteins using cell-derived liposomes
Project/Area Number |
25560204
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Biomedical engineering/Biomaterial science and engineering
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Research Institution | Kyushu Institute of Technology |
Principal Investigator |
YASUDA Takashi 九州工業大学, 大学院生命体工学研究科, 教授 (80270883)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | リポソーム / 膜タンパク質 / トランスポータ / リンパ球細胞 / 微小孔 / マイクロ流路 / 蛍光共鳴エネルギー移動 / トランスポーター / FRET / 界面活性剤 / マイクロマシニング |
Outline of Final Research Achievements |
We developed a novel microdevice for transport protein analysis using a cell-sized liposome (extracellular vesicle) which was derived from a human lymphocytic cell with chemical stimulation. A liposome was immobilized on a microhole which was fabricated in a SiN (silicon nitride) diaphragm. Then, the liposome membrane was partially dissolved by surfactant treatment, which permitted the microhole to be covered with a portion of the membrane. The use of FRET (fluorescence resonance energy transfer) enabled detection of fluorescently-labeled glucose (2-NBDG) molecules which were transported by glucose transporter 1 (GLUT1) proteins present in a liposome membrane.
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Report
(4 results)
Research Products
(7 results)