Tissue engineering on microvessel construction stabilized by pericyte covering

• Project/Area Number: 25560208
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Keio University
• Principal Investigator: SUDO RYO 慶應義塾大学, 理工学部, 准教授 (20407141)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 血管新生 / 組織工学 / 三次元培養 / ペリサイト / マイクロ流体デバイス / 毛細血管ネットワーク / 間葉系幹細胞

Outline of Final Research Achievements

In this study, endothelial cells and mesenchymal stem cells were cocultured in a microfluidic device to construct stable microvasculature covered by pericytes. In particular, we investigated the role of pericytes in the process of vascular formation, and the three-dimensional structure of constructed microvascular networks covered by pericytes. We found culture conditions to promote capillary formation by quantitatively analyzing the interaction between endothelial cells and mesenchymal stem cells, and established a tissue engineering method for construction of stable microvascular networks covered by pericytes. These findings have an important implication for the development of tissue engineering to construct three-dimensional tissues including microvascular networks.

Research Products

• [Journal Article] Solute diffusion through fibrotic tissue formed around protective cage system for implantable devices (2015)
  • Author(s): Gunawan S. Prihandana, Hikaru Ito, Kohei Tanimura, Hiroshi Yagi, Yuki Hori, Orhan Soykan, Ryo Sudo, Norihisa Miki.
  • Journal Title: Journal of Biomedical Materials Research Part B: Applied Biomaterials Volume: Epub ahead of print Issue: 6 Pages: 1180-1187
  • DOI: 10.1002/jbm.b.33298
  • Peer Reviewed
• [Journal Article] Multiscale tissue engineering for liver reconstruction (2014)
  • Author(s): Sudo R
  • Journal Title: Organogenesis Volume: 10 Issue: 2 Pages: 216-224
  • DOI: 10.4161/org.27968
  • Peer Reviewed / Open Access
• [Journal Article] MEMS技術を応用した再生工学 (2014)
  • Author(s): 須藤 亮
  • Journal Title: 日本機械学会誌 Volume: 117 Pages: 24-27
• [Journal Article] The stabilization effect of mesenchymal stem cells on the formation of microvascular networks in a microfluidic device (2013)
  • Author(s): Kyoko Yamamoto, Kohei Tanimura, Yo Mabuchi, Yumi Matsuzaki, Seok Chung, Roger D. Kamm, Mariko Ikeda, Kazuo Tanishita, Ryo Sudo.
  • Journal Title: Journal of Biomechanical Science and Engineering Volume: 8 Pages: 114-128
  • NAID: 130003366817
  • Peer Reviewed
• [Journal Article] Steady and pulsatile shear stress induce differenct three-dimensional endothelial networks through pseudopodium formation (2013)
  • Author(s): Yoshinori Abe, Ryo Sudo, Mariko Ikeda, Kazuo Tanishita
  • Journal Title: Journal of Biorheology Volume: 27 Issue: 1-2 Pages: 38-48
  • DOI: 10.1007/s12573-012-0056-5
  • Peer Reviewed
• [Journal Article] Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse (2013)
  • Author(s): Sekiguchi Y, Masamoto K, Takuwa H, Kawaguchi H, Kanno I, Ito H, Tomita Y, Itoh Y, Suzuki N, Sudo R, Tanishita K
  • Journal Title: Advances in experimental medicine and biology Volume: 789 Pages: 419-25
  • DOI: 10.1007/978-1-4614-7411-1_56
  • ISBN: 9781461472568, 9781461474111
  • Peer Reviewed
• [Journal Article] Endothelial progenitor cells promote directional three-dimensional endothelial network formation by secreting vascular endothelial growth factor. (2013)
  • Author(s): Y. Abe, Y. Ozaki, J. Kasuya, K. Yamamoto, J. Ando, R. Sudo, M. Ikeda and K. Tanishita
  • Journal Title: PLoS ONE Volume: 8(12) Issue: 12 Pages: 1-12
  • DOI: 10.1371/journal.pone.0082085
  • Peer Reviewed
• [Journal Article] 原子間力顕微鏡 (2013)
  • Author(s): 須藤 亮
  • Journal Title: 動脈硬化予防 Volume: 12 Pages: 111-113
• [Journal Article] 原子間力顕微鏡による分子間力の計測 (2013)
  • Author(s): 須藤 亮
  • Journal Title: Vascular Medicine Volume: 9 Pages: 192-196
• [Journal Article] 流れ刺激による血管ネットワーク形成のイメージング解析 (2013)
  • Author(s): 須藤 亮
  • Journal Title: 日本血栓止血学会誌 Volume: 24 Pages: 576-581
  • NAID: 130003393320
• [Presentation] 血管形成開始における血管内皮細胞と間葉系幹細胞の相互作用の影響 (2015)
  • Author(s): 上森寛元、谷村耕平、須藤亮
  • Organizer: 第14回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] ペリサイト付き毛細血管培養モデルからNVUモデル構築への展開 (2015)
  • Author(s): 須藤亮
  • Organizer: Neurovascular Unit研究会2015
  • Place of Presentation: 慶應義塾大学医学部信濃町キャンパス（東京都新宿区）
  • Year and Date: 2015-01-31
  • Invited
• [Presentation] 間葉系幹細胞と血管内皮細胞の相互作用による血管形成の開始 (2015)
  • Author(s): 谷村耕平、上森寛元、須藤亮
  • Organizer: 日本機械学会 第27回バイオエンジニアリング講演会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2015-01-09 – 2015-01-10
• [Presentation] HUVEC-MSC interactions in the initiation of capillary network formation (2014)
  • Author(s): Hiroyuki Uwamori, Kohei Tanimura, and Ryo Sudo
  • Organizer: IEEE Engineering in Medicine & Biology Society (EMBC) Micro and nanotechnology in medicine conference (MNMC) 2014
  • Place of Presentation: Turtle Bay Resort (Oahu, HI, USA)
  • Year and Date: 2014-12-08 – 2014-12-12
• [Presentation] Multiscale liver and vascular tissue engineering (2014)
  • Author(s): Ryo Sudo
  • Organizer: 17th Biomechanics Seminar
  • Place of Presentation: 京都大学再生医科学研究所（京都府京都市）
  • Year and Date: 2014-11-14
  • Invited
• [Presentation] Capillary formation under interstitial flow in a microfluidic device for liver tissue engineering (2014)
  • Author(s): Ryo Sudo, Yoshinori Abe, Saki Menjo, Kazuo Tanishita
  • Organizer: 2014 BMES (Biomedical Engineering Society) annual meeting
  • Place of Presentation: Henry B. Gonzalez Convention Center (San Antonio, TX, USA)
  • Year and Date: 2014-10-22 – 2014-10-25
• [Presentation] 医工薬連携によるOrgans-on-a-chipの開発 (2014)
  • Author(s): 須藤亮
  • Organizer: ４大学ナノ・マイクロファブリケーション・シンポジウム
  • Place of Presentation: 新川崎・創造のもり地区（神奈川県川崎市）
  • Year and Date: 2014-09-16
  • Invited
• [Presentation] マイクロ流体デバイスを用いた細胞及び増殖因子位置制御による毛細血管網再構築の検討 (2014)
  • Author(s): 上森寛元、谷村耕平、須藤亮
  • Organizer: 日本機械学会年次大会
  • Place of Presentation: 東京電機大学（東京都足立区）
  • Year and Date: 2014-09-07 – 2014-09-10
• [Presentation] Reconstruction of microvascular networks in a microfluidic device (2014)
  • Author(s): Ryo Sudo, Hiroyuki Uwamori, Kohei Tanimura, Yoshinori Abe, Kazuo Tanishita
  • Organizer: 4th Japan-Switzerland Workshop on Biomechanics
  • Place of Presentation: Shima Kanko Hotel The Classic (Shima, Mie, Japan)
  • Year and Date: 2014-09-01 – 2014-09-04
  • Invited
• [Presentation] Effect of unidirectional VEGF supply on the formation of capillary networks with pericytes in a microfluidic device (2014)
  • Author(s): Hiroyuki Uwamori, Ryo Sudo
  • Organizer: 7th World Congress of Biomechanics
  • Place of Presentation: John B. Hynes Veterans Memorial Convention Center (Boston, MA, USA)
  • Year and Date: 2014-07-06 – 2014-07-11
• [Presentation] The effect of interstitial flow on the formation of microvascular networks in a microfluidic device (2014)
  • Author(s): Ryo Sudo, Yoshinori Abe, Kohei Tanimura, Hiroyuki Uwamori, Kazuo Tanishita
  • Organizer: The 18th International Vascular Biology Meeting
  • Place of Presentation: Miyakomesse (Kyoto, Japan)
  • Year and Date: 2014-04-14 – 2014-04-17
• [Presentation] 毛細血管ネットワークの再構築における間質流の影響 (2014)
  • Author(s): 谷村 耕平，山本 興子，須藤 亮
  • Organizer: 日本機械学会 第26回 バイオエンジニアリング講演会
  • Place of Presentation: 東北大学片平キャンパス（宮城県仙台市）
• [Presentation] 増殖因子濃度勾配によるHUVEC-MSC共培養における毛細血管網再構築の検討 (2014)
  • Author(s): 上森寛元，谷村 耕平，須藤 亮
  • Organizer: 第13回 日本再生医療学会総会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
• [Presentation] HUVEC-MSC共培養による毛細血管再構築に与える間質流の影響 (2014)
  • Author(s): 谷村 耕平，上森寛元，須藤 亮
  • Organizer: 第13回 日本再生医療学会総会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
• [Presentation] 間質流制御による三次元組織の再生 (2014)
  • Author(s): 須藤 亮
  • Organizer: 第13回 日本再生医療学会総会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
• [Presentation] 間質流制御による3次元血管ネットワークの形成 (2013)
  • Author(s): 阿部順紀，谷下一夫，須藤亮
  • Organizer: 第36回 日本バイオレオロジー学会年会
  • Place of Presentation: 九州大学 西新プラザ（福岡県福岡市）
• [Presentation] Microvessel formation induced with mechanical stimuli (2013)
  • Author(s): Kazuo Tanishita, Ryo Sudo, Yoshinori Abe, Mariko Ikeda
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: 大阪国際会議場（大阪府大阪市）
• [Presentation] Effect of interstitial flow on reconstruction of capillary networks in HUVEC-MSC coculture (2013)
  • Author(s): Kohei Tanimura, Kyoko Yamamoto, Ryo Sudo
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: 大阪国際会議場（大阪府大阪市）
• [Presentation] Reconstruction of capillary networks in HUVEC-MSC coculture cultured in static/flow conditions in a microfluidic platform (2013)
  • Author(s): Kohei Tanimura, Kyoko Yamamoto, Ryo Sudo
  • Organizer: The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)
  • Place of Presentation: Freiburg, Germany
• [Presentation] MEMS技術を利用した血管複合組織の再生
  • Author(s): 須藤 亮
  • Organizer: 第2回イノバイオ研究会セミナー「生体組織再生研究の最前線」
  • Place of Presentation: 東京大学 本郷キャンパス（東京都文京区）
• [Remarks] 慶應義塾大学理工学部システムデザイン工学科須藤研究室ホームページ
  • URL: http://www.sudo.sd.keio.ac.jp
• [Remarks] 慶應義塾大学理工学部システムデザイン工学科須藤研究室ホームページ
  • URL: http://www.sudo.sd.keio.ac.jp