| Project/Area Number |
25560222
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Shinshu University (2014)
Osaka University (2013) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MORISHIMA Keisuke 大阪大学, 工学研究科, 教授 (60359114)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 細胞凍結保存 / インクジェット |
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| Outline of Final Research Achievements |
Cryopreservation is to enable cell stocks to be stored semi-permanently. It is invaluable when dealing with cells with limited life span. We propose a novel cell cryopreservation method without cryoprotectant agent (CPA) by putting cells inside of picoliter droplets using inkjet printing, which inhibits the generation and growth of ice crystals to damages cell membrane and organelles. The viability of the flash freezing was around 20 %, while the viability of the slow-rate freezing without DMSO was almost zero. The result indicates that inkjet-based-flash freezing could be a promising approach for CPA-free cryopreservation.
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