| Project/Area Number |
25560237
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical systems
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| Research Institution | Kobe University |
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Principal Investigator |
YOSHIDA MASARU 神戸大学, 医学(系)研究科(研究院), 准教授 (00419475)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | がん / 診断 / 超臨界 / 質量分析 / バイオマーカー / 超臨界流体 / がん診断 |
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| Outline of Final Research Achievements |
The aim of this study is to establish the new system based on supercritical fluid chromatography mass spectrometry (SFC-MS), and also to construct the system to analyze serum/plasma metabolites by SFC-MS. SFC-MS is useful to analyze hydrophobic molecules such as lipids, but serum and plasma have an abundance of hydrophilic molecules as well as hydrophobic molecules. Therefore, in SFC-MS analysis, we determined the analyzability condition for hydrophilic molecules in addition to hydrophobic molecules, and found the condition to analyze the human serum metabolites by SFC-MS. In addition, we confirmed that SFC-MS is possible to analyze the serum as biological samples and moreover to evaluate the difference between samples. These results indicate that SFC-MS analysis may be applicable to the disease diagnosis system.
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