| Project/Area Number |
25560240
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical systems
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKAGI NORIO 東京薬科大学, 薬学部, 教授 (50318193)
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| Co-Investigator(Renkei-kenkyūsha) |
MARUYAMA KAZUO 帝京大学, 薬学部, 教授 (30130040)
NOMIZU MOTOYOSHI 東京薬科大学, 薬学部, 教授 (00311522)
TAKAHASHI YOKO 東京薬科大学, 薬学部, 助手 (30453806)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 集束超音波 / 血液脳関門 / バブルリポソーム / 核酸・遺伝子デリバリー |
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| Outline of Final Research Achievements |
Here, we tried to develop the nucleic acid - gene delivery system into the brain by the combination of Bubble liposomes (BLs) and high-intensity focused ultrasound (HIFU). First, we examined whether the permeability of the BBB can be enhanced by the combination of BLs and HIFU using Evans blue (EB). As a result, the accumulation of EB in the HIFU-exposed brain was increased over that observed in the non-HIFU-exposed brain. Similarly, the combination of BLs and HIFU allowed antisense oligonucleotides or plasmid DNA to be delivered into the HIFU-exposed brain. Furthermore, polyplex-loaded BLs with HIFU further increased the gene expression in the delivered to the brain by the combination method of BLs and HIFU. Thus, the brain targeting method by the combination of BLs loading therapeutic genes and HIFU together may serves as a useful means for accelerating the permeability of BBB and thereby enabling therapeutic genes to be delivered to the focused brain site in CNS diseases.
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