Intraspinal microstimulation for resturing hand movement in the patient with spinal cord injury

• Project/Area Number: 25560294
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: SEKI Kazuhiko 独立行政法人国立精神・神経医療研究センター, 神経研究所 モデル動物開発研究部, 部長 (00226630)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脊髄刺激 / 霊長類 / 機能再建 / 初期位置 / 脊髄 / 電気刺激 / 脊髄損傷 / 運動 / 上肢

Outline of Final Research Achievements

In this study, first, we developped a method to implant array stimululation electrode in the spinal cord of monkey and confirmed it manufests the exerted muscle force by temporal and spacial summation. Then, we examined the position effect of stimulation result. Proper execution of voluntary movement requires a sensorimotor transformation based on the initial limb state. To test if this transformation could occur at the spinal level, we stimulated the cervical spinal cord of anesthetized monkeys while systematically changing initial posture and examined the modulation of the twitch response induced in the upper limb muscles.Results suggest that excitability in the cervical spinal cord is affected by initial arm posture through spinal reflex pathways. This posture-dependency of spinal motor output could affect voluntary arm movement by adjusting descending motor commands relative to the initial arm posture.

Research Products

• [Journal Article] Internal capsule stroke in the common marmoset (2015)
  • Author(s): Puentes S, Seki K.
  • Journal Title: Neuroscience Volume: 284 Pages: 400-411
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Modulation of spinal motor output by initial arm postures in anesthetized Monkeys. (2015)
  • Author(s): Kazuhiko Seki, Hiroaki Yaguchi, Tomohiko Takei, David Kowalski, Takafumi Suzuki and Kunihiko Mabuchi
  • Journal Title: The Journal of Neuroscience Volume: 35(17) Issue: 17 Pages: 6937-6945
  • DOI: 10.1523/jneurosci.3846-14.2015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 中脳赤核と運動機能―系統発生的観点から―Red Nucleus and Its Motor Function –A Phylogenetic Perspective– (2014)
  • Author(s): 大屋知徹・関和彦
  • Journal Title: Spinal Surgery Volume: 28（3） Pages: 258-263
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Spinal premotor interneurons mediate dynamic and static motor commands for precision grip in monkeys (2013)
  • Author(s): Takei T, Seki K
  • Journal Title: J Neurosci Volume: Vol. 33 Issue: 20 Pages: 8850-60
  • DOI: 10.1523/jneurosci.4032-12.2013
  • Peer Reviewed
• [Journal Article] Synaptic and functional linkages between spinal premotor interneurons and hand- muscle activity during precision grip (2013)
  • Author(s): Takei T, Seki K
  • Journal Title: Front Comput Neurosci Volume: Vol.7, No.40 Pages: 40-40
  • DOI: 10.3389/fncom.2013.00040
  • Peer Reviewed
• [Journal Article] Efficient embryo transfer in the common marmoset monkey (Callithrix jacchus) with a reduced transfer volume : a non-surgical approach with cryo-preserved late-stage embryos (2013)
  • Author(s): Ishibashi H, Motohashi HH, Kumon M, Yamamoto K, Okada H, Okada T, Seki K
  • Journal Title: Biol Reprod Volume: Vol.88, No.5 Issue: 5 Pages: 115-115
  • DOI: 10.1095/biolreprod.113.109165
  • Peer Reviewed
• [Journal Article] Effect of the size of zona pellucida opening on hatching in the common marmoset monkey (Callithrix jacchus) embryo (2013)
  • Author(s): Ishibashi H, Motohashi HH, Kumon M, Yamamoto K, Okada H, Okada T, Seki K
  • Journal Title: Anim Sci J Volume: Vol.84, No.11 Issue: 2 Pages: 740-743
  • DOI: 10.1016/j.repbio.2013.02.002
  • Peer Reviewed
• [Journal Article] Ultrasound-guided non-surgical embryo collection in the common marmoset (2013)
  • Author(s): Ishibashi H, Motohashi HH, Kumon M, Yamamoto K, Okada H, Okada T, Seki K
  • Journal Title: Reprod Biol Volume: Vol.13, No.2 Issue: 11 Pages: 139-144
  • DOI: 10.1111/asj.12115
  • Peer Reviewed
• [Presentation] 運動駆動細胞群の筋シナジー創出機構 (2015)
  • Author(s): 大屋知徹、武井智彦、関和彦
  • Organizer: 平成26年度生理学研究所研究会「行動システム脳科学の新展開」
  • Place of Presentation: 生理学研究所
  • Year and Date: 2015-01-09 – 2015-01-10
• [Presentation] Subcortical control of voluntary movement (2014)
  • Author(s): K. Seki
  • Organizer: 18th Thai Neuroscience Society Conference 2014 and 2 nd CU-NIPS Symposium “Frontier in Neuroscience Research”
  • Place of Presentation: Chulalongkorn University, Bangkok, Thailand
  • Year and Date: 2014-12-22 – 2014-12-23
  • Invited
• [Presentation] Posture dependency of the twitch responses induced by intraspinal microstimulation to the primate spinal cord (2014)
  • Author(s): H. Yaguchi, D. Kowalski, T. Takei and K. Seki
  • Organizer: Society for Neuroscience2014
  • Place of Presentation: Walter E. Washington Convention Center, Washington D.C., U.S.
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] ３次元筋骨格モデルに基づくニホンザル精密把握動作の逆動力学解析, Inverse dynamic analysis of precision grip in the Japanese macaque based on a three-dimensional musculoskeletal model. (2014)
  • Author(s): 荻原直道、斉藤剛、武井智彦、関和彦
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 異なる下行路系から生み出される筋投射構造と筋シナジーへの収斂 (2014)
  • Author(s): 大屋知徹、武井智彦、関和彦
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 赤核の回路と機能における推察 (2014)
  • Author(s): 大屋知徹, 武井智彦, 関和彦
  • Organizer: 第8回Motor Control研究会
  • Place of Presentation: つくば大学
  • Year and Date: 2014-08-07 – 2014-08-09
• [Presentation] Neural mechanisms underlying sensory gating during volitional hand movement. (2013)
  • Author(s): Seki K
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館, 京都
  • Invited
• [Presentation] 霊長類における運動制御と脊髄機能 (2013)
  • Author(s): 関和彦
  • Organizer: 第19回スパインフロンティア
  • Place of Presentation: 鎌倉パークホテル, 鎌倉
  • Invited
• [Presentation] Synaptic distribution patterns of rubromotoeuronal cells onto forelimb muscles for a whole-limb movement in the macaque monkey． (2013)
  • Author(s): Oya T, Takei T, Seki K
  • Organizer: 23rd Annual meeting of the Neural control of Movement
  • Place of Presentation: El San Juan Hotel, Puerto Rico, USA
• [Presentation] Excitatory interneurons that mediate non-reciprocal excitatory reflex in primate spinal cord: their input-output relations and firing pattern during voluntary wrist movement. (2013)
  • Author(s): Kim G, Takei T, Seki K
  • Organizer: IUPS2013
  • Place of Presentation: ICC, Birmingham, UK
• [Presentation] Firing properties of first order INs in the spinal cord of monkey performing wrist flexion- extension task with an instructed delay period. (2013)
  • Author(s): Tomatsu S, Kim G, Takei T, Seki K
  • Organizer: Neuroscience2013
  • Place of Presentation: San Diego Convention Center, San Diego, USA
• [Presentation] Monosynaptic group Ia excitation in first dorsal interossei (FDI) muscles during various manipulation tasks in man. (2013)
  • Author(s): Seki K , Nakajima T, Kim G
  • Organizer: Neuroscience2013
  • Place of Presentation: San Diego Convention Center, San Diego, USA
• [Presentation] Functional specialization of parallel descending motor pathways for prehention, revealed by synaptic linkages of cortical versus rubral systems with forelimb muscles for the macaque monkey. (2013)
  • Author(s): Oya T, Takei T, Seki K
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館, 京都
• [Presentation] A new model of lacunar stroke in the non-human primate. (2013)
  • Author(s): Puentes S, Kaido T, Hanakawa T, Otsuki T, Seki K
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館, 京都
• [Presentation] Rostrocaudal distribution of calbindin D28k positive cells in the ventral horn of the cervical spinal cord in monkeys. (2013)
  • Author(s): Nishimaru H, Kakizaki M, Seki K
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館, 京都
• [Presentation] Contrasting roles of spinal and cortical premotor neurons for a control of grasping. (2013)
  • Author(s): Takei T, Seki K
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館, 京都
• [Presentation] Effect of afferent input to activity of spinal interneuron. (2013)
  • Author(s): Tomatsu S, Kim G, Takei T, Seki K
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館, 京都
• [Book] 「脊髄反射とその下降路制御」Clinical Neuroscience (2013)
  • Author(s): 関和彦
  • Total Pages: 3
  • Publisher: 中外医学社