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Comprehensive analysis for interaction of oxygen binding protein with mitochondrial proteins in skeletal muscle

Research Project

Project/Area Number 25560336
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Sports science
Research InstitutionKanazawa University

Principal Investigator

MASUDA Kazumi  金沢大学, 人間科学系, 教授 (50323283)

Co-Investigator(Renkei-kenkyūsha) TAKAKURA Hisahi  同志社大学, スポーツ健康科学部, 助教 (20631914)
IWANAKA Nobumasa  金沢大学, 人間科学系, 研究員(博士研究員) (80584002)
HASHIMOTO Takeshi  立命館大学, スポーツ健康科学部, 准教授 (70511608)
HAYANO Toshiya  立命館大学, 生命科学部, 教授 (90332303)
HANAI Yoshiteru  名古屋工業大学, 工学研究科, 准教授 (50360730)
KATO Yukio  金沢大学, 薬学系, 教授 (30251440)
SUGIURA Tomoko  金沢大学, 薬学系, 助教 (70542190)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords筋細胞 / 代謝 / ミトコンドリア / ミオグロビン / 相互作用 / 運動 / 呼吸
Outline of Final Research Achievements

Cellular respiration depends upon a coordinated response of the cardiovasculature and metabolism. Myoglobin (Mb), expressing specifically in myocytes, has been found in mitochondria. This unique finding has led us to the hypothesis that mitochondrial Mb may have a direct role for the regulation of mitochondrial respiration. In order to test this idea, we determined protein-protein interaction between Mb and mitochondrial proteins in genetically modified C2C12 cell by proteomic analysis, IP and WB. It was confirmed that Flag-tagged Mb localized in mitochondria in the C2C12 cells that express Flag-Mb stably. Proteomic analysis identified several mitochondrial proteins, which interacted with Mb. These proteins detected in IP samples of Mb-Flag, occupied 24% of entire proteins. IP and WB detected both outer and inner membrane proteins (VDAC and COX-IV) from the mitochondrial fraction. These results suggested the potential interaction of mitochondrial proteins with Mb in skeletal myocytes.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (7 results) (of which Invited: 1 results) Remarks (2 results)

  • [Journal Article] 膜透過処理による骨格筋ミトコンドリア呼吸活性の定量化と異なる筋線維タイプにおける酸素消費速度の比較2014

    • Author(s)
      澤本加那子, 山田達也, 蔭地野稔, 石澤里枝, Hamidie DR Ronald, 新田咲, 増田和実
    • Journal Title

      北陸体育学会紀要

      Volume: 50 Pages: 7-18

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reply to Pancheva, Panchev, and Pancheva.2013

    • Author(s)
      Masuda K, Yamada T, Jue T.
    • Journal Title

      Journal of Applied Physiology

      Volume: 115 Issue: 1 Pages: 151-151

    • DOI

      10.1152/japplphysiol.00449.2013

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] New insights for the factor regulating muscle mitochondrial respiration2014

    • Author(s)
      Yamada T, Jue T and Masuda K
    • Organizer
      Cell Symposia System approach to Metabolic Diseases
    • Place of Presentation
      Chicago, IL, USA
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] 骨格筋ミトコンドリアの呼吸調節機序に関わる新規知見2014

    • Author(s)
      山田達也, 石澤里枝, Hamidie DR Ronald, 増田和実
    • Organizer
      第69回日本体力医学会大会
    • Place of Presentation
      長崎大学, 長崎
    • Year and Date
      2014-09-19 – 2014-09-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 骨格筋ミトコンドリアへの酸素供給に対するミオグロビンの貢献の可能性2013

    • Author(s)
      増田和実
    • Organizer
      第21回日本運動生理学会大会
    • Place of Presentation
      埼玉・東京国際大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Interaction of mitochondrial proteins with myoglobin in C2C12, mouse skeletal muscle cells2013

    • Author(s)
      Yamada T, Iwanaka N, Hashimoto T, Jue T, Masuda K
    • Organizer
      American College of Sports Medicine 60th Annual Meeting
    • Place of Presentation
      Indiana Convention Center, Indianapolis, IN, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] AICAR stimulation increased mitochondrial cd36 presence in C2C12 cells2013

    • Author(s)
      Nitta S, Ishizawa R, Yamada T, Hamidie DR Ronald, Masuda K
    • Organizer
      European College of Sport Science 18th Annual Congress
    • Place of Presentation
      The Institut National d’Educacio Fisica de Catalunya (INEFC)
    • Related Report
      2013 Research-status Report
  • [Presentation] カフェインがミオグロビンタンパク質合成に及ぼす影響2013

    • Author(s)
      岩中伸壮, 石澤里枝, 山田達也, 高倉久志, 橋本健志, 横川拓弥, 増田和実
    • Organizer
      第68回日本体力医学会大会
    • Place of Presentation
      東京・日本教育会館
    • Related Report
      2013 Research-status Report
  • [Presentation] 骨格筋ミトコンドリア呼吸のミオグロビン依存的調節2013

    • Author(s)
      山田達也, 蔭地野稔, 石澤里枝, 増田和実
    • Organizer
      第68回日本体力医学会大会
    • Place of Presentation
      東京・日本教育会館
    • Related Report
      2013 Research-status Report
  • [Remarks] 金沢大学運動生理学・生化学研究室HP(増田研究室)

    • URL

      http://exercisephysiol.com

    • Related Report
      2014 Annual Research Report
  • [Remarks] 金沢大学運動生理学研究室 Website(増田研究室 Website)

    • URL

      http://exercisephysiol.com/

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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