Autophagy is involved in muscle wasting in tumor-bearing cachectic mice.
| Project/Area Number |
25560375
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 悪液質 / オートファジー / 骨格筋 / 萎縮 / 癌 / サイトカイン / 担癌 / 骨格筋萎縮 |
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| Outline of Final Research Achievements |
Cancer cachexia is a highly complex metabolic disorder. Patients exhibit a significant loss of skeletal muscle mass. Recent studies propose the contribution of autophagy-lysosome system to this process. However, its underling mechanisms are still largely unknown. We established an experimental system using human-to-mouse xenotransplantation models of human colorectal cancer to investigate muscle wasting in cahexia. The successfully grew in NOG mice were fixed for immunofluorescence microscopy. An autophagosome marker LC3 was detected as several fine dots in the muscle fibers in control muscle. In tumor-bearing mice, the LC3-dots were significantly increased and often observed as ring or cup-shaped structures, indicating typical autophagosomes. When double-stained with LC3 and p62, p62-dots were largely colocalized with LC3-dots. This study supports the idea that autophagy plays important roles in the muscles wasting in cancer cachexa, and suggests that p62 might mediate this process.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Differing susceptibility to autophagic degradation activity of two LC3-binding proteins: SQSTM1/p62 and TBC1D25/OATL1.2016
Author(s)
Hirano, S., Uemura, T., Annoh, H., Fujita, N., Waguri, S., Itoh, T. and Fukuda, M.
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Journal Title
Autophagy
Volume: 12
Issue: 2
Pages: 312-326
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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