| Project/Area Number |
25560407
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 生合成 / thioviridamide / チオアミド / prodigiosin / 側鎖環化遺伝子 / roseophilin / Streptomyces / 環化遺伝子 / ハロゲナーゼ |
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| Outline of Final Research Achievements |
The genome analysis of a thioviridamide (TVA)-producing organism showed a gene encoding the TVA precursor peptide (tvaA). Heterologous production of TVA in Streptomyces lividans identified the thioviridamide biosynthetic gene cluster containing 12 genes (tvaA - tvaL). Prethioviridamide was extracted from the TVA producer with methanol as a true biosynthetic product, which was converted to TVA by acetone treatment.
In the rph gene cluster of the roseophilin producer, four genes including rphG showed homology to the prodigiosin cyclization gene redG. A redG disruptant of S. coelicolor expressing rphG produced two cyclized prodigiosins, metacycloprodigiosin and a new analogue, propyl-meta-cyclooctylprodiginine, suggesting that the rphG is a prodigiosin cyclization gene with novel regioselectivity.
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