Biomolecule PET based on one-pot/flow 68Ga-DOTA labeling
| Project/Area Number |
25560410
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biomolecular chemistry
|
|---|
| Research Institution | Institute of Physical and Chemical Research |
|---|
Principal Investigator |
Tanaka Katsunori 国立研究開発法人理化学研究所, 田中生体機能合成化学研究室, 准主任研究員 (00403098)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 68Ga-DOTA / アザ電子環状反応 / 理研クリック反応 / アルデヒド / アミノ基 / ポジトロン / ペプチド / PET |
|---|
| Outline of Final Research Achievements |
Convenient and general method for labeling of lysines of biomolecules was established. Azide-containing labels were initially conjugated with the unsaturated ester aldehyde containing the strained acetylenes, by the strain-promoted click reaction. The resulting probes were then treated with the peptides, proteins, antibodies, and even live cell surface in one-pot process, efficiently labeling these biomolecules by azaelectrocyclization (now called as RIKEN click reaction) under the mild conditions. The efficient one-pot procedure enabled the introduction of various labels, e.g., positron emitters, paramagnetic metals, and fluorescences, very rapidly onto the various biomolecules including the sensitive and precious materials which are available with only limited amount. Applicability of biologically important molecules towards diagnosis and therapy will be expanded significantly.
|
Report
(4 results)
Research Products
(118 results)
-
-
-
[Journal Article] In vivo imaging of advanced glycation end products (AGEs) of albumin: first observations of significantly reduced clearance and liver deposition properties in mice2016
Author(s)
Tsutsui A, Ogura A, Tahara T, Nozaki S, Urano S, Hara M, Kojima S, Kurbangalieva A, Onoe H, Watanabe Y, Taniguchi N, Tanaka K
-
Journal Title
Organic and Biomolecular Chemistry
Volume: -
Issue: 24
Pages: 5755-5760
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] Visualizing Trimming Dependence of Biodistribution and Kinetics with Homo- and Heterogeneous N-Glycoclusters on Fluorescent Albumin2016
Author(s)
A. Ogura, T. Tahara, S. Nozaki, K. Morimoto, Y. Kizuka, S. Kitazume, M. Hara, S. Kojima, H. Onoe, A. Kurbangalieva, N. Taniguchi, Y. Watanabe, K. Tanaka
-
Journal Title
Sci. Rep.
Volume: 6
Issue: 1
Pages: 21797-21797
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
[Journal Article] 複合領域研究と国際共同研究2015
Author(s)
田中克典
-
Journal Title
文部科学省科学研究費補助金「新学術領域研究(領域提案型)」天然物ケミカルバイオロジー：分子標的と活性制御ニュースレター
Volume: 11
Pages: 11-11
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
[Journal Article] Facile Preparation of 1,5-Diazacyclooctanes from Unsaturated Imines: Effects of the Hydroxyl Groups on [4+4] Dimerization2014
Author(s)
Tanaka, K., Matsumoto, R., Pradipta, A. R., Kitagawa, Y., Okumura, M. Manabe. Y., Fukase, K.
-
Journal Title
Synlett
Volume: 25
Issue: 07
Pages: 1026-1030
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] A cascading reaction sequence involving ligand-directed azaelectrocyclization and autooxidation-induced fluorescence recovery enables visualization of target proteins on the surfaces of live cells.2014
Author(s)
Tanaka, K., Kitadani, M., Tsutsui, A., Pradipta, AR., Imamaki, R., Kitazume, S., Taniguchi, N., Fukase K.
-
Journal Title
Org Biomol Chem.
Volume: 12
Issue: 9
Pages: 1412-1418
DOI
Related Report
Peer Reviewed
-
[Journal Article] Development of bis-unsaturated ester aldehydes as amino-glue probes: sequential double azaelectrocyclization as a promising strategy for bioconjugation.2013
Author(s)
Tanaka, K., Nakamoto, Y., Siwu, ER., Pradipta, AR., Morimoto, K., Fujiwara, T., Yoshida, S., Hosoya, T., Tamura, Y., Hirai, G., Sodeoka, M., Fukase, K.
-
Journal Title
Org Biomol Chem.,
Volume: 11
Issue: 42
Pages: 7326-7333
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 生体内合成化学治療2015
Author(s)
田中克典
Organizer
理研シンポジウム
Place of Presentation
国立研究開発法人理化学研究所(埼玉県和光市）
Year and Date
2015-06-26
Related Report
Invited
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Sweet Role of Platelet Endothelial Cell Adhesion Molecule (PECAM) in Understanding Angiogenesis2014
Author(s)
Shinobu Kitazume, Rie Imamaki, Ayako Kurimoto, Kazuko Ogawa, Masaki Kato, Yoshiki Yamaguchi, Katsunori Tanaka, Hideharu Ishida, Hiromune Ando, Makoto Kiso, Noritaka Hashii, Nana Kawasaki, Naoyuki Taniguchi
Organizer
SFG-JSCR Joint Meeting 2014
Place of Presentation
Hilton Hawaiian Village Waikiki Beach Resort
Year and Date
2014-11-16
Related Report
-
-
[Presentation] 生体内合成化学治療2014
Author(s)
田中克典
Organizer
早稲田ー理研合同ミニシンポジウム
Place of Presentation
軽井沢早稲田大学セミナーハウス（長野県軽井沢）
Year and Date
2014-10-12
Related Report
Invited
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
