| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
The aim of this study is development of novel fluorescent dye which visualizes structure of tissue even at deep region. We took the advantage of "solvatochromism", the ability to change color depending on the environment of the dye. In this study, we improved imaging techniques to observe deeper region of tissue in animals and explore and design novel fluorescent dyes. Regarding improvement of imaging techniques, (1) establishment of simple clearing protocol for tissue observation, (2) development of transmissive liquid crystal devices correcting optical aberrations, (3) improvement of high-speed multiphoton microscopy using a multipoint-scanning method, and (4) establishment of non-invasive skin imaging in an anesthetized living mouse were carried out. Finally, by intradermal injection of novel developed fluorescent dyes, it was succeeded to visualize three-dimensional structure of skin in a living mouse.
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