Design of Detection System for Histone Modification Patterns Using Split-Proteins
Project/Area Number |
25560412
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Chemical biology
|
Research Institution | Tohoku University |
Principal Investigator |
SAKAMOTO Seiji 東北大学, 多元物質科学研究所, 助教 (30335228)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | エピジェネティクス / ヒストンテール / 翻訳後修飾 / 分割型タンパク質 / GFP / ルシフェラーゼ / バイオプローブ / ヒストン / 分割型緑色蛍光タンパク質 |
Outline of Final Research Achievements |
It has been suggested that the posttranslational modifications on histone tails modulate the chromatin structure and function. Therefore, the development of a highly sensitive and specific detection system for histone modification patterns will provide new insights into intricate epigenetic mechanisms. In this study, we attempted to construct a fluorescent detection system for histone modification patterns by utilizing split-GFP. In the strategy to generate split-GFP that reassembles in response to a particular histone modification, chromatin related domains were conjugated to split-GFP fragments. We envisioned that the reassembly and functional recovery of split-GFP would be promoted only in the presence of histone tail molecules possessing particular modification patterns.
|
Report
(3 results)
Research Products
(15 results)
-
-
-
-
-
-
-
-
-
-
[Presentation] 第24回バイオ高分子シンポジウム2014
Author(s)
坂本清志, 寺内美香, AnnaHugo, 荒木 保幸, 和田健彦
Organizer
第24回バイオ高分子シンポジウム
Place of Presentation
東京
Year and Date
2014-07-24 – 2014-07-25
Related Report
-
-
-
-
-