| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
It has been suggested that the posttranslational modifications on histone tails modulate the chromatin structure and function. Therefore, the development of a highly sensitive and specific detection system for histone modification patterns will provide new insights into intricate epigenetic mechanisms. In this study, we attempted to construct a fluorescent detection system for histone modification patterns by utilizing split-GFP. In the strategy to generate split-GFP that reassembles in response to a particular histone modification, chromatin related domains were conjugated to split-GFP fragments. We envisioned that the reassembly and functional recovery of split-GFP would be promoted only in the presence of histone tail molecules possessing particular modification patterns.
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