Screen the chemicals that efficiently induce mitophagy

• Project/Area Number: 25560414
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemical biology
• Research Institution: Niigata University
• Principal Investigator: KANKI Tomotake 新潟大学, 医歯学系, 教授 (50398088)
• Co-Investigator(Kenkyū-buntansha): YAMASHITA Tomohiro 九州大学, 薬学研究科(研究院), 特任助教 (30645635)
• Co-Investigator(Renkei-kenkyūsha): YAMASHITA Shun-ichi 新潟大学, 医歯学系, 特任助教 (30529095)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ミトコンドリア / オートファジー / マイトファジー

Outline of Final Research Achievements

Mitophagy is a process that degrades cytoplasmic proteins and organelles. Recent studies revealed that autophagy selectively degrade mitochondria and this process is called mitochondria autophagy or mitophagy. Mitophagy is conserved within almost all eukaryote. Recently, it is known that mitophagy selectively degrade damaged or dysfunctional mitochondria, in another word, mitophagy contribute to maintain cellular mitochondrial homeostasis. In this study, we aim to identify the chemicals that efficiently induce mitophagy and applied the chemicals for clinical use. We identified several candidate chemicals which can induce mitophagy.

Research Products

• [Journal Article] Mitophagy is primarily due to alternative autophagy and requires MAPK1 and MAPK14 signaling pathway. (2015)
  • Author(s): Hirota, Y.,Yamashita, S., Kurihara, Y., Jin, X., Aihara, M., Saigusa, T., Kang, D., and Kanki, T.
  • Journal Title: Autophagy Volume: 11 Issue: 2 Pages: 332-43
  • DOI: 10.1080/15548627.2015.1023047
  • Peer Reviewed / Open Access
• [Journal Article] Mitophagy in yeast: Molecular mechanisms and physiological role. (2015)
  • Author(s): Kanki T, Furukawa K, Yamashita SI.
  • Journal Title: Biochim Biophys Acta. Volume: 印刷中 Issue: 10 Pages: 2756-2765
  • DOI: 10.1016/j.bbamcr.2015.01.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Tor and the Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32 in yeast. (2014)
  • Author(s): Aihara M, Jin X, Kurihara Y, Yoshida Y, Matsushima Y, Oku M, Hirota Y, Saigusa T, Aoki Y, Uchiumi T, Yamamoto T, Sakai Y, Kang D, Kanki T.
  • Journal Title: Journal of Cell Science Volume: 127 Pages: 3184-96
  • DOI: 10.1242/jcs.153254
  • Peer Reviewed / Open Access
• [Journal Article] Assays for autophagy II: Mitochondrial autophagy. (2014)
  • Author(s): Kanki T, Okamoto K.
  • Journal Title: Methods Mol Biol. Volume: 1163 Pages: 165-173
  • DOI: 10.1007/978-1-4939-0799-1_11
  • ISBN: 9781493907984, 9781493907991
  • Peer Reviewed
• [Journal Article] Casein kinase 2 is essential for mitophagy. (2013)
  • Author(s): Kanki T., Kurihara Y., Jin X., Goda T., Ono Y., Aihara M., Hirota Y., Saigusa T., Aoki Y., Uchiumi T., Kang D.
  • Journal Title: EMBO reports Volume: 14巻 Issue: 9 Pages: 788-794
  • DOI: 10.1038/embor.2013.114
  • Peer Reviewed
• [Journal Article] Mutation and functional analysis of ABCC2/multidrug resistance protein 2 in a Japanese patient with Dubin-Johnson syndrome (2013)
  • Author(s): Uchiumi T, Tanamachi H, Kuchiwaki K, Kajita M, Matsumoto S, Yagi M, Kanki T, Kang D
  • Journal Title: Hepatol Res Volume: 43(5) Issue: 5 Pages: 569-75
  • DOI: 10.1111/j.1872-034x.2012.01103.x
  • NAID: 10031183491
  • Peer Reviewed
• [Presentation] The signaling pathway regulating mitochondria autophagy (2015)
  • Author(s): Tomotake Kanki
  • Organizer: 2nd International Symposium on Protein Modifications in Pathogenic Dysregulation of Signaling
  • Place of Presentation: 東大医科研
  • Year and Date: 2015-01-23 – 2015-01-24
  • Invited
• [Presentation] Signaling pathways regulating mitophagy in yeast (2014)
  • Author(s): Tomotake Kanki
  • Organizer: 2014 Northeastern Asian Symposium on Autophagy
  • Place of Presentation: 韓国ソウル
  • Year and Date: 2014-12-18 – 2014-12-21
  • Invited
• [Book] Yeast Protocols, Third Edition (2014)
  • Author(s): Kanki T, Okamoto K
  • Publisher: Humana Press
• [Book] AUTOPHAGY, CANCER, OTHER PATHOLOGIES, INFLAMMATION, IMMUNITY, INFECTION AND AGING, VOLUME 4 (2014)
  • Author(s): Kukrihara U, Kanki T.
  • Publisher: Elsevier Inc.
• [Remarks] 新潟大学医学部機能制御学分野
  • URL: http://www.med.niigata-u.ac.jp/mit/