optogenetic analsysis of single neurons in vivo
| Project/Area Number |
25560424
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Basic / Social brain science
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| Research Institution | Nagoya University |
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Principal Investigator |
TAKAGI Shin 名古屋大学, 理学(系)研究科(研究院), 准教授 (90171420)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 光遺伝学 / 運動制御 / Ｃ． ｅｌｅｇａｎｓ / IR-LEGO / 線虫C. elegans / 摂食運動 |
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| Outline of Final Research Achievements |
To understand how a neural circuit regulates a certain behavior, we are focusing on the simple pharyngeal nervous system of C. elegans constituted by 20 neurons of 14 types. We have manipulated the activity of subsets of neurons in free-moving worms by using optogenetic tools. We have found that silencing of several types neurons including M2 neurons decreased the frequency of pumping, sometimes resulting in complete arrest of pumping. Our preliminary study also showed that activation of a certain neuron suppressed pumping. Although a previous cell ablation study showed that MCs are the only neurons in the pharynx controlling the frequency of the pharyngeal pumping movement, our results suggest that multiple cell types are involved in the regulation of pumping frequency.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] A blue-shifted light-driven proton pump for neural silencing2013
Author(s)
Sudo, Y., Okazaki, A., Ono, H., Yagasaki, J., Sugo, S., Kamiya, M., Reissig, L., Inoue, K., Ihara, K., Kandori, H., Takagi, S., Hayashi, S.
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Journal Title
J. Biol. Chem.
Volume: 288
Issue: 28
Pages: 20624-20632
DOI
Related Report
Peer Reviewed
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