Quantitative localization of membrane molecules at nano-scale spatial resolution
| Project/Area Number |
25560425
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Basic / Social brain science
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| Research Institution | University of Fukui (2014)
Nagoya University (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 電子顕微鏡 / 生体分子 / 分子局在 / 分子定量 / 凍結割断レプリカ標識法 / 分子形態 / 絶対量 / 超微構造 / 凍結割断レプリカ法 / 分子絶対量 |
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| Outline of Final Research Achievements |
Each of the biological phenomenon or functions of our body is generated by the interaction among molecules at subcellular and cellular levels. Thus, in order to deepen our knowledge about underlying mechanisms for the biological phenomenon and to develop new therapeutic approaches for diseases, it is important to uncover quantity (expression density) of individual bio-molecules at nano-scale spatial resolution. However, it is not possible to obtain such information by conventional molecular localization techniques. Here in this research project, I introduced several optimizations in SDS-digested freeze-fracture replica labeling and revealed localization of several ion channels in neuronal plasma membrane.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Coassembly and Coupling of SK2 Channels and mGlu5 Receptors2014
Author(s)
Garcia-Negredo G, Soto D, Llorente J, Morato X, Galenkamp KMO, Gomez-Soler M, Fernandez-Dunas V, Watanabe M, Adelman JP, Shigemoto R, Fukazawa Y, Lujan R, Ciruela F
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Journal Title
J Neurosci
Volume: 33
Pages: 14793-14802
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Distinct cerebellar engrams in short-term and long-term motor learning2014
Author(s)
Wang W, Nakadate K, Masugi-Tokita M (W .W., K.N., M.M.-T., and F.S. contributed equally to this work), Shutoh F, Aziz W, Tarusawa E, Lorincz A, Molnár E, Kesaf S, Li YQ, Fukazawa Y, Nagao S, Shigemoto R
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Journal Title
Proc Natl Acad Sci USA
Volume: 111(1)
Issue: 1
DOI
Related Report
Peer Reviewed
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