SERS-DNA sequencer

• Project/Area Number: 25600049
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nanobioscience
• Research Institution: Osaka University (2013, 2015); Waseda University (2014)
• Principal Investigator: Yamaguchi Yoshinori 大阪大学, 工学(系)研究科(研究院), 教授 (20386634)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: DNAシーケンサ / 表面増強ラマン散乱 / マイクロチップ / 普及型DNAシーケンサ / ナノ剣山増強ラマンデバイス / ナノ剣山 / DNAシーケンサー / 普及型DNAシーケンサー

Outline of Final Research Achievements

In this study, as the first step for DNA sequencer based on surface enhanced Raman spectroscopy (SERS), we invented the SERS active substrate that enable to detect the surface enhanced Raman signal from single molecule. This result and invention of the SERS active nano-needle mountain is the big step for the SERS based DNA sequencer.

Research Products

• [Int'l Joint Research] 華東理工大学/理学研究科(中国)
• [Int'l Joint Research] 上海理工大学/光電信息研究院(中国)
• [Journal Article] Capillary electrophoresis of RNA in hydroxyethylcellulose polymer with various molecular weights. (2016)
  • Author(s): Zhenqing Li, Chenchen Liu, Dawei Zhang, Yoshinori Yamaguchi
  • Journal Title: Journal of Chromatography B Volume: 1011 Pages: 114-120
  • DOI: 10.1016/j.jchromb.2015.12.057
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Electrophoresis of Periodontal Pathogens in Poly (ethyleneoxide) Solutions with Uncoated Capillary (2015)
  • Author(s): Zhenqing Li, Ronglin Yang, Q Wang, Dawei Zhang, Songlin Zhuang, Yoshinori Yamaguchi
  • Journal Title: Analytical Biochemistry Volume: 471 Pages: 70-72
  • DOI: 10.1016/j.ab.2014.11.002
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Polyethylene Oxide (PEO) and Polyethylene Glycol (PEG) Polymer Sieving Matrix for RNA Capillary Electrophoresis (2015)
  • Author(s): Yoshinori Yamaguchi, Zhenqing Li, Xifang Zhu, Chenchen Liu, Dawei Zhang, Xiaoming Dou
  • Journal Title: PLoS One Volume: 10(6) Issue: 5 Pages: e0123406-e0123406
  • DOI: 10.1371/journal.pone.0123406
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Analysis of small interfering RNA by capillary electrophoresis in hydroxyethylcellulose solutions (2015)
  • Author(s): Chenchen Liu, Yoshinori Yamaguchi, Xifang Zhu, Zhenqing Li, Yi Ni, Xaoming Dou
  • Journal Title: Electrophoresis Volume: 36 Issue: 14 Pages: 1651-1657
  • DOI: 10.1002/elps.201500018
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Time-lapse Raman imaging of osteoblast differentiation (2015)
  • Author(s): Aya Hashimoto, Yoshinori Yamaguchi, Liang-da Chiu, Chiaki Morimoto, Katsumasa Fujita, Masahide Takedachi, Satoshi Kawata, Shinya Murakami, Eiichi Tamiya
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 12529-12529
  • DOI: 10.1038/srep12529
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Activation energy of the Belousov– Zhabotinsky reaction in a gel with Fe(bpy)3 catalyst (2014)
  • Author(s): Yusuke Hara, Yoshinori Yamaguchi, Hiroyuki Mayama, Kenji Fujimoto
  • Journal Title: Chemistry Letters Volume: 43 Pages: 673-675
  • NAID: 130004868109
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Quantification of Periodontal Pathogens Cell Counts by Capillary Electrophoresis (2014)
  • Author(s): Zhenqing Li, Shaoxiong Chen, Chenchen Liu, Dawei Zhang, Xiaoming Dou, Yoshinori Yamaguchi
  • Journal Title: Journal of Chromatography A Volume: 1361 Pages: 286-290
  • DOI: 10.1016/j.chroma.2014.07.100
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Determination and quantification of Escherichia coli by capillary electrophoresis (2014)
  • Author(s): Zhenqing Li, De Li, Dawei Zhang, Yoshinori Yamaguchi
  • Journal Title: Analyst Volume: 139 Issue: 23 Pages: 6113-6117
  • DOI: 10.1039/c4an01649a
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Electromigration behavior of nucleic acids in capillary electrophoresis under pulsed-field conditions (2014)
  • Author(s): Zhenqing Li, Chenchen Liu, Xiaoming Dou, Yi Ni, Jiaxuan Wang, Yoshinori Yamaguchi
  • Journal Title: Journal of Chromatography A Volume: 1331 Pages: 100-107
  • Peer Reviewed
• [Journal Article] Capillary electrophoresis of wide range DNA fragments in mixed solution of hydroxyethyl cellulose (2014)
  • Author(s): Zhenqing Li, Chenchen Liu, Yoshinori Yamaguchi, Yi Ni, Qingxiang You, Xiaoming Dou
  • Journal Title: Analytical Methods Volume: 6 Issue: 8 Pages: 2473-2477
  • DOI: 10.1039/c3ay41965g
  • Peer Reviewed
• [Journal Article] In situ Raman imaging of osteoblastic mineralization (2014)
  • Author(s): Aya Hashimoto, Liang-da Chiu, Keigo Sawada, Katsumasa Fujita, Masahide Takedachi, Yoshinori Yamaguchi, Satoshi Kawata, Shinya Murakami, Eiichi Tamiya
  • Journal Title: Journal of Raman Spectroscopy Volume: 45 Issue: 2 Pages: 157-161
  • DOI: 10.1002/jrs.4438
  • Peer Reviewed
• [Journal Article] Switching the Belousov-Zhabotinsky reaction with a Strong-Acid-Free Gel (2014)
  • Author(s): Y. Hara, Y. Yamaguchi, H. Mayama
  • Journal Title: J. Phys. Chem. B Volume: 118 Issue: 2 Pages: 634-638
  • DOI: 10.1021/jp410915p
  • Peer Reviewed