Development of molecular manipulation in biological cells with surface plasmon resonance based optical trapping
Project/Area Number |
25600052
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Nanobioscience
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Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
Hosokawa Chie 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (60435766)
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Co-Investigator(Renkei-kenkyūsha) |
TAWA Keiko 関西学院大学, 理工学部, 教授 (80344109)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 光ピンセット / ナノバイオ / 表面プラズモン共鳴 / 神経細胞 / 表面プラズモン |
Outline of Final Research Achievements |
We demonstrate surface plasmon resonance based optical trapping using a plasmonic chip toward realizing effective manipulation of molecules on the surface of neurons. Surface-plasmon-enhanced optical trapping was evaluated by the fluorescence analysis of nanoparticles suspended in water and neural cell adhesion molecules (NCAMs) labeled with quantum dots (Q-dots) on rat hippocampal neurons. The motion of nanoparticles in water and the molecular dynamics of NCAMs on neuronal cells cultured on a plasmonic chip were constrained at the laser focus more effectively than those on a glass substrate because of the surface plasmon resonance effect.
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Report
(5 results)
Research Products
(67 results)
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[Presentation] Bright fluorescence imaging of cells cultured on the plasmonic dish2014
Author(s)
K. Tawa, T. Fujita, C. Yasui, K. Kiyosue, C. Hosokawa, C. Sasagawa, H. Aota, N. Kakinuma, M. Oike, J. Nishii
Organizer
The 248th ACS National Meeting
Place of Presentation
San Francisco, USA
Year and Date
2014-08-12
Related Report
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