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Automated Embryonic Body Culture System by using Membrane Micromachining

Research Project

Project/Area Number 25600054
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Nano/Microsystems
Research InstitutionThe University of Tokyo

Principal Investigator

IKEUCHI Masashi  東京大学, 先端科学技術研究センター, 助教 (90377820)

Co-Investigator(Kenkyū-buntansha) MIYAMOTO Yoshitaka  独立行政法人国立成育医療研究センター, 生殖・細胞医療研究部, 研究員 (20425705)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords胚様体 / 再生医療 / PASCL / 圧力駆動 / 自動培養 / マイクロ・ナノデバイス / 再生医学 / 幹細胞 / MEMS
Outline of Final Research Achievements

We have proposed and developed a “Pneumatically Actuated Spheroids Culture Chip (PASCL)”. PASCL has the ability to produce and culture EBs of defined size highly in parallel, and has a unique mechanism to collect a targeted EB selectively from the arrays of EBs. In detail, PASCL consists of three parts; top layer with fluid channels, a PDMS membrane and bottom layer with pneumatic actuation lines. The pneumatic actuation lines have square openings on the topside. By applying negative pressure in the pneumatic actuation lines, the PDMS membrane set between the top and the bottom layer is pulled into the openings to form concave microwells where EBs are produced. After EB formation and differentiation, a targeted EB is popped up into the fluid channel by applying atmos-pheric pressure in the pneumatic actuation line, and collected by flow. The prototype has microwells arrayed 10x10 in 1 cm2. By using human iPS, EB formation, differentiation and sorting were verified.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (17 results)

All 2015 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (10 results) (of which Invited: 1 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] 膜マイクロ・ナノファブリケーション技術による組織再生デバイス2014

    • Author(s)
      池内真志
    • Journal Title

      BIOINDUSTRY

      Volume: 31 Pages: 37-44

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Three-Dimensional in vitro Hepatic Constructs Formed Using Combinatorial Tapered Stencil for Cluster Culture (TASCL) Device2014

    • Author(s)
      Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, Shuji Hayashi
    • Journal Title

      Cell Medicine

      Volume: -

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 新概念マイクロマシンから攻める再生医療2013

    • Author(s)
      池内真志,生田幸士
    • Journal Title

      バイオマテリアル

      Volume: 31 Pages: 165-170

    • Related Report
      2013 Research-status Report
  • [Presentation] 胚様体大量培養デバイスTASCLの量産化2015

    • Author(s)
      池内真志, 豊田悠司, 生田幸士, 林衆治
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 胚様体の均一・大量生産を実現する培養デバイス"TASCL"の量産化2014

    • Author(s)
      池内真志, 豊田悠司, 生田幸士, 林衆治
    • Organizer
      第41回日本臓器保存生物医学会学術集会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府豊中市)
    • Year and Date
      2014-11-28 – 2014-11-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] TASCLを用いた胚様体の大量生産と長期培養技術2014

    • Author(s)
      池内真志, 豊田悠司, 生田幸士, 林衆治
    • Organizer
      第36回日本バイオマテリアル学会大会
    • Place of Presentation
      タワーホール船堀
    • Year and Date
      2014-11-17 – 2014-11-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] ポリマー薄膜を用いた3次元マイクロデバイスの作製と評価2014

    • Author(s)
      池内真志
    • Organizer
      第6回マイクロ・ナノ工学シンポジウム
    • Place of Presentation
      くにびきメッセ(島根県松江市)
    • Year and Date
      2014-10-20 – 2014-10-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Three-dimensional Microforming of Polymer Membrane for Medical and Biological Applications2014

    • Author(s)
      M. Ikeuchi
    • Organizer
      IUMRS-ICA 2014
    • Place of Presentation
      福岡大学(福岡市)
    • Year and Date
      2014-08-24 – 2014-08-30
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 再生医療のための全自動胚様体大量培養デバイスの開発2014

    • Author(s)
      安川あかね, 池内真志, 西島拓弥, 井上佳則, 生田幸士
    • Organizer
      第53回日本生体医工学会
    • Place of Presentation
      仙台国際センター(仙台市)
    • Year and Date
      2014-06-24 – 2014-06-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 再生医療研究のための全自動胚様体培養チップの開発2014

    • Author(s)
      安川あかね, 西島拓弥, 池内真志, 生田幸士
    • Organizer
      日本機械学会ロボティクス・メカトロニクス講演会2014
    • Place of Presentation
      富山市総合体育館(富山市)
    • Year and Date
      2014-05-25 – 2014-05-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Co-cultured Cluster Formation and Self-organization of Cells in Hydrophilic Tapered Microwell Array2014

    • Author(s)
      M. Ikeuchi, S. Hayashi, K. Ikuta
    • Organizer
      IEEE NEMS 2014
    • Place of Presentation
      Hawaii (USA)
    • Year and Date
      2014-04-13 – 2014-04-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Integrated micro culture device for fully automated closed culture experiment of embryonic body2014

    • Author(s)
      Akane Yasukawa, Takuya Nishijima, Masashi Ikeuchi, Koji Ikuta
    • Organizer
      IEEE MEMS 2014
    • Place of Presentation
      San Francisco, U.S.A
    • Related Report
      2013 Research-status Report
  • [Presentation] Fabrication and Differentiation of Embryonic Bodies in “PASCL (Pneumatically Actuated Spheroid Culturing Lab-on-a-chip)”2013

    • Author(s)
      Akane Yasukawa, Takuya Nishijima, Masashi Ikeuchi, Koji Ikuta
    • Organizer
      IEEE EMBC 2013
    • Place of Presentation
      Osaka
    • Related Report
      2013 Research-status Report
  • [Remarks] 東京大学 生田研究室

    • URL

      http://www.micro.rcast.u-tokyo.ac.jp/

    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞培養装置および細胞培養方法2014

    • Inventor(s)
      池内真志, 林衆治, 豊田悠司
    • Industrial Property Rights Holder
      池内真志
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-11-14
    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞培養装置およびこれを備える細胞培養試験観察システム2014

    • Inventor(s)
      池内真志, 生田幸士, 安川あかね
    • Industrial Property Rights Holder
      東京大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-07-24
    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞培養用シートおよびその製造方法2014

    • Inventor(s)
      豊田悠司, 池内真志, 林衆治
    • Industrial Property Rights Holder
      日東電工ほか
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-06-16
    • Related Report
      2014 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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