| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The purpose of this study was to develop a microfluidic system that provides a three-dimensional cell culture environment by the reconstituted vascularized networks. It enables long-term tissue culture on a chip for morphogenesis studies. We have developed an on-chip angiogenesis assay, optimized spheroid culture condition to be injected to the chip, and realized vascularized network with a spheroid embedded. Further, the network was found perfusable by injecting microspheres and fluorescent dyes.
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