| Project/Area Number |
25610117
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological physics/Chemical physics/Soft matter physics
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 人工細胞モデル / リポソーム |
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| Outline of Final Research Achievements |
This project aims to construct a cell model by using giant unilamellar vesicle (GUV) with extracted live cell components. During the project, we developed the novel method for introducing micro objects (~1um) into live cells, by using electrofusion with GUVs. Briefly, dispersed cells and GUVs were placed into an electrofusion chamber, and were exposed to an AC field to align cells-GUVs, and were also exposed DC pulse to induce transient electrofusion. The processed cell reached confluence with entrapping specified sizes of fluorescent beads. The transfer efficiency was evaluated by using fluorescence microscopy and flow cytometry. Delivering magnetic beads, DNA origamis, plasmids were also implemented. Introduction of artificial objects into live cells is an important topic in biotechnology. We expect that our novel method will be used for elucidation of cell mechanisms and creation of artificial cell which consists of cellular components.
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