| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Outline of Final Research Achievements |
We present a chemomechanical network model of molecular motor kinesin that is systematically developed on the basis of experimental observations, and demonstrate that it can qualitatively reproduce available all the experimental results of motor dynamics. The analysis of the local excess fluxes shows that an ATP-concentration dependence of the dominant forward stepping cycle and (2) supports a gated rear head mechanism where the forward step is controlled by ATP hydrolysis and the resulting ADP-bound state of the rear head at saturating ATP levels. The network picture suggests that for saturating ATP concentration, the energy from ATP hydrolysis is used to concentrate the chemical transition flux on the network to a force-generating state that can produce the power stroke. At low ATP levels, the energy is used to avoid states that are fragile against backward load because the leading head is weakly bound to microtubule.
|
