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Computerized model building of brain neuronal network

Research Project

Project/Area Number 25610126
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biological physics/Chemical physics/Soft matter physics
Research InstitutionTokai University

Principal Investigator

Mizutani Ryuta  東海大学, 工学部, 教授 (70272482)

Co-Investigator(Renkei-kenkyūsha) TAKEKOSHI Susumu  東海大学, 医学部, 教授 (70216878)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords神経回路 / マイクロCT / マイクロトモグラフィ
Outline of Final Research Achievements

Brain is composed of a huge number of neurons, which constitute neuronal circuits. The first step to analyzing such brain circuits is to trace neuronal networks to build three-dimensional models of their complicated structures. In this study, we have developed methods for building three-dimensional wire models by tracing neuronal networks. We evaluated a number of algorithms reported for tracing wire-form structures in two-dimensional images. Some of those methods were proven to be effective in the computerized building of neuronal network model. A part of this study has been published as a feature article of the September 2015 issue of Microscopy Today, the official publication of the Microscopy Society of America.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (33 results)

All 2016 2015 2014 2013 Other

All Journal Article (16 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 15 results,  Open Access: 10 results,  Acknowledgement Compliant: 6 results) Presentation (12 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (5 results)

  • [Journal Article] A method for estimating spatial resolution of real image in the Fourier domain.2016

    • Author(s)
      Mizutani R, Saiga R, Takekoshi S, Inomoto C, Nakamura N, Itokawa M et al.
    • Journal Title

      J Microsc.

      Volume: 261 Issue: 1 Pages: 57-66

    • DOI

      10.1111/jmi.12315

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recent progress of hard x-ray imaging microscopy and microtomography at BL37XU of SPring-82016

    • Author(s)
      Yoshio Suzuki, Akihisa Takeuchi, Yasuko Terada, Kentaro Uesugi, and Ryuta Mizutani
    • Journal Title

      AIP Conf. Proc.

      Volume: 1696 Pages: 020013-020013

    • DOI

      10.1063/1.4937507

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Three-dimensional structure of brain tissue at submicrometer resolution.2016

    • Author(s)
      Rino Saiga, Ryuta Mizutani, Chie Inomoto, Susumu Takekoshi, Naoya Nakamura, Akio Tsuboi, Motoki Osawa, Makoto Arai, Kenichi Oshima, Masanari Itokawa, Kentaro Uesugi, Akihisa Takeuchi, Yasuko Terada, and Yoshio Suzuki
    • Journal Title

      AIP Conf. Proc.

      Volume: 1696 Pages: 020004-020004

    • DOI

      10.1063/1.4937498

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Three-Dimensional Neuronal Structure of Human Cerebral Cortex Determined by Synchrotron-Radiation Microtomography.2015

    • Author(s)
      Saiga R, Takekoshi S, Inomoto C, Nakamura N, Tsuboi A, Osawa M, Arai M, Oshima K, Itokawa M, Uesugi K, Takeuchi A, Terada Y, Suzuki Y and Mizutani R.
    • Journal Title

      Microsc. Microanal

      Volume: 21 Issue: S3 Pages: 919-920

    • DOI

      10.1017/s1431927615005395

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] X-ray tomographic microscopy of Drosophila brain network and skeletonized model building in the three-dimensional image2015

    • Author(s)
      Ryuta Mizutani, Rino Saiga, Kentaro Uesugi, Akihisa Takeuchi, Yasuko Terada, and Yoshio Suzuki
    • Journal Title

      Microsc. Microanal.

      Volume: 21(S3) Issue: S3 Pages: 917-918

    • DOI

      10.1017/s1431927615005383

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Scanning Brain Networks with Micro-CT2015

    • Author(s)
      Ryuta Mizutani, Rino Saiga, Susumu Takekoshi, Makoto Arai, Akihisa Takeuchi, and Yoshio Suzuki
    • Journal Title

      Microscopy Today

      Volume: 23 Issue: 5 Pages: 12-17

    • DOI

      10.1017/s1551929515000784

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT)2015

    • Author(s)
      Masato Ohtsuka, Hiromi Miura, Keiji Mochida, Michiko Hirose, Ayumi Hasegawa, Atsuo Ogura, Ryuta Mizutani, Minoru Kimura, Ayako Isotani, Masahito Ikawa, Masahiro Sato and Channabasavaiah B Gurumurthy
    • Journal Title

      BMC Genomics

      Volume: 16 Pages: 274-274

    • NAID

      120006957412

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT)2015

    • Author(s)
      Ohtsuka M., Miura H., Mochida K., Hirose M., Hasegawa A., Ogura A., Mizutani R., Kimura M., Isotani A., Ikawa M., Sato M. and Gurumurthy C. B.
    • Journal Title

      BMC Genomics

      Volume: in press

    • NAID

      120006957412

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] SPring-8放射光による神経回路ネットワークの解析2014

    • Author(s)
      水谷隆太,雑賀里乃,竹内晃久,上杉健太朗,寺田靖子,鈴木芳生
    • Journal Title

      顕微鏡

      Volume: 49(3) Pages: 222-225

    • NAID

      130007701737

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Structural biology of DNA (6-4) photoproducts formed by ultraviolet radiation and interactions with their binding proteins2014

    • Author(s)
      Yokoyama, H. Mizutani, R.
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 15 Issue: 11 Pages: 20321-20338

    • DOI

      10.3390/ijms151120321

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Three-dimensional analysis of neuronal circuits of the human brain by X-ray microtomography2014

    • Author(s)
      Ryuta Mizutani, Rino Saiga, Akihisa Takeuchi, Kentaro Uesugi and Yoshio Suzuki
    • Journal Title

      SPring-8 Information

      Volume: 19 Pages: 225-229

    • Related Report
      2014 Research-status Report
    • Open Access / Acknowledgement Compliant
  • [Journal Article] Spatiotemporal development of soaked protein crystal2014

    • Author(s)
      Ryuta Mizutani, Yusuke Shimizu, Rino Saiga, Go Ueno, Yuki Nakamura, Akihisa Takeuchi, Kentaro Uesugi, and Yoshio Suzuki
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 5731-5731

    • DOI

      10.1038/srep05731

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structure of a double-stranded DNA (6–4) photoproduct in complex with the 64M-5 antibody Fab.2013

    • Author(s)
      Hideshi Yokoyama, Ryuta Mizutani, Yoshinori Satow
    • Journal Title

      Acta Crystallographica D

      Volume: 69 Issue: 4 Pages: 504-512

    • DOI

      10.1107/s0907444912050007

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] X-ray microtomographic visualization of Escherichia coli by metalloprotein overexpression2013

    • Author(s)
      Ryuta Mizutani, Keisuke Taguchi, Masato Ohtsuka, Minoru Kimura, Akihisa Takeuchi, Kentaro Uesugi, and Yoshio Suzuki
    • Journal Title

      J. Synchrotron Radiation

      Volume: 20 Issue: 4 Pages: 581-586

    • DOI

      10.1107/s0909049513008467

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] X線マイクロトモグラフィ法によるヒト大脳神経回路の決定2013

    • Author(s)
      水谷隆太,雑賀里乃
    • Journal Title

      放射光

      Volume: 26 Pages: 268-275

    • NAID

      10031191828

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional network of Drosophila brain hemisphere2013

    • Author(s)
      Ryuta Mizutani, Rino Saiga, Akihisa Takeuchi, Kentaro Uesugi and Yoshio Suzuki
    • Journal Title

      J. Struct. Biol.

      Volume: 184 Issue: 2 Pages: 271-279

    • DOI

      10.1016/j.jsb.2013.08.012

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒト大脳皮質の神経回路の三次元解析2015

    • Author(s)
      藤巻知央,菊池亜弥,蓮見真子,竹腰進,井野元智恵,中村直哉,坪井秋男,大澤資樹,新井誠,大島健一,糸川昌成,上杉健太朗,竹内晃久,寺田靖子,鈴木芳生,雑賀里乃,水谷隆太
    • Organizer
      第88回日本生化学会大会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] X-ray Tomographic Microscopy of Drosophila Brain Network and Skeletonized Model Building in the Three-Dimensional Image2015

    • Author(s)
      Ryuta Mizutani, Rino Saiga, Kentaro Uesugi, Akihisa Takeuchi, Yasuko Terada and Yoshio Suzuki
    • Organizer
      Microscopy & Microanalysis 2015 Meeting
    • Place of Presentation
      Oregon Convention Center (Portland, USA)
    • Year and Date
      2015-08-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Three-Dimensional Neuronal Structure of Human Cerebral Cortex Determined by Synchrotron-Radiation Microtomography2015

    • Author(s)
      Rino Saiga, Susumu Takekoshi, Chie Inomoto, Naoya Nakamura, Akio Tsuboi, Motoki Osawa, Makoto Arai, Kenichi Oshima, Masanari Itokawa, Kentaro Uesugi, Akihisa Takeuchi, Yasuko Terada, Yoshio Suzuki and Ryuta Mizutani
    • Organizer
      Microscopy & Microanalysis 2015 Meeting
    • Place of Presentation
      Oregon Convention Center (Portland, USA)
    • Year and Date
      2015-08-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] X-ray microtomographic visualization of microorganisms by metalloprotein overexpression2014

    • Author(s)
      Ryuta Mizutani, Akihisa Takeuchi, Kentaro Uesugi, Yoshio Suzuki.
    • Organizer
      The 12th International Conference on X-Ray Microscopy 2014
    • Place of Presentation
      Melbourne Convention and Exhibition Centre (Melbourne, Australia)
    • Year and Date
      2014-10-26 – 2014-10-31
    • Related Report
      2014 Research-status Report
  • [Presentation] Three-dimensional structure of the fruit-fly brain network2014

    • Author(s)
      Rino Saiga, Ryuta Mizutani, Akihisa Takeuchi, Kentaro Uesugi, Yoshio Suzuki
    • Organizer
      The 12th International Conference on X-Ray Microscopy 2014
    • Place of Presentation
      Melbourne Convention and Exhibition Centre (Melbourne, Australia)
    • Year and Date
      2014-10-26 – 2014-10-31
    • Related Report
      2014 Research-status Report
  • [Presentation] Recent progress of imaging microscopy and microtomography at BL37XU of SPring-82014

    • Author(s)
      Yoshio Suzuki, Akihisa Takeuchi, Yasuko Terada, Kentaro Uesugi, and Ryuta Mizutani
    • Organizer
      The 12th International Conference on X-Ray Microscopy 2014
    • Place of Presentation
      Melbourne Convention and Exhibition Centre (Melbourne, Australia)
    • Year and Date
      2014-10-26 – 2014-10-31
    • Related Report
      2014 Research-status Report
  • [Presentation] Building human brain network in electron density map determined by X-ray microtomography2014

    • Author(s)
      Rino Saiga, Susumu Takekoshi, Naoya Nakamura, Akihisa Takeuchi, Kentaro Uesugi, Yoshio Suzuki, and Ryuta Mizutani
    • Organizer
      23rd Congress and General Assembly of the International Union of Crystallography
    • Place of Presentation
      Palais des congres de Montreal (Montreal, Canada)
    • Year and Date
      2014-08-05 – 2014-08-12
    • Related Report
      2014 Research-status Report
  • [Presentation] Visualizing soaking process of protein crystal2014

    • Author(s)
      Ryuta Mizutani, Yusuke Shimizu, Rino Saiga, Go Ueno, Yuki Nakamura, Akihisa Takeuchi, Kentaro Uesugi, and Yoshio Suzuki
    • Organizer
      23rd Congress and General Assembly of the International Union of Crystallography
    • Place of Presentation
      Palais des congres de Montreal (Montreal, Canada)
    • Year and Date
      2014-08-05 – 2014-08-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 広視野高分解能硬X線結像顕微鏡によるマイクロCT2014

    • Author(s)
      鈴木芳生、寺田靖子、竹内晃久、上杉健太朗、田村繁治、水谷隆太
    • Organizer
      第27回日本放射光学会年会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] X 線マイクロトモグラフィ法によるヒト大脳神経回路の解析2014

    • Author(s)
      水谷隆太
    • Organizer
      SPRUC拡大研究会・SPring-8利用ワークショップ
    • Place of Presentation
      高輝度光科学研究センター
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] X線マイクロトモグラフィ法によるヒト脳組織の三次元構造解析2013

    • Author(s)
      雑賀里乃、水谷隆太、竹腰進、井野元智恵、中村直哉、大澤資樹、新井誠、大島健一、糸川昌成、竹内晃久、上杉健太朗、鈴木芳生
    • Organizer
      第74回応用物理学会秋季学術講演会
    • Place of Presentation
      同志社大学京田辺校
    • Related Report
      2013 Research-status Report
  • [Presentation] X線マイクロトモグラフィ法によるヒト大脳皮質の三次元構造解析2013

    • Author(s)
      水谷隆太、雑賀里乃、竹腰進、井野元智恵、中村直哉、大澤資樹、新井誠、大島健一、糸川昌成、竹内晃久、上杉健太朗、鈴木芳生
    • Organizer
      SPring-8 シンポジウム2013
    • Place of Presentation
      京都大学宇治おうばくプラザ
    • Related Report
      2013 Research-status Report
  • [Remarks] Mizutani Lab, Tokai Univ

    • URL

      http://www.el.u-tokai.ac.jp/ryuta/

    • Related Report
      2015 Annual Research Report
  • [Remarks] Mizutani Laboratory, Dept of Applied Biochemistry

    • URL

      http://www.el.u-tokai.ac.jp/ryuta/

    • Related Report
      2014 Research-status Report
  • [Remarks] SPATIOTEMPORAL DEVELOPMENT; NATIVE

    • URL

      http://www.rcsb.org/pdb/explore.do?structureId=3WPJ

    • Related Report
      2014 Research-status Report
  • [Remarks] SPATIOTEMPORAL DEVELOPMENT; 750 SEC

    • URL

      http://www.rcsb.org/pdb/explore.do?structureId=3wpk

    • Related Report
      2014 Research-status Report
  • [Remarks] Mizutani Laboratory

    • URL

      http://www.el.u-tokai.ac.jp/ryuta/

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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