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Study on deep biosphere ecosystem in oceanic crust by "single-cell" chemical speciation

Research Project

Project/Area Number 25610164
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Geochemistry/Cosmochemistry
Research InstitutionEhime University (2015)
University of Shizuoka (2013-2014)

Principal Investigator

Mitsunobu Satoshi  愛媛大学, 農学部, 准教授 (70537951)

Co-Investigator(Kenkyū-buntansha) SHIRAISHI Fumito  広島大学, 大学院理学研究科, 助教 (30626908)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsシングルセル / スペシエーション / 微生物 / 金属 / シングルセル分析 / 鉄酸化バクテリア / 玄武岩 / パイライト / X線顕微鏡
Outline of Final Research Achievements

It is much difficult to investigate a deep biosphere microbial ecosystem in oceanic crust due to their low activities and difficulty in the analysis. Hence, there is little finding even on general microbial metabolic process of the deep biosphere. In this study, we have developed a new method to analyze chemical species of metals and organic materials at single-cell level by coupling two advanced techniques, scanning transmission X-ray microscopy and fluorescence in situ hybridization.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (14 results)

All 2016 2015 2014 2013

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] Direct Detection of Fe(II) in Extracellular Polymeric Substances (EPS) at the Mineral-Microbe Interface in Bacterial Pyrite Leaching2016

    • Author(s)
      Mitsunobu S., Zhu M., Takeichi Y., Ohigashi T., Suga H., Jinno M., Makita H., Sakata M., Ono K., Mase K., Takahashi Y.
    • Journal Title

      Microbes and Environments

      Volume: 31 Issue: 1 Pages: 63-69

    • DOI

      10.1264/jsme2.ME15137

    • NAID

      130005138797

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nano-scale identification of extracellular organic substances at microbe-mineral interface by scanning transmission X-ray microscopy (STXM)2015

    • Author(s)
      Mitsunobu S, Zhu M, Takeichi Y, Ohigashi T, Suga H, Makita H, Sakata M, Ono K, Mase K, Takahashi Y
    • Journal Title

      Chemistry Letters

      Volume: 44 Pages: 91-93

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Presence of oxygen and aerobic communities from sea floor to basement in deep-sea sediments2015

    • Author(s)
      D’Hondt, S., Inagaki, F., Zarikian, C.A., Yamaguchi, Y.T., et al.
    • Journal Title

      Nature Geoscience

      Volume: 8 Issue: 4 Pages: 299-304

    • DOI

      10.1038/ngeo2387

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Arsenic attenuation in geothermal streamwater coupled with biogenic arsenic(III) oxidation2013

    • Author(s)
      Mitsunobu S., Hamamura N., Kataoka T., Shiraishi F.
    • Journal Title

      Applied Geochemistry

      Volume: 35 Pages: 154-160

    • NAID

      130004594046

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Behavior of antimony(V) during the transformation of ferrihydrite and its environmental implications2013

    • Author(s)
      Mitsunobu S., Muramatsu C., Watanabe K., Sakata M.
    • Journal Title

      Environmental Science & Technology

      Volume: 47 Pages: 9660-9667

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effectiveness of sulfur and boron isotopes in aerosols as tracers of emissions from coal burning in Asian continent.2013

    • Author(s)
      Sataka M., Ishikawa T., Mitsunobu S.
    • Journal Title

      Atmospheric Environment

      Volume: 67 Pages: 296-303

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A new method to observe in situ microbe-metal-mineral interaction by micro-XAFS-FISH technique2013

    • Author(s)
      Mitsunobu S., Shiraishi F.
    • Journal Title

      SPring-8 Research Frontiers

      Volume: 2013 Pages: 102-103

    • Related Report
      2013 Research-status Report
  • [Presentation] Nanoscale identification of organic substances at microbe-mineral interface in bacterial leaching of pyrite by STXM2015

    • Author(s)
      Mitsunobu, S., Zhu, M., Takeichi, Y., Ohigashi, T, Suga, H., Sakata, M., Ono, K., Takahashi, Y.
    • Organizer
      International Conference on the Biogeochemistry of Trace Elements 2015
    • Place of Presentation
      (Fukuoka convention center, Fukuoka
    • Year and Date
      2015-07-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 走査型透過X線顕微鏡の微生物-金属-鉱物相互作用解明への応用2014

    • Author(s)
      光延聖, 朱鳴, 坂田昌弘, 高橋嘉夫, 武市泰男, 小野寛太
    • Organizer
      2014年度日本地球化学会年会
    • Place of Presentation
      富山大学
    • Year and Date
      2014-09-16 – 2014-09-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 走査型透過X線顕微鏡(STXM) の微生物-鉱物相互作用解明への応用2014

    • Author(s)
      光延聖, 朱鳴, 高橋嘉夫, 武市泰男, 小野寛太
    • Organizer
      2014年度日本地球惑星科学連合大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-04-28 – 2014-05-02
    • Related Report
      2014 Research-status Report
  • [Presentation] マイクロXAFS-FISH法による微生物-金属-鉱物相互作用の直接観察2013

    • Author(s)
      光延 聖,白石史人
    • Organizer
      日本地球惑星科学連合2013年会
    • Place of Presentation
      千葉幕張メッセ
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Coupled m-XAFS-FISH technique for direct observation of the microbe-metal-mineral interaction2013

    • Author(s)
      Mitsunobu S, Shiraishi F.
    • Organizer
      Goldschmidt2013
    • Place of Presentation
      フィレンツェ国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] X線吸収分光法を駆使したアンチモンやヒ素などの地球表層での挙動に関する研究2013

    • Author(s)
      光延 聖
    • Organizer
      日本地球化学会2013年会
    • Place of Presentation
      筑波大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 微生物のヒ素酸化反応によって促進される堆積物へのヒ素固定―スペシエーションと機能遺伝子解析による新たなアプローチ2013

    • Author(s)
      光延 聖,濱村奈津子,片岡剛文,白石史人,坂田昌弘
    • Organizer
      日本地球化学会2013年会
    • Place of Presentation
      筑波大学
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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