| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
In this study, novel artificial metalloenzymes have been developed using dicopper copper monooxygenase tyrosinase and metal-binding protein TM1459 from hyperthermophiles. The crystal structures of the deoxy-, met-, and oxy-forms of tyrosinase from Aspergillus oryzae and its mutants have been determined, and their spectroscopic and magnetic characteristics as well as the catalytic activity have been explored in detail. On the basis of those information, artificial dicopper enzymes have been developed for the catalytic phenol oxidation reactions. Furthermore, novel metalloenzymes containing several transition-metal ions such as Zn, Cu, Ni, Co, Fe, Mn, Cr, V, Ru, Os have been developed by using a simple metal-binding protein from Thermotoga maritima, and their crustal structures as well as the spectroscopic, magnetic properties and catalytic activity have been examined in detail.
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