Creation of new time measurement-chemiluminescent reaction and prototyping of compact analyzer
| Project/Area Number |
25620103
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Analytical chemistry
|
|---|
| Research Institution | Ibaraki University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 時間計測 / 化学発光 / 時計反応 / 自己触媒反応 / iTem-CL / 微量分析 / 簡易分析 / 極限計測 |
|---|
| Outline of Final Research Achievements |
In chlorophyll chemiluminescence system, a specific inductive time delay phenomenon of saccharide maltose was found, and the time measurement - chemiluminescence analysis (iTem-CL method) of 0.1μM maltose was established. Moreover, by the kinetic analysis of reaction mechanism for autocatalytic reactions, it was proved that iTem-CL method is a constant sensitivity or "infinite sensitivity" method. For the prototype of compact analyzer, when using the existing chemiluminescence analyzer (Gene Light S-200 made in Microtec Nition ), that even without the improvement of the hard aspect of the apparatus body is available was found. As a basis, the detailed examination of factors such as temperature, mixture, the signal detection was performed. The iTem-CL method was a simple and easy analytical method for clinical sample (maltose in an intravenous feeding agent) and the environmental sample (Ru(III) ion of river water), and extensive application is expected in future.
|
Report
(3 results)
Research Products
(2 results)
