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Construction of fluorescence immunochromatography technique using a long persistent phosphor nanoparticles

Research Project

Project/Area Number 25620110
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Analytical chemistry
Research InstitutionNagoya University

Principal Investigator

TAKI Masayasu  名古屋大学, その他の研究科, 准教授 (70378850)

Project Period (FY) 2013-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords長残光蛍光体 / ナノ粒子化 / 表面修飾 / ナノ粒子 / 葉酸 / 抗体
Research Abstract

The immunochromatographic assay is a simple and rapid test, which provides a result without the need for specialized and costly equipment. Although it is known that a fluorescence immunochromatographic assay is high sensitive for detection of the sample as compared with a traditional colorimetric method, this is lacking in convenience of the use because of the necessary of a fluorescence detector. Herein, I focused on a long persistent ceramics (LPC), which shows intense long-lived afterglow luminescence when irradiated with UV-vis light, and addressed the development of new phosphorescence immunochromatographic techniques with the long persistent ceramics. Ball-milling was used to prepare the nanoparticles of ceramics with long persistent property. The synthetic method to introduce biomolecules such as folic acid and antibody on the surface of the nanoparticles has been established.

Report

(2 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (3 results)

All 2014 2013

All Presentation (3 results)

  • [Presentation] 細胞表面タンパク質への特異的な結合特性を有する長残光蛍光体ナノ粒子の開発2014

    • Author(s)
      谷ロ暢子・ 多喜正泰・ 山本行男
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 長残光蛍光体ナノ粒子を用いた光バイオイメージング2013

    • Author(s)
      多喜正泰・ 西真弓・ 石田昭人・田部勢津久
    • Organizer
      第54回日本組織細胞化学会
    • Place of Presentation
      航空会館
    • Year and Date
      2013-09-27
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 長残光蛍光体ナノ粒子を用いた無励起光in vitroおよびin vivoイメージング2013

    • Author(s)
      多喜正泰・ 谷ロ暢子・ 西真弓・ 篠田達昭・ 上田純平・ 石田昭人・田部勢津久・ 山本行男
    • Organizer
      第8回日本分子イメージング学会
    • Place of Presentation
      横浜赤レンガ倉庫
    • Related Report
      2013 Annual Research Report 2013 Final Research Report

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Published: 2014-07-25   Modified: 2016-11-25  

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