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Development of the real-time ultrasensitive detection method for the chirality change of single DNA molecules in aqueous solutions

Research Project

Project/Area Number 25620113
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Analytical chemistry
Research InstitutionOsaka University

Principal Investigator

TSUKAHARA Satoshi  大阪大学, 理学(系)研究科(研究院), 教授 (50207338)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords蛍光検出円二色性 / キラリティ / 生体関連物質 / タンパク質
Outline of Final Research Achievements

Fluorescence detected circular dichroism (FDCD) is one of the detection methods of the chirality of substances with a high sensitivity. In the present study, FDCD spectra were investigated with a conventional circular dichroism (CD) spectrophotometer.
In 2013, the FDCD spectra of some proteins in aqueous solutions were measured, but they contained artificial signals significantly. In 2014, it was clarified that the intensity of the artificial signals depended on the position of the detector of the CD spectrophotometer. From these results, the detector was placed at the position where the minimum artificial signals were obtained. The FDCD spectra of amino acids labeled with a fluorescent dye were measured, and the FDCD spectra of mirror image were obtained for L- and D-amino acids. Furthermore, intrinsic FDCD spectra of a protein labeled with a fluorescent dye in aqueous solutions were successfully obtained.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (14 results)

All 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (8 results) (of which Invited: 2 results) Remarks (2 results)

  • [Journal Article] In Situ Microscope Measurements of the Formation of Nanometer-Sized Gold Particles at the Dodecane/Water Interface through the Direct Reduction of Aqueous etrachloroaurate(III) by Hydrophobic dl-α-Tocopherol in the Presence of 1,10-Phenanthroline2014

    • Author(s)
      Satoshi TSUKAHARA, Rintaro KOBAYASHI and Terufumi FUJIWARA
    • Journal Title

      Solvent Extraction Research and Development, Japan

      Volume: 21 Pages: 119-127

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Spectroscopic and Microscopic Study on the Extraction Behavior of a Probe Dye, Neutral Red, by Aerosol OT in the Isooctane/Water System2013

    • Author(s)
      Yuya TOYOKAWA, Satoshi TSUKAHARA and Terufumi FUJIWARA
    • Journal Title

      Solvent Extraction Research and Development, Japan

      Volume: 20 Pages: 29-38

    • NAID

      130005056953

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] TSUKAHARA, Satoshi, "Microscope Measurements of Lateral Diffusion of Fluorescent Rhodamine B at Toluene/Water Interface by Total Internal Reflection-Fluorescence Recovery after Photobleaching2013

    • Author(s)
      SHINOMORI, Naoki
    • Journal Title

      Chem. Lett

      Volume: 42 Issue: 4 Pages: 444-446

    • DOI

      10.1246/cl.121223

    • NAID

      10031162202

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Surface tension determination through measurements of resonance oscillation of a small surface using dielectric force by a localized alternating current electric field2013

    • Author(s)
      TSUKAHARA, Satoshi; TSURUTA, Tsuyoshi; FUJIWARA, Terufumi
    • Journal Title

      Analyst

      Volume: 138 Issue: 7 Pages: 2110-2117

    • DOI

      10.1039/c3an36260d

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Surface Tension Determination through Microscope Measurements of Resonance Oscillation of Small Liquid Surface Using Periodic External Forces2014

    • Author(s)
      Satoshi Tsukahara
    • Organizer
      1st Asian Symposium on Analytical Sciences
    • Place of Presentation
      広島大学(東広島市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 水溶液と界面を形成する4-シアノ-4’-ペンチルビフェニル液晶のアンカリングエネルギー測定2014

    • Author(s)
      諏訪 雅頼,中塚 由加里,塚原 聡
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学(東広島市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 液液界面における界面活性剤単分子層の相転移挙動の in situ 顕微蛍光測定2014

    • Author(s)
      歳實 萌,塚原 聡
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学(東広島市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 液液界面微小集合体が示す極微な光吸収量の超高感度分光測定と解析2014

    • Author(s)
      八木 真二,篠森 直樹,塚原 聡
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学(東広島市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 液液界面における金ナノ粒子の会合ダイナミクスの顕微測定2013

    • Author(s)
      立本 絵里,塚原 聡
    • Organizer
      第32回溶媒抽出討論会
    • Place of Presentation
      名古屋大学ES総合館(名古屋市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 液液界面における長鎖アルコール単分子膜の相転移挙動のin situ顕微蛍光測定2013

    • Author(s)
      歳實 萌,塚原 聡
    • Organizer
      第32回溶媒抽出討論会
    • Place of Presentation
      名古屋大学ES総合館(名古屋市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 液液界面で特異的に起こる現象のin situ顕微測定2013

    • Author(s)
      塚原 聡
    • Organizer
      日本分析化学会第62年会
    • Place of Presentation
      近畿大学(東大阪市)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 蛍光検出円二色性(FDCD)測定法を用いたタンパク質のキラリティの高感度検出の検討2013

    • Author(s)
      加藤 諒,片山和也,塚原 聡
    • Organizer
      日本分析化学会第62年会
    • Place of Presentation
      近畿大学(東大阪市)
    • Related Report
      2013 Research-status Report
  • [Remarks] 大阪大学大学院理学研究科化学専攻分析化学研究室

    • URL

      http://www.chem.sci.osaka-u.ac.jp/lab/tsukahara/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 大阪大学大学院理学研究科化学専攻分析化学研究室

    • URL

      http://www.chem.sci.osaka-u.ac.jp/lab/tsukahara/

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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