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Chemical analysis using a single cell trapped by vortex beam as a reactor in organic phase

Research Project

Project/Area Number 25620114
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Analytical chemistry
Research InstitutionOkayama University

Principal Investigator

KANETA Takashi  岡山大学, 自然科学研究科, 教授 (20243909)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsレーザートラッピング / 単一細胞計測 / 光渦 / 水滴 / 油滴 / 融合 / 化学計測
Outline of Final Research Achievements

The present study aimed to develop a novel technique for the analysis of a single cell using laser trapping. A laser trapping technique is useful for manipulating a small object dispersed in a medium with refractive index higher than that of the object. However, it is difficult to manipulate a water droplet in an organic solvent since water has the refractive index lower than that of the organic solvent. To solve this problem, we utilized a vortex beam which has a doughnut-shaped profile. Using the vortex beam, the method for trapping, manipulation, and fusion of water droplets in the organic solvent, manipulation was developed.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (14 results)

All 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results)

  • [Journal Article] Speciation of arsenic in a thermoacidophilic iron-oxidizing archaeon, Acidianus brierleyi, and its culture medium by inductively coupled plasma-optical emission spectroscopy combined with flow injection pretreatment using an anion-exchange mini-column2014

    • Author(s)
      Naoki Higashidani, Takashi Kaneta, Nobuyuki Takeyasu, Shoji Motomizu, Naoko Okibe, Keiko Sasaki
    • Journal Title

      Talanta

      Volume: 122 Pages: 240-245

    • DOI

      10.1016/j.talanta.2014.01.057

    • NAID

      120005425633

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Discrimination of glycoproteins via two-color laser-induced fluorescence detection coupled with postcolumn derivatization in capillary electrophoresis2013

    • Author(s)
      Ayumi Tabara, Takashi Kaneta
    • Journal Title

      Electrophoresis

      Volume: 34 Issue: 16 Pages: 2316-2322

    • DOI

      10.1002/elps.201300149

    • NAID

      120005311971

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Method development for the determination of arsenic by sequential injection/anodic stripping voltammetry using long-lasting gold-modified screen-printed carbon electrode2013

    • Author(s)
      Eakkasit Punrat, Suchada Chuanuwatanakul, Takashi Kaneta, Shoji Motomizu, Orawon Chailapakul
    • Journal Title

      Talanta

      Volume: 116 Pages: 1018-1025

    • DOI

      10.1016/j.talanta.2013.08.030

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Fusion of oil droplets in a microfluidic device using optical tweezers2015

    • Author(s)
      Manami Mitsunobu, Sakurako Kobayashi, Nobuyuki Takeyasu, Takashi Kaneta
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2015
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 光ピンセット法を用いたマイクロ流体デバイス内での油滴融合2015

    • Author(s)
      光延愛美, 金田隆
    • Organizer
      2015年日本化学会中国四国支部大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-11-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 単一細胞計測のための光渦を用いた油相中水滴の捕捉2015

    • Author(s)
      島田雄飛, 金田隆, 武安伸幸
    • Organizer
      2015年日本化学会中国四国支部大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-11-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光渦ビームによる油相中水滴の捕捉と単一細胞計測への応用2015

    • Author(s)
      島田雄飛,武安伸幸,金田隆
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      九州大学
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Single cell analysis using laser trapping2015

    • Author(s)
      Takashi Kaneta
    • Organizer
      2015 BK21Plus Symposium on HRD Center for Creative Convergence Chemical Science & The 2nd SKKU-OU Joint Symposium on Advanced Chemistry
    • Place of Presentation
      Vivaldi Park & Ski Resort, Hongcheon. Korea
    • Year and Date
      2015-02-12
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] レーザートラッピングによる油滴融合における界面活性剤の影響2014

    • Author(s)
      光延 愛美,小林 桜子,武安 伸幸,金田 隆
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学
    • Year and Date
      2014-09-17
    • Related Report
      2014 Research-status Report
  • [Presentation] キャピラリー電気泳動法によるグアニル酸ゲルと芳香族化合物の結合定数測定2013

    • Author(s)
      金田 隆,山口 佳織,武安 伸幸
    • Organizer
      第73回分析化学討論会
    • Place of Presentation
      北海道大学函館キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] Speciation of Arsenic by ICP-OES Coupled with Flow Injection Pretreatment Using an Anion Exchange Mini-Column2013

    • Author(s)
      Naoki Higashidani, Takashi Kaneta, Nobuyuki Takeyasu, Shoji Motomizu, Naoko Okibe, Keiko Sasaki
    • Organizer
      Asianalysis XII
    • Place of Presentation
      九州大学馬出キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] ガラス基板上に作製した脂質支持膜の全反射蛍光観察2013

    • Author(s)
      牧 朋美,武安 伸幸,金田 隆
    • Organizer
      日本分析化学会第62年会
    • Place of Presentation
      近畿大学東大阪キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] バイオ分析化学の新展開を目指して2013

    • Author(s)
      金田 隆
    • Organizer
      第29回分析化学緑陰セミナー
    • Place of Presentation
      旅亭 雪の屋,旭川
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Discrimination of Glycoproteins in a Protein Mixture by Two-Color Laser-Induced Fluorescence in Capillary Electrophoresis2013

    • Author(s)
      Takashi Kaneta
    • Organizer
      The 1st Japan-Korea Symposium and Autumn Seminar on Advanced Chemistry
    • Place of Presentation
      Okayama University
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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