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Biosensing of pain-sensation-related chemicals and in-vivo fluorescent imaging

Research Project

Project/Area Number 25620115
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Analytical chemistry
Research InstitutionKyushu University

Principal Investigator

NAKANO Koji  九州大学, 工学(系)研究科(研究院), 准教授 (10180324)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsバイオセンサー / バニロイド / 受容体 / 電位チャンネル / 合成ペプチド / 蛍光共鳴エネルギー移動 / カプサイシン / 自己組織化膜 / 電位チャネル
Outline of Final Research Achievements

By mimicking the transient receptor potential vanilloid receptor 1, two-types of potential artificial receptors that recognize and bind various vanilloid molecules were newly synthesized. The amino-acid sequences of the peptide hosts were as follows: LAMGWTNMLY HHHHHH (VB-His) and YSEILFFVQS-HHHHHH-SLAMGWTNML (VB2-His). Fluorometry spectra for VB-His obtained in the presence of vanillin and related compounds revealed that the host could recognize vanillin to give spectral responses. VB2-His, on the other hand, gave spectrum almost similar to that of the intended guest molecule, capsaicin, which made further applications difficult. Although various fluorophores were examined as alternatives, none of them gave positive outcomes ever. As a consequence one of the initial plan of capsaicin detection for applications to chemical pain sensing was unsuccessful. However, by taking advantage of quartz-crystal microbalance measurements, VB-His was successfully applied to vanillin sensing.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (18 results)

All 2015 2014 2013

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Acknowledgement Compliant: 2 results,  Open Access: 6 results) Presentation (7 results)

  • [Journal Article] Synthesis and Self-Assembly of His-tag Hybrid of Substrate-Binging Short Domain in Transient Receptor Potential Vanilloid Type 1 for Vanillin Sensing Application2015

    • Author(s)
      Koji Nakano, Shingo Hirata, Jun Horiuchi, Ryoichi Ishimatsu, Toshihiko Imato, Takeshi Onodera, Kenshi Hayashi
    • Journal Title

      Transactions of the Materials Research Society of Japan

      Volume: 40

    • NAID

      130005089539

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] An Electrochemical Compact Disk-type Microfluidics Platform for Use as an Enzymatic Biosensor2015

    • Author(s)
      Poomrat Rattanarat, Prinjaporn Teengam, Weena Siangproh, Ryoichi Ishimatsu, Koji Nakano, Orawon Chailapakul, Toshihiko Imato
    • Journal Title

      Electroanalysis

      Volume: 27 Issue: 3 Pages: 703-712

    • DOI

      10.1002/elan.201400590

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fluorometric flow-immunoassay for alkylphenol polyethoxylates on a microchip containing a fluorescence detector comprised of an organic light emitting diode and an organic photodiode2015

    • Author(s)
      Rong Liu, Ryoichi Ishimatsu, Masayuki Yahiro, Chihaya Adachi, Koji Nakano, Toshihiko Imato
    • Journal Title

      Talanta

      Volume: 134 Pages: 37-47

    • DOI

      10.1016/j.talanta.2014.10.055

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Automated Chemiluminescence Immunoassay for a Nonionic Surfactant using a Recycled Spinning-pausing Controlled Washing Procedure on a Compact Disc-type Microfluidic Platform2015

    • Author(s)
      S. Guo, R. Ishimatsu, K. Nakano, T. Imato
    • Journal Title

      Talanta

      Volume: 133 Pages: 100-106

    • DOI

      10.1016/j.talanta.2014.06.075

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Photometric flow injection determination of phosphate on a PDMS microchip using an optical detection system assembled with an organic light emitting diode and an organic photodiode2015

    • Author(s)
      Rong Liu, Ryoichi Ishimatsu, Masayuki Yahiro, Chihaya Adachi, Koji Nakano, Toshihiko Imato
    • Journal Title

      Talanta

      Volume: 132 Pages: 96-105

    • DOI

      10.1016/j.talanta.2014.08.057

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 表面プラズモン共鳴センサを利用した 免疫グロブリンGのフローインジェクション分析2014

    • Author(s)
      石松亮一, 張瑞峰, 桐野侑子, 中野幸二, 今任稔彦
    • Journal Title

      Journal of Flow Injection Analysis

      Volume: 31 Pages: 111-114

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrogenerated Chemiluminescence of Donor-Acceptor Molecules Displaying Thermally Activated Delayed Fluorescence2014

    • Author(s)
      R. Ishimatsu, S. Matsunami, T. Kasahara, J. Mizuno, T. Edura, C. Adachi, K. Nakano, T. Imato
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 53 Issue: 27 Pages: 6996-6996

    • DOI

      10.1002/anie.201402615

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Potentiometric DNA Sensing Platform Using Redox-Active DNA Probe Pair for Sandwich-Type Dual Hybridization at Indicator Electrode Surface2014

    • Author(s)
      K. Nakano, T. Kimura, Y. Kitamura, T. Ihara, R. Ishimatsu, T. Imato
    • Journal Title

      Journal of Electroanalytical Chemistry

      Volume: 720-721 Pages: 71-75

    • DOI

      10.1016/j.jelechem.2014.03.029

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An organic thin film photodiode as a portable photodetector for the detection of alkylphenol polyethoxylates by a flow fluorescence-immunoassay on magnetic microbeads in a microchannel2013

    • Author(s)
      Ryoichi Ishimatsu, Azusa Naruse, Rong Liu, Koji Nakano, Masayuki Yahiro, Chihaya Adachi, Toshihiko Imato
    • Journal Title

      Talanta

      Volume: 117 Pages: 139-145

    • DOI

      10.1016/j.talanta.2013.08.044

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Solvent Effect on Thermally Activated Delayed Fluorescence by 1,2,3,5-Tetrakis(carbazol-9-yl)-4,6-dicyanobenzene2013

    • Author(s)
      Ryoichi Ishimatsu, Shigeyuki Matsunami, Katsuyuki Shizu, Chihaya Adachi, Koji Nakano, Toshihiko Imato
    • Journal Title

      The Journal of Physical Chemistry A

      Volume: 117 Issue: 27 Pages: 5607-5612

    • DOI

      10.1021/jp404120s

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Optical Sensing Systems Suitable for Flow Analysis on Microchips2013

    • Author(s)
      Rong Liu, Ryoichi Ishimatsu, Koji Nakano, Toshihiko Imato
    • Journal Title

      Journal of Flow Injection Analysis

      Volume: 30 Pages: 15-20

    • NAID

      40019892447

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Hisタグ融合TRPV1ゲスト結合モチーフペプチドからの自己組織化単分子膜形成とカプサイシンセンシング2014

    • Author(s)
      堀内潤,中野幸二,平田真吾,石松亮一,今任稔彦
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島市
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] フェロセン化デオキシヌクレオシドポリリン酸の合成とDNAを鋳型とする酵素反応の電気化学分析への応用2014

    • Author(s)
      田邊 潤壱,中野 幸二,平田 龍太郎,石松 亮一, 今任 稔彦
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島市
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Self-Assembly of Vanilloid-Binging Domain in Transient Receptor Potential Cation Channels based on His-Tag Chemistry at Gold Surfaces and Guest- Molecule Responses2014

    • Author(s)
      Koji Nakano, Shingo Hirata, Jun Horiuchi, Ryoichi Ishimatsu, Toshihiko Imato, Takeshi Onodera, Kenshi Hayashi
    • Organizer
      IUMRS-ICA2 014
    • Place of Presentation
      Fukuoka, Japana
    • Year and Date
      2014-08-24 – 2014-08-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Self- Assembly of Vanilloid-Binging Domain in Transient Receptor Potential Cation Channels based on His-Tag Chemistry at Gold Surfaces and Guest-Molecule Responses2014

    • Author(s)
      K. Nakano, S. Hirata, J. Horiuchi, R. Ishimatsu, T. Imato, T. Onodera, K. Hayashi
    • Organizer
      IUMRS-ICS 2014
    • Place of Presentation
      Fukuoka, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] 光架橋性分岐DNA二重らせんの自己組織化を利用するデンドリマー修飾電極の作製とその電気化学特性・バイオセンシング応用2013

    • Author(s)
      中野幸二、澤田貴文、森 美詞、山中 真、石松亮一、今任稔彦
    • Organizer
      日本分析化学会第67年会
    • Place of Presentation
      東大阪市
    • Related Report
      2013 Research-status Report
  • [Presentation] Synthesis of His-Tag Fused Vanilloid Receptor in Transient Receptor Potential Channels for Biosensing Application of Vanilloids2013

    • Author(s)
      Shingo Hirata, Koji Nakano, Jun Horiuchi, Ryoichi Ishimatsu, Toshihiko Imato, Kenshi Hayashi
    • Organizer
      The Twelfth Asian Conference on Analytical Sciences (ASIANALYSIS XII)
    • Place of Presentation
      Fukuoka, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] Hisタグ融合TRPV1ゲスト結合モチーフペプチドからの自己組織化単分子膜形成とバイオセンシング応用2013

    • Author(s)
      中野幸二、平田真吾、石松亮一、今任稔彦、林 健司
    • Organizer
      日本分析化学会第74回分析化学討論会
    • Place of Presentation
      函館市
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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