| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Glucose concentrations can be determined by using the coupled reaction system of glucose oxidase (GOx) and horseradish peroxidase (HRP). We integrated both GOx and HRP on an electrode surface through attachment at the termini of double-stranded oligodeoxynucleotides (ODNs). Reduction currents based on the coupled reactions were significantly increased on the electrode which tethered both enzymes by an interstrand crosslinked (ICL)-ODN. The current values were largely affected by the relative positions of enzymes on ICL-ODNs, and the detection sensitivity became the highest when GOx and HRP were distal and proximal to the electrode surface, respectively. The bienzyme/ICL-ODN complexes could also be constructed on a microelectrode surface and could also sensitively detect glucose concentrations. Here we report the construction of an electrode carrying bienzyme/ICL-ODN complex and the importance of enzyme binding positions on double stranded ODNs.
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