Development of non-fluorescent imaging technique for biological application

• Project/Area Number: 25620123
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: MOMOTAKE Atsuya 筑波大学, 数理物質系, 講師 (70375369)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: SHGイメージング / SHG顕微鏡 / 第二高調波発生 / バイオイメージング / アゾベンゼン / 膜染色 / 無発光性色素 / 細胞イメージング / 2光子顕微鏡 / 膜電位

Outline of Final Research Achievements

Live-cell imaging that combines multiple modalities had grown to be an essential technique in biological studies. In this context, we have focused on second harmonic generation (SHG) imaging technique, and have developed novel dyes that generate SHG signal but no fluorescence signal. SHG imaging technique has been shown to be useful in analyzing biological phenomena, the all dyes used so far for SHG imaging emit fluorescence and therefore multimodal imaging of these had been difficult. When the cell was stained directly with one of the dye, only SHG signals were detected without any fluorescence signal, indicating that these SHG-specific dyes do not interfere with fluorescence images of other fluorescent dyes. Therefore, with this new SHG specific dye, SHG and fluorescence imaging can be performed simultaneously without interference, enabling true multimodal imaging of biological phenomena for the first time.

Research Products

• [Journal Article] Evaluation of dialkylaminofluorene-based hemicyanine dyes for second harmonic generation imaging by the direct comparison approach. (2013)
  • Author(s): Nuriya, M, Momotake, A, Kimura, Y., Yasui, M., Kasai, H. & Arai, T.
  • Journal Title: Bull. Chemical Soc. Jpn. Volume: 86 Issue: 10 Pages: 1190-1192
  • DOI: 10.1246/bcsj.20130094
  • NAID: 10031201275
  • Peer Reviewed
• [Presentation] 光誘起電子移動型ケージド化合物の光分解反応効率に対する溶媒および犠牲還元剤の効果 (2015)
  • Author(s): 森田 里穂・百武 篤也・新井 達郎
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 日本大学船橋キャンパス
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 光誘起電子移動型ケージド化合物の光分解反応効率に対する電子ドナーの効果 (2014)
  • Author(s): 森田 里穂・百武 篤也・新井 達郎
  • Organizer: 光誘起電子移動型ケージド化合物の光分解反応効率に対する電子ドナーの効果
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-10-11 – 2014-10-13
• [Presentation] 光誘起電子移動型ケージド化合物の光分解反応と高機能化 (2014)
  • Author(s): 森田里穂・百武篤也・新井達郎
  • Organizer: 日本化学会第94春季年会(2014)
  • Place of Presentation: 名古屋大学東山キャンパス（名古屋）
• [Presentation] 光誘起生理活性物質放出を目指した機能性ケージド化合物の合成とその光化学 (2013)
  • Author(s): 本田 拓也・百武 篤也・新井 達郎
  • Organizer: 2013光化学討論会
  • Place of Presentation: 愛媛大学（松山）
• [Patent(Industrial Property Rights)] PCT Int. Appl. (2014)
  • Inventor(s): Atsuya Momotake他5名
  • Industrial Property Rights Holder: Atsuya Momotake他5名
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-05-23
  • Acquisition Date: 2014-11-27