| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In this research, we tried to develop novel type of nano-reactors to enhance enzyme properties, such as activity, life-time, stability, and so on. For this purpose, we exploited our original nano-compartments, PICsomes, which can be prepared in the aqueous media and encapsulate proteins inside based on a moderate loading method. Firstly, we succeeded in encapsulation of enzymes together with specific amount of macromolecular crowding reagents, dextrans. Furthermore, we found the stability enhancement of enzymes after encapsulation into nano-compartments. Also, enzymatic activity was preserved even after freezing of enzymatic nano-reactors, when macromolecular crowding reagents were co-encapsulated. Thus, we demonstrated potential utility of molecularly crowded nano-compartments for developing advanced and practically useful enzymatic nano-reactors.
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