New miRNA detecting system utilizing fluorescent DNA triplexes and rolling circle amplification
| Project/Area Number |
25620126
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Bio-related chemistry
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
SEIO KOHJI 東京工業大学, 生命理工学研究科, 准教授 (20313356)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 蛍光核酸 / microRNA / 三重鎖核酸 / 遺伝子検出 |
|---|
| Outline of Final Research Achievements |
We planed to develop new microRNA detection techniques using fluorescent oligonucleotides and rolling circle amplification. As the fluorescent nucleosides developed 5-(3-methylbenzofuran-2-yl)deoxyuridine (dUMBF), the dUMBF derivatives incorporating various substituents on its benzofuran ring, and 7-deazaguanine incorporating benzofuranyl group at the 7 position. The fluorescent properties of these nucleosides and oligonucleotides incorporating them were studied.
|
Report
(3 results)
Research Products
(7 results)
-
[Journal Article] Controlling the fluorescence of benzofuran-modified uracil residues in oligonucleotides by triple-helix formation2015
Author(s)
Takashi Kanamori, Hiroki Ohzeki, Yoshiaki Masaki, Akihiro Ohkubo, Mari Takahashi, Kengo Tsuda, Takuhiro Ito, Mikako Shirouzu, Kanako Kuwasako, Yutaka Muto, Mitsuo Sekine, Kohji Seio
-
Journal Title
Chembiochem
Volume: 16
Issue: 1
Pages: 167-176
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
-
-
