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Toward a novel method for constructing an artificial ribonucleopeptide enzyme

Research Project

Project/Area Number 25620131
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Bio-related chemistry
Research InstitutionKyoto University

Principal Investigator

MORII TAKASHI  京都大学, エネルギー理工学研究所, 教授 (90222348)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords人工酵素 / RNAアプタマー / RNA-ペプチド複合体 / ライブラリー / 活性スクリーニング / 基質結合場 / 分子認識能 / 協同的 / 反応モジュール / 基礎結合場
Outline of Final Research Achievements

A novel method for constructing an artificial enzyme by modification of the peptide subunit of ribonucleopeptide (RNP) with a catalytic group was investigated. We have developed a method to construct highly selective receptors for small molecules by using the RNP scaffold. The peptide subunit of RNP can be further functionalized with retaining the original molecular recognition of RNP receptor, for example, modification of the peptide with a fluorophore converts an RNP receptor to a fluorescent RNP sensor. In this study, an RNP library for the screening of catalytic activity was constructed by using an ATP-binding RNA library and a library of peptides modified at the N-terminal with a catalytic group through various kinds of peptide linkers. Screening of the RNP library for a hydrolytic activity afforded RNPs with enhanced activity.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2015 2014 2013 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Invited: 1 results)

  • [Journal Article] Peptidosteroid Tweezers Revisited: DNA Binding Through an Optimised Design2014

    • Author(s)
      Carrette, Lieselot L. G.; Morii, Takashi; Madder, Annemieke
    • Journal Title

      Eur. J. Org. Chem.

      Volume: 14 Issue: 14 Pages: 2883-2891

    • DOI

      10.1002/ejoc.201301854

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation regulates fibrillation of an aggregation core peptide in the second repeat of microtubule-binding domain of human tau2014

    • Author(s)
      M. Inoue, S. Kaida, S. Nakano, C. Annoni, E. Nakata, T. Konno, T. Morii
    • Journal Title

      Bioorg. Med. Chem。

      Volume: 22 Issue: 22 Pages: 6471-6480

    • DOI

      10.1016/j.bmc.2014.09.032

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells2013

    • Author(s)
      Shigeki Kiyonaka, Taketoshi Kajimoto, Reiko Sakaguchi, Daisuke Shinmi, Mariko Omatsu-Kanbe, Hiroshi Matsuura, Hiromi Imamura, Takenao Yoshizaki, Itaru Hamachi, Takashi Morii, Yasuo Mori
    • Journal Title

      Nature Methods

      Volume: 10 Issue: 12 Pages: 1232-1238

    • DOI

      10.1038/nmeth.2690

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Toxicity inspired cross-linking for probing DNA-peptide interactions2013

    • Author(s)
      Carrette, Lieselot L. G.; Morii, Takashi; Madder, Annemieke
    • Journal Title

      Bioconjugate Chemistry

      Volume: 24(12) Issue: 12 Pages: 2008-2014

    • DOI

      10.1021/bc400327q

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A protein adaptor to locate a functional protein dimer on molecular switchboard2013

    • Author(s)
      Tien Anh Ngo, Eiji Nakata, Masayuki Saimura, Tsutomu Kodaki, Takashi Morii
    • Journal Title

      Methods

      Volume: 0 Issue: 2 Pages: 1-9

    • DOI

      10.1016/j.ymeth.2013.10.014

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 触媒分子を導入したリボヌクレオペプチドライブラリーの活性スクリーニング2015

    • Author(s)
      田村友樹、有山健太、仲野 瞬、森井 孝
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      千葉
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 亜鉛(II)錯体を導入したリボヌクレオペプチドのエステル加水分解活性評価2015

    • Author(s)
      有山健太・田村友樹・仲野 瞬・森井 孝
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      千葉
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Ribonucleopeptides with catalytic functional groups2014

    • Author(s)
      T. Tamura, K. Ariyama, S. Nakano, T. Morii
    • Organizer
      ISNAC2014 第41回国際核酸化学シンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      2014-11-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 非天然活性中心を導入したリボヌクレオペプチドの触媒活性2014

    • Author(s)
      田村友樹,有山健太,仲野瞬,森井孝
    • Organizer
      第8回バイオ関連化学シンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Construction of Protein Assemblies on Molecular Switchboard

    • Author(s)
      森井 孝
    • Organizer
      A3RONA2013
    • Place of Presentation
      Konan University
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] DNAナノ構造体による機能性タンパク質組織体の構築

    • Author(s)
      森井 孝
    • Organizer
      高分子討論会
    • Place of Presentation
      金沢大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 共有結合化蛍光性RNPセンサーの同時利用

    • Author(s)
      仲野 瞬、劉 芳芳、田村友樹、中田栄司、森井 孝
    • Organizer
      第7回バイオ関連シンポジウム
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 反応活性中心を導入したリボヌクレオペプチドの触媒活性

    • Author(s)
      田村友樹、仲野 瞬、吉村祐輝、中田栄司、森井 孝
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学 東山キャンパス
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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