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Preparation and function of the membrane protein-reconstituted asymmetric liposomes

Research Project

Project/Area Number 25620138
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Bio-related chemistry
Research InstitutionKanagawa Academy of Science and Technology

Principal Investigator

KAMIYA Koki  公益財団法人神奈川科学技術アカデミー, 人工細胞膜システムグループ, 研究員 (70612315)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsリポソーム / 人工細胞モデル / 膜タンパク質 / フリップ-フロップ / 非対称膜 / ベシクル / リン脂質非対称 / マイクロデバイス / 脂質-タンパク質相互作用 / フリッパーゼ / ペプチド
Outline of Final Research Achievements

In this study, we investigated the flop dynamics of lipid asymmetric GV membranes in the presence of a membrane-interacted peptide. Our lipid asymmetric GVs were generated by a pulsed jet flow applied to a planar lipid bilayer. We observed that the DOPS-flop in asymmetric GV membranes was promoted in the presence of the peptides. Next, we successfully reconstituted flippases into the asymmetric GVs of phosphatidylserine. Finally, we observed the translocation of lipids from the extracellular leaflet to the cytoplasmic leaflet which was catalyzed by flippases. The asymmetric GVs will be useful for the study of elementary lipid-lipid or lipid-protein interactions.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (16 results)

All 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (8 results) (of which Invited: 1 results) Book (2 results) Remarks (4 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Single-Channel Current Measurement Of A Connexin Hemichannel Expressed Using An In Vitro Protein Synthesis System2014

    • Author(s)
      Koki Kamiya, Toshihisa Osaki, Ryuji Kawano, Minato Akiyama, Kazunari Akiyoshi, and Shoji Takeuchi
    • Journal Title

      Proceedings of MicroTAS

      Volume: 1 Pages: 918-919

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] 人工細胞作製に向けたリン脂質非対称膜リポソームの作製2014

    • Author(s)
      神谷厚輝、大崎寿久、竹内昌治
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Single-Channel Current Measurement Of A Connexin Hemichannel Expressed Using An In Vitro Protein Synthesis System2014

    • Author(s)
      Koki Kamiya, Toshihisa Osaki, Ryuji Kawano, Minato Akiyama, Kazunari Akiyoshi, and Shoji Takeuchi
    • Organizer
      MicroTAS 2014
    • Place of Presentation
      San Antonio
    • Year and Date
      2014-10-26 – 2014-10-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細胞サイズリン脂質非対称膜リポソームによる膜ダイナミク スの観察2014

    • Author(s)
      神谷厚輝、大崎寿久、柴崎宏介、竹内昌治
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] RECONSTITUTION AND FUNCTION OF MEMBRANE PROTEINS INTO ASYMMETRIC GIANT LIPOSOMES BY USING A PULSED JET FLOW2014

    • Author(s)
      K. Kamiya, R. Kawano, T. Osaki, and S. Takeuchi
    • Organizer
      MEMS2014
    • Place of Presentation
      San francisco America
    • Related Report
      2013 Research-status Report
  • [Presentation] リン脂質非対称膜リポソームへの膜タンパク質の再構成2014

    • Author(s)
      神谷厚輝、大崎寿久、川野竜司、竹内昌治
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 人工細胞は創れるか?-膜タンパク質再構成法とリン脂質非対称膜作製法2013

    • Author(s)
      神谷厚輝
    • Organizer
      日本機械学会北海道支部バイオメカニクス懇話会第13回講演会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 細胞サイズリン脂質非対象膜リポソームによるタンパク質-脂質相互作用観察2013

    • Author(s)
      神谷厚輝、川野竜司、大崎寿久、竹内昌治
    • Organizer
      第7回バイオ関連化学シンポジウム
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Research-status Report
  • [Presentation] PROTEIN EXPRESSION INSIDE OIL-FREE GIANT VESICLES BY USING PULSED JET FLOW METHOD2013

    • Author(s)
      K. Kamiya, R. Kawano, T. Osaki and S. Takeuchi
    • Organizer
      MicroTAS 2013
    • Place of Presentation
      Freiburg, Germany
    • Related Report
      2013 Research-status Report
  • [Book] Synthetic Biology2014

    • Author(s)
      Toshihisa Osaki, Koki Kamiya, and Shoji Takeuchi
    • Total Pages
      374
    • Publisher
      Royal Society of Chemistry
    • Related Report
      2014 Annual Research Report
  • [Book] 生体の科学2014

    • Author(s)
      神谷厚輝
    • Publisher
      医学書院
    • Related Report
      2014 Annual Research Report
  • [Remarks] 神奈川科技術アカデミー 人工細胞膜システムグループ

    • URL

      https://www.newkast.or.jp/innovation/labo/takeuchi_project.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 竹内昌治研究室

    • URL

      http://www.hybrid.iis.u-tokyo.ac.jp

    • Related Report
      2014 Annual Research Report
  • [Remarks] 神奈川科学技術アカデミー 竹内プロジェクト

    • URL

      http://www.newkast.or.jp/innovation/labo/takeuchi_project.html

    • Related Report
      2013 Research-status Report
  • [Remarks] 竹内昌治研究室

    • URL

      http://www.hybrid.iis.u-tokyo.ac.jp/

    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] リポソーム集団の製造方法2014

    • Inventor(s)
      神谷厚輝、竹内昌治、大崎寿久、川野竜司
    • Industrial Property Rights Holder
      神谷厚輝、竹内昌治、大崎寿久、川野竜司
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-118365
    • Filing Date
      2014-06-09
    • Related Report
      2014 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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