| Project/Area Number |
25620177
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Polymer/Textile materials
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO KAZUYA 鹿児島大学, 理工学域工学系, 准教授 (40347084)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | キチン／キトサン / 酵素触媒重合 / 立体異性体 / ホスホリラーゼ / 非天然型 / 酵素反応 |
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| Outline of Final Research Achievements |
This study performed the synthesis of chitin/chitosan stereoisomers by thermostable phosphorylase-catalyzed enzymatic polymerization. Phosphorylase catalyzes the enzymatic polymerization of α-D-glucose 1-phosphate (Glc-1-P) as a monomer to produce amylose with liberating inorganic phosphate. Because of loose specificity for the recognition of substrates, in this study, the thermostable phosphorylase-catalyzed enzymatic polymerization of α-D-glucosamine 1-phosphate (GlcN-1-P) as an analogue monomer was achieved under the conditions for removal of inorganic phosphate from the reaction media to give a non-natural aminopolysaccaride, that is, a chitosan stereoisomer. The product was further converted into a chitin stereoisomer by N-acetylation. The thermostable phosphorylase-catalyzed enzymatic copolymerization of Glc-1-P and GlcN-1-P as comonomers was also carried out to produce a non-natural glucosaminoglucan.
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