Development of a novel in situ hybridization method using gold nano particle for nanoSIMS analysis
| Project/Area Number |
25630221
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Tohoku University |
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Principal Investigator |
Kubota Kengo 東北大学, 工学研究科, 准教授 (80455807)
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| Co-Investigator(Renkei-kenkyūsha) |
MORONO YUKI 海洋研究開発機構, 主任研究員 (30421845)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 金粒子 / NanoSIMS / 微生物 / Ag沈着 / Undecagold |
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| Outline of Final Research Achievements |
We developed a novel in situ hybridization method using ultra-small gold nano-particle-labeled oligonucleotide probe for phylogenetic identification of environmental microorganisms during nanoSIMS analysis, and proposed as Gold-ISH. Lactate-assimilating sulfate reducing bacteria in an anaerobic wastewater treatment reactor were identified by using the proposed method.
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Report
(4 results)
Research Products
(4 results)
