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Development of a novel in situ hybridization method using gold nano particle for nanoSIMS analysis

Research Project

Project/Area Number 25630221
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Civil and environmental engineering
Research InstitutionTohoku University

Principal Investigator

Kubota Kengo  東北大学, 工学研究科, 准教授 (80455807)

Co-Investigator(Renkei-kenkyūsha) MORONO YUKI  海洋研究開発機構, 主任研究員 (30421845)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords金粒子 / NanoSIMS / 微生物 / Ag沈着 / Undecagold
Outline of Final Research Achievements

We developed a novel in situ hybridization method using ultra-small gold nano-particle-labeled oligonucleotide probe for phylogenetic identification of environmental microorganisms during nanoSIMS analysis, and proposed as Gold-ISH. Lactate-assimilating sulfate reducing bacteria in an anaerobic wastewater treatment reactor were identified by using the proposed method.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] 総論 水処理分野における顕微鏡観察2015

    • Author(s)
      久保田健吾
    • Journal Title

      水環境学会誌

      Volume: 38 Pages: 360-364

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Gold-ISH: A nano-size gold particle-based phylogenetic identification compatible with NanoSIMS2014

    • Author(s)
      Kengo Kubota, Yuki Morono, Motoo Ito, Takeshi Terada, Shogo Itezono, Hideki Harada, Fumio Inagaki
    • Journal Title

      Systematic and Applied Microbiology

      Volume: 37 Pages: 261-266

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] NanoSIMSを用いた微生物解析のための新規同定法2014

    • Author(s)
      久保田健吾, 諸野祐樹, 伊藤元雄
    • Organizer
      第17回日本水環境学会シンポジウム
    • Place of Presentation
      滋賀県立大学 (滋賀県彦根市)
    • Year and Date
      2014-09-09
    • Related Report
      2014 Research-status Report
  • [Presentation] NanoSIMS による微生物の系統学的同定と機能解明のためのISH 法の開発2014

    • Author(s)
      塚越大祐, 久保田健吾, 諸野祐樹, 伊藤元雄, 原田秀樹, 稲垣史生
    • Organizer
      日本水環境学会
    • Place of Presentation
      東北大学
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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