Development of novel bacterial growth inhibition technology for application of microbial process control

Research Project

Project/Area Number	25630222
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Civil and environmental engineering
Research Institution	Nagaoka University of Technology
Principal Investigator	HATAMOTO Masashi 長岡技術科学大学, 工学(系)研究科(研究院), 助教 (20524185)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	16S rRNA / アンチセンス / 人工核酸 / 増殖抑制 / 微生物 / 阻害 / メタン / 増殖阻害
Outline of Final Research Achievements	To evaluate an effect of anti-ribosome for microorganisms using LNA/DNA oligonucleotides, in vitro gene expression suppression experiments were conducted. From the experimental results, the designed LNA/DNA oligonucleotides had inhibitory effects depended on the dose concentrations, and the inhibitory effects were varied on its targeted site. So, as a next experiment pure bacterial cultures were used for inhibitory evaluations. From the results of growth inhibition experiments using E.coli strain AS19, little inhibitory effects were observed even thought higher concentration of LNA/DNA was used. Then, peptide added LNA/DNA oligonucleotides were designed and conducted the same experiment described above. The peptide added LNA/DNA oligonucleotides had a inhibitory effect in a certain extent but further research is needed to establish the microbial growth control technology for environmental engineering.