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Development of comprehensive quantification method for pathogenic bacteria that contributes to the clarification of the occurrence of pathogenic bacteria in the aquatic environment

Research Project

Project/Area Number 25630226
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Civil and environmental engineering
Research InstitutionKitasato University

Principal Investigator

INOUE Daisuke  北里大学, 医療衛生学部, 准教授 (70448091)

Co-Investigator(Kenkyū-buntansha) SEI Kazunari  北里大学, 医療衛生学部, 教授 (80324177)
IWASHITA Masato  北里大学, 医療衛生学部, 助教 (00137895)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords病原性細菌 / 水環境 / 網羅的定量解析 / DNAマイクロアレイ / DNAインターカレーター処理 / 網羅的解析 / DNAマイクロアレイ法
Outline of Final Research Achievements

This study aimed to develop a comprehensive quantification method for over 900 species of pathogenic bacteria and to clarify the occurrence of those pathogenic bacteria in the aquatic environment using the developed method. To these aims, this study tried to develop a selective detection method of viable bacteria in water using DNA intercalating treatment, to develop a quantitative DNA microarray method to simultaneously quantify over 900 pathogenic bacterial species, and to understand the occurrence of pathogenic bacteria in aquatic environments in Japan and other countries. Results of this study clarified the DNA intercalating treatment conditions useful for various bacterial species to some extent, and showed some important hints for the establishment of a quantitative DNA microarray method. In addition, this study could grasp the occurrence of over 900 pathogenic bacterial species in the Sagami River basin and in water samples collected from the Kathmandu valley, Nepal.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (9 results)

All 2015 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] High-throughput DNA microarray detection of pathogenic bacteria in shallow well groundwater in the Kathmandu Valley, Nepal2015

    • Author(s)
      Daisuke Inoue, Takuji Hinoura, Noriko Suzuki, Junqin Pang, Rabin Malla, Sadhana Shrestha, Saroj Kumar Chapagain, Hiroaki Matsuzawa, Takashi Nakamura, Yasuhiro Tanaka, Michihiko Ike, Kei Nishida, Kazunari Sei
    • Journal Title

      Current Microbiology

      Volume: 70 Issue: 1 Pages: 43-50

    • DOI

      10.1007/s00284-014-0681-x

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] ネパール・カトマンズ盆地の各種水試料中における病原性細菌汚染の実態調査2015

    • Author(s)
      吉永隼人, 井上大介, Bikash Malla, 田中靖浩, Jeevan B. Sherchand, 原本英司, 清和成
    • Organizer
      日本水処理生物学会第52回大会
    • Place of Presentation
      福岡県北九州市(北九州国際会議場)
    • Year and Date
      2015-11-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] Monitoring of bacterial pathogens in Sagami River basin for the grasp of the river water pollution by bacterial pathogens2015

    • Author(s)
      Kazunari Sei, Hayato Yoshinaga, Tomoharu Kamibayashi, Kaori Miyauchi, Takuji Hinoura, Daisuke Inoue
    • Organizer
      18th International Symposium on Health-Related Water Microbiology (WaterMicro2015)
    • Place of Presentation
      Lisbon, Portugal(Faculdade de Medicina Dentaria)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comprehensive analysis of pathogenic bacteria occurred in jar water, community well groundwater and river water in the Kathmandu Valley, Nepal2015

    • Author(s)
      Hayato Yoshinaga, Daisuke Inoue, Bikash Malla, Rajani Ghaju, Dinesh Bhandari, Sarmila Tandukar, Yasuhiro Tanaka, Jeevan B. Sherchand, Eiji Haramoto, Kazunari Sei
    • Organizer
      Water and Environment Technology Conference 2015
    • Place of Presentation
      東京都千代田区(日本大学駿河台キャンパス)
    • Year and Date
      2015-08-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DNAマイクロアレイを用いた相模川水系の病原性細菌汚染の調査2014

    • Author(s)
      吉永隼人, 上林智遥, 宮内華織, 日野浦拓之, 井上大介, 清和成
    • Organizer
      日本水処理生物学会第51回大会
    • Place of Presentation
      山梨県甲府市(山梨県JA会館)
    • Year and Date
      2014-11-12 – 2014-11-14
    • Related Report
      2014 Research-status Report
  • [Presentation] 様々な細菌種の生菌選択検出に適用可能なDNAインターカレーター処理法の開発2014

    • Author(s)
      井上大介, 上林智遥, 吉永隼人, 清和成
    • Organizer
      第48回日本水環境学会年会
    • Place of Presentation
      東北大学川内北キャンパス(宮城県仙台市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Comprehensive detection of pathogenic bacteria in shallow groundwater in Kathmandu Valley, Nepal, using DNA microarray2013

    • Author(s)
      Takuji Hinoura, Noriko Suzuki, Junqin Pang, Rabin Malla, Shrestha Malla, Saroj K. Chapagain, Hiroaki Matsuzawa, Yasuhiro Tanaka, Daisuke Inoue, Michihiko Ike, Kei Nishida, Kazunari Sei
    • Organizer
      6th Advanced Engineering Technology for Environment and Energy-Environment, Energy and Sustainable Development
    • Place of Presentation
      大阪大学吹田キャンパス(大阪府吹田市)
    • Related Report
      2013 Research-status Report
  • [Presentation] DNA microarray analysis of bacterial pathogens in shallow well groundwater in the Kathmandu Valley, Nepal2013

    • Author(s)
      Kazunari Sei, Takuji Hinoura, Kaori Miyauchi, Saroj K. Chapagain, Hiroaki Matsuzawa, Takashi Nakamura, Yasuhiro Tanaka, Daisuke Inoue, Kei Nishida
    • Organizer
      17th International Symposium on Health-Related Water Microbiology
    • Place of Presentation
      Majestic Palace Hotel (Florianopolis, Brazil)
    • Related Report
      2013 Research-status Report
  • [Presentation] DNAマイクロアレイを用いた相模原水系における病原性細菌分布の網羅的解析2013

    • Author(s)
      井上大介, 上林智遥, 吉永隼人, 宮内華織, 日野浦拓之, 清和成
    • Organizer
      日本水処理生物学会第50回大会
    • Place of Presentation
      神戸市水道局たちばな職員研修センター(兵庫県神戸市)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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