Formation of artificial nano-cells for in-situ TEM observation of material reaction in the water

• Project/Area Number: 25630269
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical properties of metals/Metal-base materials
• Research Institution: Tokyo University of Science
• Principal Investigator: Ishiguro Takkashi 東京理科大学, 基礎工学部, 教授 (10183162)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: バイオマテリアル / 水 / TEM / リポソーム / DPPC / リン脂質二重層膜 / コレステロール / ＴＥＭ / ＤＰＰＣ

Outline of Final Research Achievements

Liposome reinforced by adding cholesterol was synthesized using the dipalmitoylphosphatidylcholine (DPPC). Phase stability of the phospholipid bilayers was examined by FTIR spectroscopy. The reinforced liposomes filled with ultrapure water or physiological saline were observed by the conventional TEM equipped with a mass spectrometer. It was confirmed that the liposomes filled with water or saline solution were stable in the vacuum at room temperature. However、 during electron irradiation、 water molecules escaped gradually、 and a single crystal of rock salt precipitated in the liposome. Similar phenomenon was also observed in the case of cesium iodide solution.

Research Products

• [Journal Article] In-situ TEM observation of rock salt crystal precipitation in liposome (2016)
  • Author(s): Haruka Ai, Naoto Moriya, Takuji Ube, Takashi Harumoto, Yoshihiro Arai, Kazuyoshi Murata and Takashi Ishiguro
  • Journal Title: MRS Advances Volume: - Issue: 25 Pages: 1871-1876
  • DOI: 10.1557/adv.2016.277
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ultrafine nanoporous palladium-aluminum film fabricated by citric acid-assisted hot-water-treatment of aluminum-palladium alloy film (2015)
  • Author(s): Takashi Harumoto, Yohei Tamura and Takashi Ishiguro
  • Journal Title: AIP Advances Volume: 5 Issue: 1
  • DOI: 10.1063/1.4907049
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] In-situ Transmission Infrared Spectroscopy of Hydroxylation of Aluminum Thin Film and Subsequent Dehydration Processes (2014)
  • Author(s): Takuji Ube, Takashi Harumoto, Takashi Ishiguro
  • Journal Title: Jap. J. Appl. Phys. Volume: 53 Issue: 6 Pages: 066701-066701
  • DOI: 10.7567/jjap.53.066701
  • Peer Reviewed
• [Journal Article] Formation of Anti-Reflection Coating by Hydrothermal Treatment of Aluminum Films and Their Stabilization by Dehydration (2013)
  • Author(s): Aki Egashira, Takuji Ube, Yusuke Hosoki, Takashi Harumoto and Takashi Ishiguro
  • Journal Title: MATERIALS TRANSACTIONS Volume: 54 Issue: 6 Pages: 1025-1028
  • DOI: 10.2320/matertrans.M2013026
  • NAID: 10031176428
  • ISSN: 1345-9678, 1347-5320
  • Peer Reviewed
• [Presentation] In-situ TEM observation of rock salt crystal precipitation in liposome (2015)
  • Author(s): Haruka Ai, Naoto Moriya, Takuji Ube, Takashi Harumoto, Yoshihiro Arai, Kazuyoshi Murata and Takashi Ishiguro
  • Organizer: 2015 MRS Fall Meetings & Exhibit
  • Place of Presentation: Boston, Massachusetts, USA
  • Year and Date: 2015-11-29
  • Int'l Joint Research
• [Presentation] In-situ TEM observation of crystal precipitation in liposomes filled with sodium chloride aqueous solution (2014)
  • Author(s): Haruka Ai, Naoto Moriya, Takashi Harumoto, and Takashi Ishiguro
  • Organizer: 18th International Microscopy Congress
  • Place of Presentation: The Prague Congress Centre (Prague, Czech Republic)
  • Year and Date: 2014-09-07 – 2014-09-12
• [Presentation] Electron microscopic observation of antigen and antibody (2014)
  • Author(s): Atsushi Kawashimo, Takashi Harumoto, Akikazu Murakami, Takachika Azuma, and Takashi Ishiguro
  • Organizer: International Symposium on Technologies against Cancer 2014
  • Place of Presentation: Katsushika Campus, Tokyo University of Science, Tokyo, Japan
• [Presentation] 抗原抗体反応の電顕観察 (2014)
  • Author(s): 川下温, 春本高志, 村上明一, 東隆親, 石黒孝
  • Organizer: 2014 5th Biomedical Interface Workshop
  • Place of Presentation: 沖縄県石垣市商工会ホール