Electron transfer pathway regulating methane monooxygenase activity
| Project/Area Number |
25630363
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Catalyst/Resource chemical process
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
MIYAJI AKIMITSU 東京工業大学, 総合理工学研究科(研究院), 助教 (40452023)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | メタン水酸化 / 生体触媒 / 電子伝達 / 活性酸素 / メタン水酸化反応 |
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| Outline of Final Research Achievements |
In this study, we investigated the electron transfer pathway from the electron donor in bacteral membrane to the catalytic site of copper-containing methane monooxygenase. We extablished the method for removing a copper ion selectively from the mono-copper site of the copper-containing methane monooxygenase. The copper-deficient methane monooxygenase showed the activity for methane hydroxylation only when duroquinol, which can donate electrons directly to the catalytic site (di-copper site) of the copper-containing methane monooxygenase, was used as the electron donor. This result indicates that mono-copper site plays a role in the elctron mediation from the electron donor in bacterial membtarne to the catalytic site of copper-containing methane monooxygenase.
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Report
(4 results)
Research Products
(7 results)
