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High quality organelle recovery from microalgae with single cell disruption techniques

Research Project

Project/Area Number 25630372
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

TANAKA Tsuyoshi  東京農工大学, 工学(系)研究科(研究院), 教授 (20345333)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsオルガネラ回収 / 微細藻類 / 細胞破砕 / バイオディーゼル / 顕微ラマン分光法 / 微細孔アレイ / 単一細胞ライブイメージング / オルガネラ / 中性脂質 / 葉緑体 / 有用珪藻
Outline of Final Research Achievements

The cell disruption method to extract intact organelle from the cells, including hard plant cells, was developed in this study. The diatom species were used as model samples, and captured on the micro-cavity array, followed by addition of aspiration pressure. Another strategy for cell disruption is chemical method, such as acidic treatments. As comparing these two, an acidic treatment was demonstrated to be better way to achieve efficient and benign cell disruption. Furthermore, live imaging system for the micro-cavity array was developed, and further analysis and characterization were carried out using Raman microscope on the imaging system. These techniques can contribute to development of a quantitative and efficient cell disruption method.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (5 results)

  • [Journal Article] 1.In Vivo Live Cell Imaging for the Quantitative Monitoring of Lipids by Using Raman Microspectroscopy2014

    • Author(s)
      Masahito Hosokawa, Masahiro Ando, Shoichiro Mukai, Kyoko Osada, Tomoko Yoshino, Hiro-o Hamaguchi and Tsuyoshi Tanaka
    • Journal Title

      Analytical Chemistry

      Volume: 86 Issue: 16 Pages: 8224-8230

    • DOI

      10.1021/ac501591d

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Monitoring of Cellular Behaviors by Microcavity Array-Based Single-Cell Patterning2014

    • Author(s)
      Kyoko Osada, Masahito Hosokawa, Tomoko Yoshino, Tsuyoshi Tanaka
    • Journal Title

      Analyst

      Volume: 139 Issue: 2 Pages: 425-430

    • DOI

      10.1039/c3an01698f

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Lipid compositional analysis of marine diatom Fistulifera solaris strain JPCC DA0580 using Raman microspectroscopy2014

    • Author(s)
      Shoichiro Mukai
    • Organizer
      The Second Taiwan International Symposium On Raman Spectroscopy(2014)
    • Place of Presentation
      National Dong Hwa University(Hualien, Taiwan)
    • Year and Date
      2014-06-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 顕微ラマン分光法による海洋珪藻Fistulifera sp. JPCC DA0580株の脂質イメージング解析2013

    • Author(s)
      吉野知子
    • Organizer
      第15回マリンバイオテクノロジー学会大会
    • Place of Presentation
      沖縄県市町村自治会館(沖縄県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 単一細胞パターニングによる海洋珪藻Fistulifera sp. JPCC DA0580株のオイル蓄積過程のタイムラプス解析2013

    • Author(s)
      長田響子
    • Organizer
      第15回マリンバイオテクノロジー学会大会
    • Place of Presentation
      沖縄県市町村自治会館(沖縄県)
    • Related Report
      2013 Research-status Report
  • [Presentation] In vivo lipid analysis of marine diatom Fistulifera sp. strain JPCC DA0580 using Raman Microspectroscopy2013

    • Author(s)
      Shoichiro Mukai
    • Organizer
      The First Taiwan International Symposium on Raman Spectroscopy(2013)
    • Place of Presentation
      National Chiao Tung University (Taiwan)
    • Related Report
      2013 Research-status Report
  • [Presentation] 顕微ラマン分光法による海洋珪藻Fistulifera sp. JPCC DA0580株の脂質解析2013

    • Author(s)
      向井将一朗
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場(広島県)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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