• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Advanced hybridoma technology for selective generation of stereospecific monoclonal antibodies to GPCRs and its application

Research Project

Project/Area Number 25630376
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionMie University

Principal Investigator

Tomita Masahiro  三重大学, 工学研究科, 教授 (20183494)

Co-Investigator(Kenkyū-buntansha) 湊元 幹太  三重大学, 工学研究科, 准教授 (80362359)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsバイオテクノロジー / 生体分子 / 生体機能利用 / 免疫学 / 細胞・組織
Outline of Final Research Achievements

Based on next generation of hybridoma technology, we could successfully generate stereospecific monoclonal antibodies (ssmAbs) against stress-related human CRHR1, which belongs to one of GPCRs. After Cell-ELISA positive hybridoma cells were cloned by limiting dilution method, not only IgM-typed but also IgG-typed ssmAbs were obtained. Furthermore, this new technology was applicable to selective production of ssmAbs against other GPCR. Immunofluorescence analysis demonstrated that ssmAb specifically recognized intact hCRHR1 on the cell surface.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (112 results)

All 2016 2015 2014 2013 Other

All Journal Article (16 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 15 results,  Acknowledgement Compliant: 1 results,  Open Access: 3 results) Presentation (93 results) (of which Int'l Joint Research: 22 results,  Invited: 10 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Optimization of Pore Structure and Particle Morphology of Mesoporous Silica for Antibody Adsorption for Use in Affinity Chromatography.2016

    • Author(s)
      Hikosaka, R., Nagata, F., Tomita, M., Kato, K.
    • Journal Title

      Appl. Surf. Sci.

      Volume: 384 Pages: 27-35

    • DOI

      10.1016/j.apsusc.2016.05.016

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of the Pore Diameters and Amino-Organo Functional Structures on Mesoporous Silicas for DNA Adsorption.2016

    • Author(s)
      Hikosaka, R., Nagata, F., Tomita, M., Kato, K.
    • Journal Title

      Key Eng. Mater.

      Volume: 720 Pages: 31-36

    • DOI

      10.4028/www.scientific.net/kem.720.31

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The method used to culture host cells (Sf9 cells) can affect the qualities of baculovirus budding particles expressing recombinant proteins2016

    • Author(s)
      Tomomi Hattori, Kohei Nakanishi, Takaaki Mori, Masahiro Tomita, Kanta Tsumoto,
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 80 Issue: 3 Pages: 445-451

    • DOI

      10.1080/09168451.2015.1101331

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Adsorption and Desorption Characteristics of DNA onto the Surface of Amino Functional Mesoporous Silica with Various Particle Morphologies2016

    • Author(s)
      Ryouichi Hikosaka, Fukue Nagata, Masahiro Tomita, Katsuya kat
    • Journal Title

      Colloids Surf. B Biointerfaces

      Volume: 未定

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gold Nanoparticle-Mesoporous Silica Sheet Composites with Enhanced Antibody Adsorption Capacity2015

    • Author(s)
      Kazuma Nakanishi, Masahiro Tomita, Katsuya Kato
    • Journal Title

      New J. Chem.

      Volume: 39 Issue: 5 Pages: 4070-4077

    • DOI

      10.1039/c5nj00033e

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel mesoporous carbon sheets for electrochemical sensing of biological compounds2015

    • Author(s)
      Kazuma Nakanishi, Masahiro Tomita, Katsuya Kato
    • Journal Title

      MATERIALS LETTERS

      Volume: 160 Pages: 371-374

    • DOI

      10.1016/j.matlet.2015.08.008

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Preparation of mesoporous silica thin films by photocalcination method and their adsorrption abilities for various proteins2014

    • Author(s)
      Katsuya Kato
    • Journal Title

      Mater. Sci. Eng. C

      Volume: 40 Pages: 42-48

    • DOI

      10.1016/j.msec.2014.03.045

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Immunoprecipitation of bisphenol A by antibody-mesoporous silica composites2014

    • Author(s)
      Toru Orita
    • Journal Title

      J. Asian Ceram. Soc.

      Volume: 2 Issue: 3 Pages: 275-280

    • DOI

      10.1016/j.jascer.2014.05.010

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Strict targeting of receptors by stereospecific monoclonal antibodies2014

    • Author(s)
      Masahiro Tomita
    • Journal Title

      FEBS J.

      Volume: 281 (Suppl. 1) Pages: 503-504

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] pH Switching That Crosses over the Isoelectric Point (pI) Can Improve the Entrapment of Proteins within Giant Liposomes by Enhancing Protein-Membrane Interaction2014

    • Author(s)
      Takahiro Shimizu, Takaaki Mori, Masahiro Tomita and Kanta Tsumoto
    • Journal Title

      Langmuir

      Volume: 30 Issue: 2 Pages: 554-563

    • DOI

      10.1021/la403361j

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 人工細胞システム構成に役立てたい組換えプロテオリポソーム技術2014

    • Author(s)
      湊元幹太
    • Journal Title

      日本化学会・生体機能関連化学部会ニュースレター

      Volume: 28 Pages: 15-18

    • Related Report
      2013 Research-status Report
  • [Journal Article] Improvement of the catalytic activity of cytochrome c by immobilization on a novel mesoporous silica sheet2014

    • Author(s)
      Kazuma Nakanishi
    • Journal Title

      RSC Adv.

      Volume: 4 Issue: 9 Pages: 4732-4735

    • DOI

      10.1039/c3ra45861j

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A quantitative determination of magnetic nanoparticle separation using on-off fieldoperation of quadrupole magnetic field-flow fractionation (QMgFFF)2013

    • Author(s)
      Toru Orita
    • Journal Title

      Anal. Sci.

      Volume: 29 Pages: 761-764

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hydrothermal synthesis of hydroxyapatite nanoparticles and their protein adsorption behavior2013

    • Author(s)
      永田夫久江、山内善弘、冨田昌弘、加藤且也
    • Journal Title

      Journal of the Ceramic Society of Japan

      Volume: 121 Issue: 1417 Pages: 797-801

    • DOI

      10.2109/jcersj2.121.797

    • NAID

      130004480212

    • ISSN
      1348-6535, 1882-0743
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The binding of soluble recombinant human Fcγ receptor I for human immunoglobulin G is conferred by its first and second extracellular domains2013

    • Author(s)
      Yoshiharu Asaoka
    • Journal Title

      Mol. Immunol.

      Volume: 54 Issue: 3-4 Pages: 403-407

    • DOI

      10.1016/j.molimm.2013.01.007

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Specific binding of immunoglobulin G to protein A-mesoporous silica composites for affinity column chromatograpy2013

    • Author(s)
      Kazuma Nakanishi
    • Journal Title

      J. Mater. Chem. B

      Volume: 1 Issue: 45 Pages: 6321-6328

    • DOI

      10.1039/c3tb20998a

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Preparation and purification of GUVs with a reverse-phase method2016

    • Author(s)
      Yuki Hayashi, Masahiro Tomita, Kanta Tsumoto
    • Organizer
      10th Anniversary Internationla Symposium on Nanomedicine (ISNM2016)
    • Place of Presentation
      Auditorium of National Institute of Advanced Industrial Science and Technology (AIST) (Tsukuba)
    • Year and Date
      2016-11-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Visualization of membrane fusion between multicomponent protein-expressing BVs and GUVs prepared using a droplet-transfer method2016

    • Author(s)
      Misako Nishigami, Masahiro Tomita, Kanta Tsumoto
    • Organizer
      10th Anniversary Internationla Symposium on Nanomedicine (ISNM2016)
    • Place of Presentation
      Auditorium of National Institute of Advanced Industrial Science and Technology (AIST) (Tsukuba)
    • Year and Date
      2016-11-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Advanced hybridoma technology for stereospecific monoclonal antibodies as a next therapeutic medicine2016

    • Author(s)
      Yushi Isozaki, Hiroaki Miura, Kanta Tsumoto, Masahiro Tomita
    • Organizer
      10th Anniversary Internationla Symposium on Nanomedicine (ISNM2016)
    • Place of Presentation
      Auditorium of National Institute of Advanced Industrial Science and Technology (AIST) (Tsukuba)
    • Year and Date
      2016-11-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of the pore diameters and amino-organo functional structures2016

    • Author(s)
      Ryouichi Hikosaka, Fukue Nagata, Masahiro Tomita, and Katsuya Kato
    • Organizer
      28th Annual Meeting of the International Society for Ceramics in Medicine (Bioceramics 28)
    • Place of Presentation
      Charlotte, North Carolina, USA
    • Year and Date
      2016-10-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 次世代抗体医薬を目指した立体構造特異的モノクローナル抗体の作製およびその評価2016

    • Author(s)
      磯﨑勇志,三浦広己,湊元幹太,冨田昌弘
    • Organizer
      第 68 回日本生物工学会大会
    • Place of Presentation
      富山国際会議場(富山市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Stereospecific monoclonal antibodies as a new therapeutic medicine2016

    • Author(s)
      Yushi Isozaki, Hiroki Miura, Kanta Tsumoto, Masahiro Tomita
    • Organizer
      The 6th International Symposium for Sustainability by Engineering at MIU (IS2EMU2016) Research Area D & F Life Science &Advanced Materials
    • Place of Presentation
      三重大学工学部(津市)
    • Year and Date
      2016-09-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A novel method for direct preparation of liposomes from living cell materials2016

    • Author(s)
      Tsuyoshi Adachi, Masahiro Tomita, Kanta Tsumoto
    • Organizer
      The 6th International Symposium for Sustainability by Engineering at MIU (IS2EMU2016) Research Area D & F Life Science &Advanced Materials
    • Place of Presentation
      三重大学工学部(津市)
    • Year and Date
      2016-09-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Selective production of stereospecific monoclonal antibodies against receptors by SST method2016

    • Author(s)
      Shota Shimizu, Yushi Isozaki, Taka Sugiya, Kanta Tsumoto, Masahiro Tomita
    • Organizer
      The 6th International Symposium for Sustainability by Engineering at MIU (IS2EMU2016) Research Area D & F Life Science &Advanced Materials
    • Place of Presentation
      三重大学工学部(津市)
    • Year and Date
      2016-09-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Synthesis of amino functionalized mesoporous silica particles for RNA selective adsorption2016

    • Author(s)
      Ryouichi Hikosaka, Fukue Nagata, Masahiro Tomita, and Katsuya Kato
    • Organizer
      The 6th International Symposium for Sustainability by Engineering at MIU (IS2EMU2016) Research Area D & F Life Science &Advanced Materials
    • Place of Presentation
      三重大学工学部(津市)
    • Year and Date
      2016-09-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] インテグリンを再構成した巨大組換えプロテオリポソームの調製2016

    • Author(s)
      中西航平,西上美佐子,冨田昌弘,湊元幹太
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター/東北大学川内北キャンパス(仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] GPCR特異的立体構造認識モノクローナル抗体の効率的作製法の開発とその応用2016

    • Author(s)
      三浦広己,磯崎勇志,湊元幹太,冨田昌弘
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター/東北大学川内北キャンパス(仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] カルボキシル基修飾メソポーラスシリカ粒子の金属イオン吸着特性評価2016

    • Author(s)
      彦坂諒一,永田夫久江,冨田昌弘,加藤且也
    • Organizer
      公益社団法人日本セラミックス協会 第29回秋季シンポジウム
    • Place of Presentation
      広島大学(広島市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] カルボキシル基修飾メソポーラスシリカによるジスプロシウム分離カラムの創製2016

    • Author(s)
      彦坂諒一,永田夫久江,冨田昌弘,加藤且也
    • Organizer
      日本セラミックス協会東海支部 第52回東海若手セラミスト懇話会 2016年夏季セミナー
    • Place of Presentation
      十八楼(岐阜市)
    • Year and Date
      2016-06-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Recombinant Baculovirus Particles as Resources of Membrane Proteins for Artificial Cell Membrane Reconstitution2016

    • Author(s)
      Kanta Tsumoto, Kohei Nakanishi, Tomomi Hattori, Masahiro Tomita
    • Organizer
      The Fourth Japan-China Symposium on Nanomedicine
    • Place of Presentation
      Kitakyushu International Conference Center(Kitakyushu)
    • Year and Date
      2016-05-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 逆相法による巨大リン脂質ベシクルの形成と内外相の比重差を利用した単離2016

    • Author(s)
      林佑紀,冨田昌弘,湊元幹太
    • Organizer
      第80回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      三重大学講堂(津市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 組換えバキュロウイルスを用いたインテグリンプロテオリポソーム調製手法の検討2016

    • Author(s)
      中西航平,西上美佐子,冨田昌弘,湊元幹太
    • Organizer
      第80回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      三重大学講堂(津市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] ストレス関連受容体に対する立体構造認識抗体作製法の開発2016

    • Author(s)
      磯﨑勇志,三浦広己,湊元幹太,冨田昌弘
    • Organizer
      第80回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      三重大学講堂(津市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] GPCRに対する立体構造認識モノクローナル抗体作製技術の創製2016

    • Author(s)
      三浦広己,磯﨑勇志,湊元幹太,冨田昌弘
    • Organizer
      第80回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      三重大学講堂(津市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新しい分子標的治療薬をめざした立体構造特異的モノクローナル抗体の作製とその応用2016

    • Author(s)
      磯﨑勇志,三浦広己,湊元幹太,冨田昌弘
    • Organizer
      第6回地域イノベーション学会大会
    • Place of Presentation
      三重大学(津市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] バキュロウイルス膜融合法を用いた膜タンパク質再構成リポソームの作製と人工細胞モデルの構築2016

    • Author(s)
      中西航平,西上美佐子,冨田昌弘,湊元幹太
    • Organizer
      第6回地域イノベーション学会大会
    • Place of Presentation
      三重大学(津市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] アミノ基含有メソポーラスシリカの粒子サイズがRNA選択的吸着能に与える影響2016

    • Author(s)
      彦坂諒一,永田夫久江,冨田昌弘,加藤且也
    • Organizer
      平成28年度 日本セラミックス協会東海支部学術研究発表会
    • Place of Presentation
      名城大学(名古屋市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Hybridoma technology for selective production of multiple monoclonal antibodies2015

    • Author(s)
      H. Obata, K. Tsumoto, M. Tomita
    • Organizer
      9th International Symposium on Nanomedicine (ISNM2015)
    • Place of Presentation
      三重大学講堂(津市)
    • Year and Date
      2015-12-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Anti-GPCR monoclonal antibodies as novel antibody medicine2015

    • Author(s)
      Y. Isozaki, C. Miyamae, K. Tsumoto, M. Tomita
    • Organizer
      9th International Symposium on Nanomedicine (ISNM2015)
    • Place of Presentation
      三重大学講堂(津市)
    • Year and Date
      2015-12-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Selective generation of stereospecific monoclonal antibodies for the next generation of new therapeutic medicines2015

    • Author(s)
      C. Miyamae, Y. Isozaki, K. Tsumoto, M. Tomita
    • Organizer
      9th International Symposium on Nanomedicine (ISNM2015)
    • Place of Presentation
      三重大学講堂(津市)
    • Year and Date
      2015-12-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Visualization of the fusion between recombinant baculovirus and giant unilamellar vesicles prepared using a droplet-transfer method2015

    • Author(s)
      Misako Nishigami, Masahiro Tomita, Kanta Tsumoto
    • Organizer
      9th International Symposium on Nanomedicine (ISNM2015)
    • Place of Presentation
      三重大学講堂(津市)
    • Year and Date
      2015-12-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Qualities of recombinant baculovirus budding particles depend on the culture methods of host cells (Sf9 cells)2015

    • Author(s)
      T. Hattori, K. Nakanishi, M. Tomita, K. Tsumoto
    • Organizer
      9th International Symposium on Nanomedicine (ISNM2015)
    • Place of Presentation
      三重大学講堂(津市)
    • Year and Date
      2015-12-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Baculovirus-liposome membrane fusion by dehydration/rehydration: A novel method for proteoliposome preparation2015

    • Author(s)
      K. Nakanishi, M. Nishigami, M. Tomita, K. Tsumoto
    • Organizer
      9th International Symposium on Nanomedicine (ISNM2015)
    • Place of Presentation
      三重大学講堂(津市)
    • Year and Date
      2015-12-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Microscopic analysis on membrane fusion properties of baculovirus budded virus particles based on single GUV observation2015

    • Author(s)
      N. Nakagawa, M. Nishigami, M. Tomita, K. Tsumoto
    • Organizer
      9th International Symposium on Nanomedicine (ISNM2015)
    • Place of Presentation
      三重大学講堂(津市)
    • Year and Date
      2015-12-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Preparation of anti-IgG/mesoporous silica and the application as an affinity column for antibody purification2015

    • Author(s)
      R. Hikosaka, F. Nagata, M. Tomita, K. Kato
    • Organizer
      9th International Symposium on Nanomedicine (ISNM2015)
    • Place of Presentation
      三重大学講堂(津市)
    • Year and Date
      2015-12-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 脱水・再水和操作を用いた組換えバキュロウイルス膜融合法によるプロテオリポソーム調製2015

    • Author(s)
      中西航平、西上美佐子、冨田昌弘、湊元幹太
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会合同大会)
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 組換えバキュロウイルス出芽粒子の性状解析:宿主細胞培養条件の影響2015

    • Author(s)
      服部那美、中西航平、冨田昌弘、湊元幹太
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会合同大会)
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] SST法に基づく立体構造認識モノクローナル抗体の作製2015

    • Author(s)
      磯崎勇志、宮前智帆、湊元幹太、冨田昌弘
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会合同大会)
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 新規抗体医薬を目指した立体構造特異的ハイブリドーマテクノロジー2015

    • Author(s)
      宮前智帆、磯崎勇志、湊元幹太、冨田昌弘
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会合同大会)
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] プロテオリポソーム調製のための組換えバキュロウイルス-リポソーム新規膜融合法の検討2015

    • Author(s)
      中西航平、西上美佐子、冨田昌弘、湊元幹太
    • Organizer
      第46回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      三重大学工学部(津市)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
  • [Presentation] 単一GUV解析に基づくバキュロウイルス膜融合特性の基礎研究2015

    • Author(s)
      中川奈々、冨田昌弘、湊元幹太
    • Organizer
      第46回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      三重大学工学部(津市)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
  • [Presentation] インフルエンザウイルスの亜型迅速簡易キットをめざしたモノクローナル抗体作製2015

    • Author(s)
      島田靖士、尾中俊輝、湊元幹太、冨田昌弘
    • Organizer
      第46回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      三重大学工学部(津市)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
  • [Presentation] 次世代ハイブリドーマテクノロジーに基づく立体構造特異的モノクローナル抗体の作製2015

    • Author(s)
      宮前智帆、磯﨑勇志、湊元幹太、冨田昌弘
    • Organizer
      第46回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      三重大学工学部(津市)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
  • [Presentation] 抗体医薬に役立つ高効率細胞融合技術の開発2015

    • Author(s)
      伊藤駿、原吉彦、湊元幹太、冨田昌弘
    • Organizer
      第46回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      三重大学工学部(津市)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
  • [Presentation] マルチターゲティング法を利用した立体構造特異的抗体の同時作製2015

    • Author(s)
      小林洸貴、坂下健人、湊元幹太、冨田昌弘
    • Organizer
      第46回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      三重大学工学部(津市)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
  • [Presentation] 立体構造認識抗GPCR抗体の効率的作製2015

    • Author(s)
      磯﨑勇志、宮前智帆、湊元幹太、冨田昌弘
    • Organizer
      第46回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      三重大学工学部(津市)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
  • [Presentation] 脂質膜ベシクル:形成から細胞模倣まで2015

    • Author(s)
      湊元幹太
    • Organizer
      第46回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      三重大学工学部(津市)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 受容体特異的立体構造認識モノクローナル抗体の効率的作製2015

    • Author(s)
      磯﨑 勇志、宮前 智帆, 湊元 幹太, 冨田 昌弘
    • Organizer
      第67 回日本生物工学会大会
    • Place of Presentation
      城山観光ホテル(鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report
  • [Presentation] Preparation of recombinant proteoliposomes by dehydration/rehydration leading to fusion of baculovirus and liposome membranes2015

    • Author(s)
      Kohei Nakanishi, Misako Nishigami, Masahiro Tomita, Kanta Tsumoto
    • Organizer
      The 5th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2015)
    • Place of Presentation
      Graduate School of Engineering, Mie University(津市)
    • Year and Date
      2015-09-29
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Study on effects of preparation methods of recombinant protein-expressing baculovirus particles on their morphological properties2015

    • Author(s)
      Tomomi Hattori, Kohei Nakanishi, Masahiro Tomita, Kanta Tsumoto
    • Organizer
      The 5th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2015)
    • Place of Presentation
      Graduate School of Engineering, Mie University(津市)
    • Year and Date
      2015-09-29
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Advanced hybridoma technology for the next generation of therapeutic medicine2015

    • Author(s)
      Chiho Miyamae, Yushi Isozaki, Kanta Tsumoto, Masahiro Tomita
    • Organizer
      The 5th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2015)
    • Place of Presentation
      Graduate School of Engineering, Mie University(津市)
    • Year and Date
      2015-09-29
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Efficient production of stereospecific anti-GPCR monoclonal antibodies2015

    • Author(s)
      Yushi Isozaki, Chiho Miyamae, Kanta Tsumoto, Masahiro Tomita
    • Organizer
      The 5th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2015)
    • Place of Presentation
      Graduate School of Engineering, Mie University(津市)
    • Year and Date
      2015-09-29
    • Related Report
      2015 Research-status Report
  • [Presentation] Mesoporous silica functionalized with various aminosilanes as adsorbents for DNA2015

    • Author(s)
      Ryouichi Hikosaka, Masahiro Tomita, Katsuya Kato
    • Organizer
      The 5th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2015)
    • Place of Presentation
      Graduate School of Engineering, Mie University(津市)
    • Year and Date
      2015-09-29
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] A reverse phase method for the formation of giant vesicles (GVs) of phosphatidylcholine2015

    • Author(s)
      Kanta Tsumoto
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学角間キャンパス自然科学本館(金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
  • [Presentation] Adsorption of DNA on mesoporous silica functionalized with various aminosilanes2015

    • Author(s)
      Ryouichi Hikosaka, Masahiro Tomita, Katsuya Kato
    • Organizer
      The 5th International Conference on the Characterization and Control of Interfaces for High Quality Advanced Materials and the 51st Summer Symposium on Powder Technology (ICCCI2015)
    • Place of Presentation
      Hotel Nikko Kurashiki(岡山県、倉敷市)
    • Year and Date
      2015-07-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 有機鎖構造の異なるアミノ基修飾メソポーラスシリカ上へのDNAの吸着2015

    • Author(s)
      彦坂諒一、冨田昌弘,加藤且也
    • Organizer
      第50回東海若手セラミスト懇話会2015年夏季セミナー
    • Place of Presentation
      アヤハレークサイドホテル(滋賀県、大津市)
    • Year and Date
      2015-06-25
    • Related Report
      2015 Research-status Report
  • [Presentation] 非酸性条件でのバキュロウイルス膜融合法による組換えプロテオリポソーム調製の検討2015

    • Author(s)
      中西航平、西上美佐子、冨田昌弘、湊元幹太
    • Organizer
      第79回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      信州大学(松本キャンパス)旭総合研究棟(松本市)
    • Year and Date
      2015-05-23
    • Related Report
      2015 Research-status Report
  • [Presentation] 組換えバキュロウイルス出芽粒子の形態とタンパク発現:宿主細胞培養条件の影響2015

    • Author(s)
      服部那美、中西航平、冨田昌弘、湊元幹太
    • Organizer
      第79回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      信州大学(松本キャンパス)旭総合研究棟(松本市)
    • Year and Date
      2015-05-23
    • Related Report
      2015 Research-status Report
  • [Presentation] 新規抗体医薬を目指した立体構造特異的モノクローナル抗体の作製2015

    • Author(s)
      宮前智帆、磯﨑勇志、山﨑康裕、湊元幹太、冨田昌弘
    • Organizer
      第79回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      信州大学(松本キャンパス)旭総合研究棟(松本市)
    • Year and Date
      2015-05-23
    • Related Report
      2015 Research-status Report
  • [Presentation] 立体構造認識モノクローナル抗体作製のための新規抗体作製技術の創出2015

    • Author(s)
      磯﨑勇志、宮前智帆、山﨑康裕、加藤文法、宮田敬三、湊元幹太、冨田昌弘
    • Organizer
      第79回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      信州大学(松本キャンパス)旭総合研究棟(松本市)
    • Year and Date
      2015-05-23
    • Related Report
      2015 Research-status Report
  • [Presentation] 巨大リポソームによる細胞機能の再現2015

    • Author(s)
      湊元 幹太
    • Organizer
      第9回分子ナノテクノロジーセンター講演会
    • Place of Presentation
      兵庫県立大学姫路工学キャンパス (兵庫県姫路市)
    • Year and Date
      2015-03-19
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 人工細胞膜への機能性付与の1方法:組換えバキュロウイルス-リポソーム膜融合2015

    • Author(s)
      湊元 幹太
    • Organizer
      文部科学省科学技術人材育成費補助事業 テニュアトラック普及・定着事業・三重大学テニュアトラック普及・定着事業シンポジウム-研究者を目指す若手に新たな可能性の扉を開く-
    • Place of Presentation
      三重大学地域イノベーション研究開発拠点 (三重県津市)
    • Year and Date
      2015-03-06
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] A novel method for developing GUV artificial cell membranes displaying recombinant protein receptors with baculovirus expression systems2014

    • Author(s)
      Kanta Tsumoto
    • Organizer
      8th International Symposium on Nanomedicine (ISNM2014)
    • Place of Presentation
      Ehime University, Nanka Memeorial Hall (愛媛県松山市)
    • Year and Date
      2014-12-04 – 2014-12-06
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Stereospecific targeting of receptors2014

    • Author(s)
      Masahiro Tomita
    • Organizer
      8th International Symposium on Nanomedicine (ISNM2014)
    • Place of Presentation
      Ehime University, Nanka Memeorial Hall (愛媛県松山市)
    • Year and Date
      2014-12-04 – 2014-12-06
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 立体構造認識次世代ハイブリドーマテクノロジーの開発とその可視化解析2014

    • Author(s)
      冨田 昌弘
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜 (横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 立体構造認識抗体作製のための次世代ハイブリドーマテクノロジーの開発2014

    • Author(s)
      磯崎 勇志
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館 (京都市)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 立体構造特異的モノクローナル抗体作製に向けた感作B細胞の可視化解析2014

    • Author(s)
      宮前 智帆
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館 (京都市)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] インフルエンザ亜型特異的迅速簡易検査キットを目指した特異抗体の作製2014

    • Author(s)
      尾中 俊輝
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館 (京都市)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 宿主培養手順がバキュロウイルス出芽粒子上組換えタンパク質発現に与える影響2014

    • Author(s)
      服部 那美
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館 (京都市)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] バキュロウイルス膜融合法によるインテグリン再構成プロテオGUVの調製2014

    • Author(s)
      中西 航平
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館 (京都市)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Next generation of hybridoma technology for selective production of receptor-specific conformational monoclonal antibodies2014

    • Author(s)
      Yushi Isozaki
    • Organizer
      The 4th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2014)
    • Place of Presentation
      Mie University (三重県津市)
    • Year and Date
      2014-09-17 – 2014-09-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Integrin-reconstituted proteo-GUVs toward reconstitution of interactive cell models2014

    • Author(s)
      Kohei Nakanishi
    • Organizer
      The 4th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2014)
    • Place of Presentation
      Mie University (三重県津市)
    • Year and Date
      2014-09-17 – 2014-09-18
    • Related Report
      2014 Research-status Report
  • [Presentation] How do culture procedures of host cells (Sf9 cells) affect recombinant protein expressions on baculovirus budding particles?2014

    • Author(s)
      Tomomi Hattori
    • Organizer
      The 4th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2014)
    • Place of Presentation
      Mie University (三重県津市)
    • Year and Date
      2014-09-17 – 2014-09-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Immunofluorescence analysis of sensitized B lymphocytes for selective generation of stereospecific monoclonal antibodies2014

    • Author(s)
      Chiho Miyamae
    • Organizer
      The 4th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2014)
    • Place of Presentation
      Mie University (三重県津市)
    • Year and Date
      2014-09-17 – 2014-09-18
    • Related Report
      2014 Research-status Report
  • [Presentation] リポソーム工学をベースとする人工細胞システムの構築2014

    • Author(s)
      湊元 幹太
    • Organizer
      第66回日本生物工学会大会、シンポジウム(バイオベンチャーを創出する生体分子・バイオ界面工学のイノベーション)
    • Place of Presentation
      札幌コンベンションセンター (札幌市)
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Strict targeting of receptors by stereospecific monoclonal antibodies2014

    • Author(s)
      Masahiro Tomita
    • Organizer
      FEBS EMBO 2014 Conference
    • Place of Presentation
      Paris (France)
    • Year and Date
      2014-08-30 – 2014-09-04
    • Related Report
      2014 Research-status Report
  • [Presentation] Advanced hybridom technology for simultaneous production of novel monoclonal antibodies2014

    • Author(s)
      Haruka Obata
    • Organizer
      2014年度日本生物工学会中部支部例会
    • Place of Presentation
      名古屋大学工学研究科ES総合館1階ESホール (名古屋市)
    • Year and Date
      2014-08-10
    • Related Report
      2014 Research-status Report
  • [Presentation] Protein integration and encapsulation in giant liposomes2014

    • Author(s)
      Kanta Tsumoto
    • Organizer
      Development and future of artificial cells: Computation, fluctuation, and evolution
    • Place of Presentation
      Kyoto University, Maskawa Hall (京都市)
    • Year and Date
      2014-06-10
    • Related Report
      2014 Research-status Report
  • [Presentation] DNA免疫法に基づく感作B細胞の可視化解析2014

    • Author(s)
      宮前 智帆
    • Organizer
      第78回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      名古屋大学 野依記念学術交流会館 (名古屋市)
    • Year and Date
      2014-05-24
    • Related Report
      2014 Research-status Report
  • [Presentation] 次世代ハイブリドーマテクノロジーに基づく立体構造認識モノクローナル抗体作製法の開発2014

    • Author(s)
      磯崎 勇志
    • Organizer
      第78回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      名古屋大学 野依記念学術交流会館 (名古屋市)
    • Year and Date
      2014-05-24
    • Related Report
      2014 Research-status Report
  • [Presentation] バキュロウイルス出芽粒子調製における宿主細胞培養法の与える影響2014

    • Author(s)
      服部 那美
    • Organizer
      第78回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      名古屋大学 野依記念学術交流会館 (名古屋市)
    • Year and Date
      2014-05-24
    • Related Report
      2014 Research-status Report
  • [Presentation] インテグリンを再構成した巨大プロテオGUV調製の試み2014

    • Author(s)
      中西 航平
    • Organizer
      第78回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      名古屋大学 野依記念学術交流会館 (名古屋市)
    • Year and Date
      2014-05-24
    • Related Report
      2014 Research-status Report
  • [Presentation] Next generation of hybridoma technology for therapeutic monoclonal antibodies2014

    • Author(s)
      Masahiro Tomita
    • Organizer
      The 2nd ASIAN CLINICAL CONGRESS (ACC2)
    • Place of Presentation
      Kyoto Heian Hotel (京都市)
    • Year and Date
      2014-04-04 – 2014-04-05
    • Related Report
      2014 Research-status Report
  • [Presentation] 革新的高効率特異的抗体作製技術の迅速疾病診断への応用2014

    • Author(s)
      冨田 昌弘
    • Organizer
      新技術説明会
    • Place of Presentation
      JST 東京別館ホール,東京
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Next generation of therapeutic antibodies2014

    • Author(s)
      Masahiro Tomita
    • Organizer
      The eighteenth international conference on Human Antibodies & Hybridomas (HAH 2014)
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2013 Research-status Report
  • [Presentation] GPCR構成膜解析に向けたリガンド修飾ガラスの作製と結合評価2013

    • Author(s)
      安部裕也
    • Organizer
      第77回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      名古屋大,愛知
    • Related Report
      2013 Research-status Report
  • [Presentation] 次世代抗体医薬を目指したレセプター特異的立体構造認識モノクローナル抗体作製法の開発2013

    • Author(s)
      磯﨑勇志
    • Organizer
      第77回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      名古屋大,愛知
    • Related Report
      2013 Research-status Report
  • [Presentation] バキュロウイルス―巨大リポソーム膜融合によるGPCR経路要素組込み法の改良2013

    • Author(s)
      森貴昭
    • Organizer
      第77回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      名古屋大,愛知
    • Related Report
      2013 Research-status Report
  • [Presentation] 界面通過法GUVを用いたバキュロウイルス粒子の膜融合能の検討2013

    • Author(s)
      西上美佐子
    • Organizer
      第77回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      名古屋大,愛知
    • Related Report
      2013 Research-status Report
  • [Presentation] 組換えバキュロウイルス―巨大リポソーム膜融合によるGPCR経路要素の個別組込み法2013

    • Author(s)
      森貴昭
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜,神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] 界面通過法GUVを用いたバキュロウイルス粒子の膜融合能の検討と細胞模倣への利用2013

    • Author(s)
      西上美佐子
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜,神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] 次世代抗体医薬への応用を目指した新規はハイブリドーマテクノロジーの確立2013

    • Author(s)
      山﨑康裕
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜,神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] GPCR特異的立体構造認識モノクローナル抗体作製法の開発2013

    • Author(s)
      磯﨑勇志
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜,神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] CLSM analysis on the fusion between recombinant baculovirus and giant unilamellar vesicles prepared using a droplet-transfer method2013

    • Author(s)
      Misako Nishigami
    • Organizer
      The 3rd International Symposium for Sustainability by Engineering at MIU
    • Place of Presentation
      Mie University, Mie
    • Related Report
      2013 Research-status Report
  • [Presentation] Reconstruction of membrane protein components of signal pathways with membrane fusion between recombinant baculovirus and giant liposomes2013

    • Author(s)
      Takaaki Mori
    • Organizer
      The 3rd International Symposium for Sustainability by Engineering at MIU
    • Place of Presentation
      Mie University, Mie
    • Related Report
      2013 Research-status Report
  • [Presentation] Generation of species-specific monoclonal antibodies to Rickettsia japonica2013

    • Author(s)
      Shigehiro Akachi
    • Organizer
      The 3rd International Symposium for Sustainability by Engineering at MIU
    • Place of Presentation
      Mie University, Mie
    • Related Report
      2013 Research-status Report
  • [Presentation] Selective production of stereospecific monoclonal antibodies directed against GPCR2013

    • Author(s)
      Yushi Isozaki
    • Organizer
      The 3rd International Symposium for Sustainability by Engineering at MIU
    • Place of Presentation
      Mie University, Mie
    • Related Report
      2013 Research-status Report
  • [Presentation] Cell-sized liposomes: Basic structures for mimicking cell systems2013

    • Author(s)
      Kanta Tsumoto
    • Organizer
      7th International Symposium on Nanomedicine (ISNM2013)
    • Place of Presentation
      Kyushu Inst. Tech., Fukuoka
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] New hybridoma technology for selective production of stereospecific monoclonal antibodies2013

    • Author(s)
      Masahiro Tomita
    • Organizer
      7th International Symposium on Nanomedicine (ISNM2013)
    • Place of Presentation
      Kyushu Inst. Tech., Fukuoka
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] Production of monoclonal antibodies by the B-cell targeting technique; In: Culrure of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seven Edition (authoured/edited by R. Ian Freshney)2016

    • Author(s)
      Kanta Tsumoto,Yushi Isozaki, Masahiro Tomita
    • Total Pages
      728
    • Publisher
      John Wiley & Sons, Inc., Hoboken, NJ.
    • Related Report
      2015 Research-status Report
  • [Remarks] 三重大学大学院・工学研究科 分子生物工学研究室

    • URL

      http://www.bio.chem.mie-u.ac.jp/index.html

    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 微生物の迅速診断を可能とする特異抗体の高効率作製法2013

    • Inventor(s)
      冨田 昌弘
    • Industrial Property Rights Holder
      国立大学法人三重大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-209093
    • Filing Date
      2013-10-04
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi