Advanced hybridoma technology for selective generation of stereospecific monoclonal antibodies to GPCRs and its application

• Project/Area Number: 25630376
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Mie University
• Principal Investigator: Tomita Masahiro 三重大学, 工学研究科, 教授 (20183494)
• Co-Investigator(Kenkyū-buntansha): 湊元 幹太 三重大学, 工学研究科, 准教授 (80362359)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: バイオテクノロジー / 生体分子 / 生体機能利用 / 免疫学 / 細胞・組織

Outline of Final Research Achievements

Based on next generation of hybridoma technology, we could successfully generate stereospecific monoclonal antibodies (ssmAbs) against stress-related human CRHR1, which belongs to one of GPCRs. After Cell-ELISA positive hybridoma cells were cloned by limiting dilution method, not only IgM-typed but also IgG-typed ssmAbs were obtained. Furthermore, this new technology was applicable to selective production of ssmAbs against other GPCR. Immunofluorescence analysis demonstrated that ssmAb specifically recognized intact hCRHR1 on the cell surface.

Research Products

• [Journal Article] Optimization of Pore Structure and Particle Morphology of Mesoporous Silica for Antibody Adsorption for Use in Affinity Chromatography. (2016)
  • Author(s): Hikosaka, R., Nagata, F., Tomita, M., Kato, K.
  • Journal Title: Appl. Surf. Sci. Volume: 384 Pages: 27-35
  • DOI: 10.1016/j.apsusc.2016.05.016
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effect of the Pore Diameters and Amino-Organo Functional Structures on Mesoporous Silicas for DNA Adsorption. (2016)
  • Author(s): Hikosaka, R., Nagata, F., Tomita, M., Kato, K.
  • Journal Title: Key Eng. Mater. Volume: 720 Pages: 31-36
  • DOI: 10.4028/www.scientific.net/kem.720.31
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The method used to culture host cells (Sf9 cells) can affect the qualities of baculovirus budding particles expressing recombinant proteins (2016)
  • Author(s): Tomomi Hattori, Kohei Nakanishi, Takaaki Mori, Masahiro Tomita, Kanta Tsumoto,
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 80 Issue: 3 Pages: 445-451
  • DOI: 10.1080/09168451.2015.1101331
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Adsorption and Desorption Characteristics of DNA onto the Surface of Amino Functional Mesoporous Silica with Various Particle Morphologies (2016)
  • Author(s): Ryouichi Hikosaka, Fukue Nagata, Masahiro Tomita, Katsuya kat
  • Journal Title: Colloids Surf. B Biointerfaces Volume: 未定
  • Peer Reviewed
• [Journal Article] Gold Nanoparticle-Mesoporous Silica Sheet Composites with Enhanced Antibody Adsorption Capacity (2015)
  • Author(s): Kazuma Nakanishi, Masahiro Tomita, Katsuya Kato
  • Journal Title: New J. Chem. Volume: 39 Issue: 5 Pages: 4070-4077
  • DOI: 10.1039/c5nj00033e
  • Peer Reviewed
• [Journal Article] Novel mesoporous carbon sheets for electrochemical sensing of biological compounds (2015)
  • Author(s): Kazuma Nakanishi, Masahiro Tomita, Katsuya Kato
  • Journal Title: MATERIALS LETTERS Volume: 160 Pages: 371-374
  • DOI: 10.1016/j.matlet.2015.08.008
  • Peer Reviewed
• [Journal Article] Preparation of mesoporous silica thin films by photocalcination method and their adsorrption abilities for various proteins (2014)
  • Author(s): Katsuya Kato
  • Journal Title: Mater. Sci. Eng. C Volume: 40 Pages: 42-48
  • DOI: 10.1016/j.msec.2014.03.045
  • Peer Reviewed / Open Access
• [Journal Article] Immunoprecipitation of bisphenol A by antibody-mesoporous silica composites (2014)
  • Author(s): Toru Orita
  • Journal Title: J. Asian Ceram. Soc. Volume: 2 Issue: 3 Pages: 275-280
  • DOI: 10.1016/j.jascer.2014.05.010
  • Peer Reviewed / Open Access
• [Journal Article] Strict targeting of receptors by stereospecific monoclonal antibodies (2014)
  • Author(s): Masahiro Tomita
  • Journal Title: FEBS J. Volume: 281 (Suppl. 1) Pages: 503-504
  • Peer Reviewed / Open Access
• [Journal Article] pH Switching That Crosses over the Isoelectric Point (pI) Can Improve the Entrapment of Proteins within Giant Liposomes by Enhancing Protein-Membrane Interaction (2014)
  • Author(s): Takahiro Shimizu, Takaaki Mori, Masahiro Tomita and Kanta Tsumoto
  • Journal Title: Langmuir Volume: 30 Issue: 2 Pages: 554-563
  • DOI: 10.1021/la403361j
  • Peer Reviewed
• [Journal Article] 人工細胞システム構成に役立てたい組換えプロテオリポソーム技術 (2014)
  • Author(s): 湊元幹太
  • Journal Title: 日本化学会・生体機能関連化学部会ニュースレター Volume: 28 Pages: 15-18
• [Journal Article] Improvement of the catalytic activity of cytochrome c by immobilization on a novel mesoporous silica sheet (2014)
  • Author(s): Kazuma Nakanishi
  • Journal Title: RSC Adv. Volume: 4 Issue: 9 Pages: 4732-4735
  • DOI: 10.1039/c3ra45861j
  • Peer Reviewed
• [Journal Article] A quantitative determination of magnetic nanoparticle separation using on-off fieldoperation of quadrupole magnetic field-flow fractionation (QMgFFF) (2013)
  • Author(s): Toru Orita
  • Journal Title: Anal. Sci. Volume: 29 Pages: 761-764
  • Peer Reviewed
• [Journal Article] Hydrothermal synthesis of hydroxyapatite nanoparticles and their protein adsorption behavior (2013)
  • Author(s): 永田夫久江、山内善弘、冨田昌弘、加藤且也
  • Journal Title: Journal of the Ceramic Society of Japan Volume: 121 Issue: 1417 Pages: 797-801
  • DOI: 10.2109/jcersj2.121.797
  • NAID: 130004480212
  • ISSN: 1348-6535, 1882-0743
  • Peer Reviewed
• [Journal Article] The binding of soluble recombinant human Fcγ receptor I for human immunoglobulin G is conferred by its first and second extracellular domains (2013)
  • Author(s): Yoshiharu Asaoka
  • Journal Title: Mol. Immunol. Volume: 54 Issue: 3-4 Pages: 403-407
  • DOI: 10.1016/j.molimm.2013.01.007
  • Peer Reviewed
• [Journal Article] Specific binding of immunoglobulin G to protein A-mesoporous silica composites for affinity column chromatograpy (2013)
  • Author(s): Kazuma Nakanishi
  • Journal Title: J. Mater. Chem. B Volume: 1 Issue: 45 Pages: 6321-6328
  • DOI: 10.1039/c3tb20998a
  • Peer Reviewed
• [Presentation] Preparation and purification of GUVs with a reverse-phase method (2016)
  • Author(s): Yuki Hayashi, Masahiro Tomita, Kanta Tsumoto
  • Organizer: 10th Anniversary Internationla Symposium on Nanomedicine (ISNM2016)
  • Place of Presentation: Auditorium of National Institute of Advanced Industrial Science and Technology (AIST) (Tsukuba)
  • Year and Date: 2016-11-24
  • Int'l Joint Research
• [Presentation] Visualization of membrane fusion between multicomponent protein-expressing BVs and GUVs prepared using a droplet-transfer method (2016)
  • Author(s): Misako Nishigami, Masahiro Tomita, Kanta Tsumoto
  • Organizer: 10th Anniversary Internationla Symposium on Nanomedicine (ISNM2016)
  • Place of Presentation: Auditorium of National Institute of Advanced Industrial Science and Technology (AIST) (Tsukuba)
  • Year and Date: 2016-11-24
  • Int'l Joint Research
• [Presentation] Advanced hybridoma technology for stereospecific monoclonal antibodies as a next therapeutic medicine (2016)
  • Author(s): Yushi Isozaki, Hiroaki Miura, Kanta Tsumoto, Masahiro Tomita
  • Organizer: 10th Anniversary Internationla Symposium on Nanomedicine (ISNM2016)
  • Place of Presentation: Auditorium of National Institute of Advanced Industrial Science and Technology (AIST) (Tsukuba)
  • Year and Date: 2016-11-24
  • Int'l Joint Research
• [Presentation] Effect of the pore diameters and amino-organo functional structures (2016)
  • Author(s): Ryouichi Hikosaka, Fukue Nagata, Masahiro Tomita, and Katsuya Kato
  • Organizer: 28th Annual Meeting of the International Society for Ceramics in Medicine (Bioceramics 28)
  • Place of Presentation: Charlotte, North Carolina, USA
  • Year and Date: 2016-10-18
  • Int'l Joint Research
• [Presentation] 次世代抗体医薬を目指した立体構造特異的モノクローナル抗体の作製およびその評価 (2016)
  • Author(s): 磯﨑勇志，三浦広己，湊元幹太，冨田昌弘
  • Organizer: 第 68 回日本生物工学会大会
  • Place of Presentation: 富山国際会議場（富山市）
  • Year and Date: 2016-09-28
• [Presentation] Stereospecific monoclonal antibodies as a new therapeutic medicine (2016)
  • Author(s): Yushi Isozaki, Hiroki Miura, Kanta Tsumoto, Masahiro Tomita
  • Organizer: The 6th International Symposium for Sustainability by Engineering at MIU (IS2EMU2016) Research Area D & F Life Science &Advanced Materials
  • Place of Presentation: 三重大学工学部（津市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] A novel method for direct preparation of liposomes from living cell materials (2016)
  • Author(s): Tsuyoshi Adachi, Masahiro Tomita, Kanta Tsumoto
  • Organizer: The 6th International Symposium for Sustainability by Engineering at MIU (IS2EMU2016) Research Area D & F Life Science &Advanced Materials
  • Place of Presentation: 三重大学工学部（津市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] Selective production of stereospecific monoclonal antibodies against receptors by SST method (2016)
  • Author(s): Shota Shimizu, Yushi Isozaki, Taka Sugiya, Kanta Tsumoto, Masahiro Tomita
  • Organizer: The 6th International Symposium for Sustainability by Engineering at MIU (IS2EMU2016) Research Area D & F Life Science &Advanced Materials
  • Place of Presentation: 三重大学工学部（津市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] Synthesis of amino functionalized mesoporous silica particles for RNA selective adsorption (2016)
  • Author(s): Ryouichi Hikosaka, Fukue Nagata, Masahiro Tomita, and Katsuya Kato
  • Organizer: The 6th International Symposium for Sustainability by Engineering at MIU (IS2EMU2016) Research Area D & F Life Science &Advanced Materials
  • Place of Presentation: 三重大学工学部（津市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] インテグリンを再構成した巨大組換えプロテオリポソームの調製 (2016)
  • Author(s): 中西航平，西上美佐子，冨田昌弘，湊元幹太
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター／東北大学川内北キャンパス（仙台市）
  • Year and Date: 2016-09-25
• [Presentation] GPCR特異的立体構造認識モノクローナル抗体の効率的作製法の開発とその応用 (2016)
  • Author(s): 三浦広己，磯崎勇志，湊元幹太，冨田昌弘
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター／東北大学川内北キャンパス（仙台市）
  • Year and Date: 2016-09-25
• [Presentation] カルボキシル基修飾メソポーラスシリカ粒子の金属イオン吸着特性評価 (2016)
  • Author(s): 彦坂諒一，永田夫久江，冨田昌弘，加藤且也
  • Organizer: 公益社団法人日本セラミックス協会 第29回秋季シンポジウム
  • Place of Presentation: 広島大学（広島市）
  • Year and Date: 2016-09-07
• [Presentation] カルボキシル基修飾メソポーラスシリカによるジスプロシウム分離カラムの創製 (2016)
  • Author(s): 彦坂諒一，永田夫久江，冨田昌弘，加藤且也
  • Organizer: 日本セラミックス協会東海支部 第52回東海若手セラミスト懇話会 2016年夏季セミナー
  • Place of Presentation: 十八楼（岐阜市）
  • Year and Date: 2016-06-23
• [Presentation] Recombinant Baculovirus Particles as Resources of Membrane Proteins for Artificial Cell Membrane Reconstitution (2016)
  • Author(s): Kanta Tsumoto, Kohei Nakanishi, Tomomi Hattori, Masahiro Tomita
  • Organizer: The Fourth Japan-China Symposium on Nanomedicine
  • Place of Presentation: Kitakyushu International Conference Center（Kitakyushu）
  • Year and Date: 2016-05-12
  • Int'l Joint Research / Invited
• [Presentation] 逆相法による巨大リン脂質ベシクルの形成と内外相の比重差を利用した単離 (2016)
  • Author(s): 林佑紀，冨田昌弘，湊元幹太
  • Organizer: 第80回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 三重大学講堂（津市）
• [Presentation] 組換えバキュロウイルスを用いたインテグリンプロテオリポソーム調製手法の検討 (2016)
  • Author(s): 中西航平，西上美佐子，冨田昌弘，湊元幹太
  • Organizer: 第80回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 三重大学講堂（津市）
• [Presentation] ストレス関連受容体に対する立体構造認識抗体作製法の開発 (2016)
  • Author(s): 磯﨑勇志，三浦広己，湊元幹太，冨田昌弘
  • Organizer: 第80回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 三重大学講堂（津市）
• [Presentation] GPCRに対する立体構造認識モノクローナル抗体作製技術の創製 (2016)
  • Author(s): 三浦広己，磯﨑勇志，湊元幹太，冨田昌弘
  • Organizer: 第80回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 三重大学講堂（津市）
• [Presentation] 新しい分子標的治療薬をめざした立体構造特異的モノクローナル抗体の作製とその応用 (2016)
  • Author(s): 磯﨑勇志，三浦広己，湊元幹太，冨田昌弘
  • Organizer: 第6回地域イノベーション学会大会
  • Place of Presentation: 三重大学（津市）
• [Presentation] バキュロウイルス膜融合法を用いた膜タンパク質再構成リポソームの作製と人工細胞モデルの構築 (2016)
  • Author(s): 中西航平，西上美佐子，冨田昌弘，湊元幹太
  • Organizer: 第6回地域イノベーション学会大会
  • Place of Presentation: 三重大学（津市）
• [Presentation] アミノ基含有メソポーラスシリカの粒子サイズがRNA選択的吸着能に与える影響 (2016)
  • Author(s): 彦坂諒一，永田夫久江，冨田昌弘，加藤且也
  • Organizer: 平成28年度 日本セラミックス協会東海支部学術研究発表会
  • Place of Presentation: 名城大学（名古屋市）
• [Presentation] Hybridoma technology for selective production of multiple monoclonal antibodies (2015)
  • Author(s): H. Obata, K. Tsumoto, M. Tomita
  • Organizer: 9th International Symposium on Nanomedicine (ISNM2015)
  • Place of Presentation: 三重大学講堂（津市）
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] Anti-GPCR monoclonal antibodies as novel antibody medicine (2015)
  • Author(s): Y. Isozaki, C. Miyamae, K. Tsumoto, M. Tomita
  • Organizer: 9th International Symposium on Nanomedicine (ISNM2015)
  • Place of Presentation: 三重大学講堂（津市）
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] Selective generation of stereospecific monoclonal antibodies for the next generation of new therapeutic medicines (2015)
  • Author(s): C. Miyamae, Y. Isozaki, K. Tsumoto, M. Tomita
  • Organizer: 9th International Symposium on Nanomedicine (ISNM2015)
  • Place of Presentation: 三重大学講堂（津市）
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] Visualization of the fusion between recombinant baculovirus and giant unilamellar vesicles prepared using a droplet-transfer method (2015)
  • Author(s): Misako Nishigami, Masahiro Tomita, Kanta Tsumoto
  • Organizer: 9th International Symposium on Nanomedicine (ISNM2015)
  • Place of Presentation: 三重大学講堂（津市）
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] Qualities of recombinant baculovirus budding particles depend on the culture methods of host cells (Sf9 cells) (2015)
  • Author(s): T. Hattori, K. Nakanishi, M. Tomita, K. Tsumoto
  • Organizer: 9th International Symposium on Nanomedicine (ISNM2015)
  • Place of Presentation: 三重大学講堂（津市）
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] Baculovirus-liposome membrane fusion by dehydration/rehydration: A novel method for proteoliposome preparation (2015)
  • Author(s): K. Nakanishi, M. Nishigami, M. Tomita, K. Tsumoto
  • Organizer: 9th International Symposium on Nanomedicine (ISNM2015)
  • Place of Presentation: 三重大学講堂（津市）
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] Microscopic analysis on membrane fusion properties of baculovirus budded virus particles based on single GUV observation (2015)
  • Author(s): N. Nakagawa, M. Nishigami, M. Tomita, K. Tsumoto
  • Organizer: 9th International Symposium on Nanomedicine (ISNM2015)
  • Place of Presentation: 三重大学講堂（津市）
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] Preparation of anti-IgG/mesoporous silica and the application as an affinity column for antibody purification (2015)
  • Author(s): R. Hikosaka, F. Nagata, M. Tomita, K. Kato
  • Organizer: 9th International Symposium on Nanomedicine (ISNM2015)
  • Place of Presentation: 三重大学講堂（津市）
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] 脱水・再水和操作を用いた組換えバキュロウイルス膜融合法によるプロテオリポソーム調製 (2015)
  • Author(s): 中西航平、西上美佐子、冨田昌弘、湊元幹太
  • Organizer: BMB2015（第38回日本分子生物学会年会、第88回日本生化学会大会合同大会）
  • Place of Presentation: 神戸ポートアイランド（神戸市）
  • Year and Date: 2015-12-01
• [Presentation] 組換えバキュロウイルス出芽粒子の性状解析：宿主細胞培養条件の影響 (2015)
  • Author(s): 服部那美、中西航平、冨田昌弘、湊元幹太
  • Organizer: BMB2015（第38回日本分子生物学会年会、第88回日本生化学会大会合同大会）
  • Place of Presentation: 神戸ポートアイランド（神戸市）
  • Year and Date: 2015-12-01
• [Presentation] SST法に基づく立体構造認識モノクローナル抗体の作製 (2015)
  • Author(s): 磯崎勇志、宮前智帆、湊元幹太、冨田昌弘
  • Organizer: BMB2015（第38回日本分子生物学会年会、第88回日本生化学会大会合同大会）
  • Place of Presentation: 神戸ポートアイランド（神戸市）
  • Year and Date: 2015-12-01
• [Presentation] 新規抗体医薬を目指した立体構造特異的ハイブリドーマテクノロジー (2015)
  • Author(s): 宮前智帆、磯崎勇志、湊元幹太、冨田昌弘
  • Organizer: BMB2015（第38回日本分子生物学会年会、第88回日本生化学会大会合同大会）
  • Place of Presentation: 神戸ポートアイランド（神戸市）
  • Year and Date: 2015-12-01
• [Presentation] プロテオリポソーム調製のための組換えバキュロウイルス-リポソーム新規膜融合法の検討 (2015)
  • Author(s): 中西航平、西上美佐子、冨田昌弘、湊元幹太
  • Organizer: 第46回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 三重大学工学部（津市）
  • Year and Date: 2015-11-07
• [Presentation] 単一GUV解析に基づくバキュロウイルス膜融合特性の基礎研究 (2015)
  • Author(s): 中川奈々、冨田昌弘、湊元幹太
  • Organizer: 第46回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 三重大学工学部（津市）
  • Year and Date: 2015-11-07
• [Presentation] インフルエンザウイルスの亜型迅速簡易キットをめざしたモノクローナル抗体作製 (2015)
  • Author(s): 島田靖士、尾中俊輝、湊元幹太、冨田昌弘
  • Organizer: 第46回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 三重大学工学部（津市）
  • Year and Date: 2015-11-07
• [Presentation] 次世代ハイブリドーマテクノロジーに基づく立体構造特異的モノクローナル抗体の作製 (2015)
  • Author(s): 宮前智帆、磯﨑勇志、湊元幹太、冨田昌弘
  • Organizer: 第46回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 三重大学工学部（津市）
  • Year and Date: 2015-11-07
• [Presentation] 抗体医薬に役立つ高効率細胞融合技術の開発 (2015)
  • Author(s): 伊藤駿、原吉彦、湊元幹太、冨田昌弘
  • Organizer: 第46回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 三重大学工学部（津市）
  • Year and Date: 2015-11-07
• [Presentation] マルチターゲティング法を利用した立体構造特異的抗体の同時作製 (2015)
  • Author(s): 小林洸貴、坂下健人、湊元幹太、冨田昌弘
  • Organizer: 第46回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 三重大学工学部（津市）
  • Year and Date: 2015-11-07
• [Presentation] 立体構造認識抗GPCR抗体の効率的作製 (2015)
  • Author(s): 磯﨑勇志、宮前智帆、湊元幹太、冨田昌弘
  • Organizer: 第46回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 三重大学工学部（津市）
  • Year and Date: 2015-11-07
• [Presentation] 脂質膜ベシクル：形成から細胞模倣まで (2015)
  • Author(s): 湊元幹太
  • Organizer: 第46回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 三重大学工学部（津市）
  • Year and Date: 2015-11-07
  • Invited
• [Presentation] 受容体特異的立体構造認識モノクローナル抗体の効率的作製 (2015)
  • Author(s): 磯﨑 勇志、宮前 智帆, 湊元 幹太, 冨田 昌弘
  • Organizer: 第67 回日本生物工学会大会
  • Place of Presentation: 城山観光ホテル（鹿児島市）
  • Year and Date: 2015-10-26
• [Presentation] Preparation of recombinant proteoliposomes by dehydration/rehydration leading to fusion of baculovirus and liposome membranes (2015)
  • Author(s): Kohei Nakanishi, Misako Nishigami, Masahiro Tomita, Kanta Tsumoto
  • Organizer: The 5th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2015)
  • Place of Presentation: Graduate School of Engineering, Mie University（津市）
  • Year and Date: 2015-09-29
  • Int'l Joint Research
• [Presentation] Study on effects of preparation methods of recombinant protein-expressing baculovirus particles on their morphological properties (2015)
  • Author(s): Tomomi Hattori, Kohei Nakanishi, Masahiro Tomita, Kanta Tsumoto
  • Organizer: The 5th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2015)
  • Place of Presentation: Graduate School of Engineering, Mie University（津市）
  • Year and Date: 2015-09-29
  • Int'l Joint Research
• [Presentation] Advanced hybridoma technology for the next generation of therapeutic medicine (2015)
  • Author(s): Chiho Miyamae, Yushi Isozaki, Kanta Tsumoto, Masahiro Tomita
  • Organizer: The 5th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2015)
  • Place of Presentation: Graduate School of Engineering, Mie University（津市）
  • Year and Date: 2015-09-29
  • Int'l Joint Research
• [Presentation] Efficient production of stereospecific anti-GPCR monoclonal antibodies (2015)
  • Author(s): Yushi Isozaki, Chiho Miyamae, Kanta Tsumoto, Masahiro Tomita
  • Organizer: The 5th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2015)
  • Place of Presentation: Graduate School of Engineering, Mie University（津市）
  • Year and Date: 2015-09-29
• [Presentation] Mesoporous silica functionalized with various aminosilanes as adsorbents for DNA (2015)
  • Author(s): Ryouichi Hikosaka, Masahiro Tomita, Katsuya Kato
  • Organizer: The 5th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2015)
  • Place of Presentation: Graduate School of Engineering, Mie University（津市）
  • Year and Date: 2015-09-29
  • Int'l Joint Research
• [Presentation] A reverse phase method for the formation of giant vesicles (GVs) of phosphatidylcholine (2015)
  • Author(s): Kanta Tsumoto
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学角間キャンパス自然科学本館（金沢市）
  • Year and Date: 2015-09-13
• [Presentation] Adsorption of DNA on mesoporous silica functionalized with various aminosilanes (2015)
  • Author(s): Ryouichi Hikosaka, Masahiro Tomita, Katsuya Kato
  • Organizer: The 5th International Conference on the Characterization and Control of Interfaces for High Quality Advanced Materials and the 51st Summer Symposium on Powder Technology (ICCCI2015)
  • Place of Presentation: Hotel Nikko Kurashiki（岡山県、倉敷市）
  • Year and Date: 2015-07-07
  • Int'l Joint Research
• [Presentation] 有機鎖構造の異なるアミノ基修飾メソポーラスシリカ上へのDNAの吸着 (2015)
  • Author(s): 彦坂諒一、冨田昌弘，加藤且也
  • Organizer: 第50回東海若手セラミスト懇話会2015年夏季セミナー
  • Place of Presentation: アヤハレークサイドホテル（滋賀県、大津市）
  • Year and Date: 2015-06-25
• [Presentation] 非酸性条件でのバキュロウイルス膜融合法による組換えプロテオリポソーム調製の検討 (2015)
  • Author(s): 中西航平、西上美佐子、冨田昌弘、湊元幹太
  • Organizer: 第79回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 信州大学（松本キャンパス）旭総合研究棟（松本市）
  • Year and Date: 2015-05-23
• [Presentation] 組換えバキュロウイルス出芽粒子の形態とタンパク発現：宿主細胞培養条件の影響 (2015)
  • Author(s): 服部那美、中西航平、冨田昌弘、湊元幹太
  • Organizer: 第79回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 信州大学（松本キャンパス）旭総合研究棟（松本市）
  • Year and Date: 2015-05-23
• [Presentation] 新規抗体医薬を目指した立体構造特異的モノクローナル抗体の作製 (2015)
  • Author(s): 宮前智帆、磯﨑勇志、山﨑康裕、湊元幹太、冨田昌弘
  • Organizer: 第79回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 信州大学（松本キャンパス）旭総合研究棟（松本市）
  • Year and Date: 2015-05-23
• [Presentation] 立体構造認識モノクローナル抗体作製のための新規抗体作製技術の創出 (2015)
  • Author(s): 磯﨑勇志、宮前智帆、山﨑康裕、加藤文法、宮田敬三、湊元幹太、冨田昌弘
  • Organizer: 第79回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 信州大学（松本キャンパス）旭総合研究棟（松本市）
  • Year and Date: 2015-05-23
• [Presentation] 巨大リポソームによる細胞機能の再現 (2015)
  • Author(s): 湊元 幹太
  • Organizer: 第9回分子ナノテクノロジーセンター講演会
  • Place of Presentation: 兵庫県立大学姫路工学キャンパス (兵庫県姫路市)
  • Year and Date: 2015-03-19
  • Invited
• [Presentation] 人工細胞膜への機能性付与の1方法：組換えバキュロウイルス－リポソーム膜融合 (2015)
  • Author(s): 湊元 幹太
  • Organizer: 文部科学省科学技術人材育成費補助事業 テニュアトラック普及・定着事業・三重大学テニュアトラック普及・定着事業シンポジウム－研究者を目指す若手に新たな可能性の扉を開く－
  • Place of Presentation: 三重大学地域イノベーション研究開発拠点 (三重県津市)
  • Year and Date: 2015-03-06
  • Invited
• [Presentation] A novel method for developing GUV artificial cell membranes displaying recombinant protein receptors with baculovirus expression systems (2014)
  • Author(s): Kanta Tsumoto
  • Organizer: 8th International Symposium on Nanomedicine (ISNM2014)
  • Place of Presentation: Ehime University, Nanka Memeorial Hall (愛媛県松山市)
  • Year and Date: 2014-12-04 – 2014-12-06
  • Invited
• [Presentation] Stereospecific targeting of receptors (2014)
  • Author(s): Masahiro Tomita
  • Organizer: 8th International Symposium on Nanomedicine (ISNM2014)
  • Place of Presentation: Ehime University, Nanka Memeorial Hall (愛媛県松山市)
  • Year and Date: 2014-12-04 – 2014-12-06
  • Invited
• [Presentation] 立体構造認識次世代ハイブリドーマテクノロジーの開発とその可視化解析 (2014)
  • Author(s): 冨田 昌弘
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 (横浜市)
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 立体構造認識抗体作製のための次世代ハイブリドーマテクノロジーの開発 (2014)
  • Author(s): 磯崎 勇志
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館 (京都市)
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] 立体構造特異的モノクローナル抗体作製に向けた感作B細胞の可視化解析 (2014)
  • Author(s): 宮前 智帆
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館 (京都市)
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] インフルエンザ亜型特異的迅速簡易検査キットを目指した特異抗体の作製 (2014)
  • Author(s): 尾中 俊輝
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館 (京都市)
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] 宿主培養手順がバキュロウイルス出芽粒子上組換えタンパク質発現に与える影響 (2014)
  • Author(s): 服部 那美
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館 (京都市)
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] バキュロウイルス膜融合法によるインテグリン再構成プロテオGUVの調製 (2014)
  • Author(s): 中西 航平
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館 (京都市)
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Next generation of hybridoma technology for selective production of receptor-specific conformational monoclonal antibodies (2014)
  • Author(s): Yushi Isozaki
  • Organizer: The 4th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2014)
  • Place of Presentation: Mie University (三重県津市)
  • Year and Date: 2014-09-17 – 2014-09-18
• [Presentation] Integrin-reconstituted proteo-GUVs toward reconstitution of interactive cell models (2014)
  • Author(s): Kohei Nakanishi
  • Organizer: The 4th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2014)
  • Place of Presentation: Mie University (三重県津市)
  • Year and Date: 2014-09-17 – 2014-09-18
• [Presentation] How do culture procedures of host cells (Sf9 cells) affect recombinant protein expressions on baculovirus budding particles? (2014)
  • Author(s): Tomomi Hattori
  • Organizer: The 4th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2014)
  • Place of Presentation: Mie University (三重県津市)
  • Year and Date: 2014-09-17 – 2014-09-18
• [Presentation] Immunofluorescence analysis of sensitized B lymphocytes for selective generation of stereospecific monoclonal antibodies (2014)
  • Author(s): Chiho Miyamae
  • Organizer: The 4th International Symposium for Sustainability by Engineering at MIU (IS2EMU 2014)
  • Place of Presentation: Mie University (三重県津市)
  • Year and Date: 2014-09-17 – 2014-09-18
• [Presentation] リポソーム工学をベースとする人工細胞システムの構築 (2014)
  • Author(s): 湊元 幹太
  • Organizer: 第66回日本生物工学会大会、シンポジウム（バイオベンチャーを創出する生体分子・バイオ界面工学のイノベーション）
  • Place of Presentation: 札幌コンベンションセンター (札幌市)
  • Year and Date: 2014-09-09 – 2014-09-11
  • Invited
• [Presentation] Strict targeting of receptors by stereospecific monoclonal antibodies (2014)
  • Author(s): Masahiro Tomita
  • Organizer: FEBS EMBO 2014 Conference
  • Place of Presentation: Paris (France)
  • Year and Date: 2014-08-30 – 2014-09-04
• [Presentation] Advanced hybridom technology for simultaneous production of novel monoclonal antibodies (2014)
  • Author(s): Haruka Obata
  • Organizer: 2014年度日本生物工学会中部支部例会
  • Place of Presentation: 名古屋大学工学研究科ES総合館1階ESホール (名古屋市)
  • Year and Date: 2014-08-10
• [Presentation] Protein integration and encapsulation in giant liposomes (2014)
  • Author(s): Kanta Tsumoto
  • Organizer: Development and future of artificial cells: Computation, fluctuation, and evolution
  • Place of Presentation: Kyoto University, Maskawa Hall (京都市)
  • Year and Date: 2014-06-10
• [Presentation] DNA免疫法に基づく感作B細胞の可視化解析 (2014)
  • Author(s): 宮前 智帆
  • Organizer: 第７８回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 名古屋大学 野依記念学術交流会館 (名古屋市)
  • Year and Date: 2014-05-24
• [Presentation] 次世代ハイブリドーマテクノロジーに基づく立体構造認識モノクローナル抗体作製法の開発 (2014)
  • Author(s): 磯崎 勇志
  • Organizer: 第７８回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 名古屋大学 野依記念学術交流会館 (名古屋市)
  • Year and Date: 2014-05-24
• [Presentation] バキュロウイルス出芽粒子調製における宿主細胞培養法の与える影響 (2014)
  • Author(s): 服部 那美
  • Organizer: 第７８回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 名古屋大学 野依記念学術交流会館 (名古屋市)
  • Year and Date: 2014-05-24
• [Presentation] インテグリンを再構成した巨大プロテオGUV調製の試み (2014)
  • Author(s): 中西 航平
  • Organizer: 第７８回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 名古屋大学 野依記念学術交流会館 (名古屋市)
  • Year and Date: 2014-05-24
• [Presentation] Next generation of hybridoma technology for therapeutic monoclonal antibodies (2014)
  • Author(s): Masahiro Tomita
  • Organizer: The 2nd ASIAN CLINICAL CONGRESS (ACC2)
  • Place of Presentation: Kyoto Heian Hotel (京都市)
  • Year and Date: 2014-04-04 – 2014-04-05
• [Presentation] 革新的高効率特異的抗体作製技術の迅速疾病診断への応用 (2014)
  • Author(s): 冨田 昌弘
  • Organizer: 新技術説明会
  • Place of Presentation: JST 東京別館ホール，東京
  • Invited
• [Presentation] Next generation of therapeutic antibodies (2014)
  • Author(s): Masahiro Tomita
  • Organizer: The eighteenth international conference on Human Antibodies & Hybridomas (HAH 2014)
  • Place of Presentation: Vienna, Austria
• [Presentation] GPCR構成膜解析に向けたリガンド修飾ガラスの作製と結合評価 (2013)
  • Author(s): 安部裕也
  • Organizer: 第77回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 名古屋大，愛知
• [Presentation] 次世代抗体医薬を目指したレセプター特異的立体構造認識モノクローナル抗体作製法の開発 (2013)
  • Author(s): 磯﨑勇志
  • Organizer: 第77回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 名古屋大，愛知
• [Presentation] バキュロウイルス―巨大リポソーム膜融合によるGPCR経路要素組込み法の改良 (2013)
  • Author(s): 森貴昭
  • Organizer: 第77回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 名古屋大，愛知
• [Presentation] 界面通過法GUVを用いたバキュロウイルス粒子の膜融合能の検討 (2013)
  • Author(s): 西上美佐子
  • Organizer: 第77回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 名古屋大，愛知
• [Presentation] 組換えバキュロウイルス―巨大リポソーム膜融合によるGPCR経路要素の個別組込み法 (2013)
  • Author(s): 森貴昭
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜，神奈川
• [Presentation] 界面通過法GUVを用いたバキュロウイルス粒子の膜融合能の検討と細胞模倣への利用 (2013)
  • Author(s): 西上美佐子
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜，神奈川
• [Presentation] 次世代抗体医薬への応用を目指した新規はハイブリドーマテクノロジーの確立 (2013)
  • Author(s): 山﨑康裕
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜，神奈川
• [Presentation] GPCR特異的立体構造認識モノクローナル抗体作製法の開発 (2013)
  • Author(s): 磯﨑勇志
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜，神奈川
• [Presentation] CLSM analysis on the fusion between recombinant baculovirus and giant unilamellar vesicles prepared using a droplet-transfer method (2013)
  • Author(s): Misako Nishigami
  • Organizer: The 3rd International Symposium for Sustainability by Engineering at MIU
  • Place of Presentation: Mie University, Mie
• [Presentation] Reconstruction of membrane protein components of signal pathways with membrane fusion between recombinant baculovirus and giant liposomes (2013)
  • Author(s): Takaaki Mori
  • Organizer: The 3rd International Symposium for Sustainability by Engineering at MIU
  • Place of Presentation: Mie University, Mie
• [Presentation] Generation of species-specific monoclonal antibodies to Rickettsia japonica (2013)
  • Author(s): Shigehiro Akachi
  • Organizer: The 3rd International Symposium for Sustainability by Engineering at MIU
  • Place of Presentation: Mie University, Mie
• [Presentation] Selective production of stereospecific monoclonal antibodies directed against GPCR (2013)
  • Author(s): Yushi Isozaki
  • Organizer: The 3rd International Symposium for Sustainability by Engineering at MIU
  • Place of Presentation: Mie University, Mie
• [Presentation] Cell-sized liposomes: Basic structures for mimicking cell systems (2013)
  • Author(s): Kanta Tsumoto
  • Organizer: 7th International Symposium on Nanomedicine (ISNM2013)
  • Place of Presentation: Kyushu Inst. Tech., Fukuoka
  • Invited
• [Presentation] New hybridoma technology for selective production of stereospecific monoclonal antibodies (2013)
  • Author(s): Masahiro Tomita
  • Organizer: 7th International Symposium on Nanomedicine (ISNM2013)
  • Place of Presentation: Kyushu Inst. Tech., Fukuoka
  • Invited
• [Book] Production of monoclonal antibodies by the B-cell targeting technique; In: Culrure of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seven Edition (authoured/edited by R. Ian Freshney) (2016)
  • Author(s): Kanta Tsumoto,Yushi Isozaki, Masahiro Tomita
  • Total Pages: 728
  • Publisher: John Wiley & Sons, Inc., Hoboken, NJ.
• [Remarks] 三重大学大学院・工学研究科 分子生物工学研究室
  • URL: http://www.bio.chem.mie-u.ac.jp/index.html
• [Patent(Industrial Property Rights)] 微生物の迅速診断を可能とする特異抗体の高効率作製法 (2013)
  • Inventor(s): 冨田 昌弘
  • Industrial Property Rights Holder: 国立大学法人三重大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-209093
  • Filing Date: 2013-10-04