Development of fundamental technology to create E. coli carrying human-type glycosylation

• Project/Area Number: 25630378
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University
• Principal Investigator: FUJIYAMA Kazuhito 大阪大学, 生物工学国際交流センター, 教授 (70209112)
• Co-Investigator(Kenkyū-buntansha): OHASHI Takao 大阪大学, 生物工学国際交流センター, 助教 (10597876)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 大腸菌 / ヒト型化 / 糖鎖 / 組換え / ヒト型糖鎖 / 組み換えタンパク質

Outline of Final Research Achievements

We developed a fundamental E. coli strain for recombinant production of glycoprotein. Further improvement of this technology would enable E. coli to produce pharamaceutical glycoproteins, such as antibody.

Research Products

• [Journal Article] Production of initial-stage eukaryotic N-glycan and its protein glycosylation in Escherichia coli (2015)
  • Author(s): Srichaisupakit A, Ohashi T, Misaki R, Fujiyama K
  • Journal Title: J Biosci Bioeng. Volume: 119 Issue: 4 Pages: 399-405
  • DOI: 10.1016/j.jbiosc.2014.09.016
  • NAID: 110009942818
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of a protein glycosylation operon from Campylobacter jejuni JCM 2013 and its heterologous expression in Escherichia coli (2014)
  • Author(s): Srichaisupakit A, Ohashi T, Fujiyama K
  • Journal Title: J Biosci Bioeng. Volume: 未定 Issue: 3 Pages: 256-262
  • DOI: 10.1016/j.jbiosc.2014.02.011
  • NAID: 110009857354
  • Peer Reviewed / Open Access
• [Presentation] Identification of a protein glycosylation operon from Campylobacter jejuni JCM 2013 (2014)
  • Author(s): Srichaisupakit A, Ohashi T, Fujiyama K
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学生田キャンパス
• [Remarks] 藤山研究室 研究業績
  • URL: http://www.icb.osaka-u.ac.jp/fujiyama_lab/gyoseki.html